Mouse β-defensins 1 ELISA Kit

Mouse β-defensins 1 ELISA Kit

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中文名称：

小鼠β-防御素1酶联免疫试剂盒
货号：

CSB-E14188m
规格：

96T/48T
价格：

￥3600/￥2500
其他：

CUSABIO Wins 2024 Bioz Rapid Star™ Award in the ELISA category

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产品描述：

小鼠β-防御素1酶联免疫试剂盒（CSB-E14188m）为双抗夹心法ELISA试剂盒，定量检测血清、血浆、组织培养上清液、组织匀浆样本中的DEFB1含量。DEFB1（防御素β1）是一种抗菌肽，由中性粒细胞产生，与上皮表面对抗微生物定植的抵抗力有关。其基因表达与多种疾病相关，如龋齿、牙周炎和囊性纤维化。研究显示，DEFB1在男性生育过程中发挥促进精子活力和杀菌的双重作用，其缺陷可能导致弱精子症和精液白细胞增多症。试剂盒检测范围为15.6 pg/mL-1000 pg/mL，适用于科研场景中探究β-防御素1在感染性疾病模型、炎症反应机制或免疫调节研究中的表达变化，例如评估病原体刺激下组织局部防御素分泌动态、分析免疫相关基因敲除动物模型的黏膜屏障功能，或体外研究细胞因子对防御素表达的调控作用本品仅用于科研，不用于临床诊断，产品具体参数及操作步骤详见产品说明书。
别名：

Defb1Beta-defensin 1 ELISA kit; BD-1 ELISA kit; mBD-1 ELISA kit; Defensin ELISA kit; beta 1 ELISA kit
缩写：

DEFB1
Uniprot No.：

P56386
种属：

Mus musculus (Mouse)
样本类型：

serum, plasma, cell culture supernates, tissue homogenates
检测范围：

15.6 pg/mL-1000 pg/mL
灵敏度：

3.9 pg/mL
反应时间：

1-5h
样本体积：

50-100ul
检测波长：

450 nm
研究领域：

Microbiology
测定原理：

quantitative
测定方法：

Sandwich

精密度：

线性度：

To assess the linearity of the assay, samples were spiked with high concentrations of mouse β-defensins 1 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
	Sample	Serum(n=4)
1:5	Average %	93
	Range %	89-106
1:10	Average %	92
	Range %	83-100
1:20	Average %	95
	Range %	85-110
1:40	Average %	94
	Range %	89-103

回收率：

The recovery of mouse β-defensins 1 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.

Sample Type	Average % Recovery	Range
Serum (n=5)	97	91-103
EDTA plasma (n=4)	100	95-105

标准曲线：

These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

pg/ml	OD1	OD2	Average	Corrected
1000	2.184	2.052	2.118	1.936
500	1.363	1.377	1.370	1.188
250	0.890	0.861	0.876	0.694
125	0.517	0.482	0.500	0.318
62.5	0.375	0.364	0.370	0.188
31.2	0.286	0.267	0.277	0.095
15.6	0.231	0.225	0.228	0.046
0	0.184	0.179	0.182

数据处理：

ELISA操作视频及标曲绘制软件下载
货期：

3-5 working days

Dendrobium huoshanense polysaccharide regionally regulates intestinal mucosal barrier function and intestinal microbiota in mice Song-ZiXie, et al,Carbohydrate Polymers,2018

功能：

Has bactericidal activity. May act as a ligand for C-C chemokine receptor CCR6. Positively regulates the sperm motility and bactericidal activity in a CCR6-dependent manner. Binds to CCR6 and triggers Ca2+ mobilization in the sperm which is important for its motility.
基因功能参考文献：
1. Fenofibrate and gemfibrozil inhibit LPS-induced inflammatory activation of macrophages via PPARalpha/beta-defensin 1/TLR4 pathway. PMID: 25881202
2. these studies demonstrate a role for the murine beta-defensin 1 peptide in early control of Candida infection in a murine model of mucosal candidiasis PMID: 25595775
3. Akt1 is involved in the regulation of defensin expression and the innate immune response important for bacterial clearance PMID: 21559496
4. Histopathology showed a greater inflammatory influx in the lungs of mBD-1((-/-)) mice at Day 3 postinfection compared with WT C57BL/6 mice PMID: 21551252
5. The decreased production of antimicrobial peptides by burn-site epidermal keratinocytes influenced by Gr-1(+)CD11b(+) cells was shown to be restored by glycyrrhizin. PMID: 19843573
6. elimination of mBD-1 results in a defect in the ability of the host to clear H. influenzae from the lung, which is most consistent with this peptide functioning as an antibiotic at the airway surface. PMID: 12010999
7. Murine beta-defensins-1 and -4 were present in newborn skin. PMID: 12612195
8. The expression of BD1 was significantly elevated in the lungs of cigarette smoke-exposed mice compared with air-exposed mice. PMID: 18699806
9. Although both murine beta-defensin (mBD)-1 and mBD2 are constitutively expressed in normal BALB/c and C57BL/6 corneas, mBD-1 is not required for host resistance against Pseudomonas aeruginosa-induced corneal infection. PMID: 19155510
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亚细胞定位：

Secreted. Membrane.
蛋白家族：

Beta-defensin family
组织特异性：

Detected in kidney.
数据库链接：

KEGG: mmu:13214

STRING: 10090.ENSMUSP00000051754

UniGene: Mm.431316

Intra-assay Precision (Precision within an assay): CV%<8%

Three samples of known concentration were tested twenty times on one plate to assess.

Inter-assay Precision (Precision between assays): CV%<10%

Three samples of known concentration were tested in twenty assays to assess.