Mouse tumor necrosis factor-like ligand 1 (TL1) ELISA kit

Mouse tumor necrosis factor-like ligand 1 (TL1) ELISA kit

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中文名称：

小鼠肿瘤坏死因子配体相关分子-1(TL1)酶联免疫试剂盒
货号：

CSB-E16311m
规格：

96T/48T
价格：

￥3600/￥2500
其他：

CUSABIO Wins 2024 Bioz Rapid Star™ Award in the ELISA category

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CUSABIO小鼠肿瘤坏死因子配体相关分子-1（TL1）酶联免疫检测试剂盒（货号：CSB-E16311m），采用双抗体夹心法定量检测血清、血浆及组织匀浆样本中的TL1蛋白浓度。TL1作为肿瘤坏死因子超家族成员，在炎症反应和免疫调节中发挥重要作用，其异常表达与多种自身免疫性疾病的发生发展密切相关。试剂盒检测范围为62.5-4000 pg/mL，最低检测灵敏度达15.6 pg/mL，可在3.5小时内完成检测流程，所需样本量仅为50μL。该试剂盒在2-8℃条件下可稳定保存6个月，适用于科研实验中TL1蛋白表达水平的动态监测，例如炎症机制研究、免疫调控模型构建或药物干预效果评估等领域。通过标准曲线定量分析，研究者可精确测定不同生理或病理状态下样本中TL1的浓度变化，为探索该分子在免疫相关疾病中的作用提供可靠数据支持。
别名：

Tnfsf15 ELISA Kit; Tl1 ELISA Kit; Vegi ELISA Kit; Tumor necrosis factor ligand superfamily member 15 ELISA Kit; TNF ligand-related molecule 1 ELISA Kit; Vascular endothelial cell growth inhibitor ELISA Kit
缩写：

TNFSF15
Uniprot No.：

Q5UBV8
种属：

Mus musculus (Mouse)
样本类型：

serum, plasma, tissue homogenates
检测范围：

62.5 pg/mL-4000 pg/mL
灵敏度：

15.6 pg/mL
反应时间：

1-5h
样本体积：

50-100ul
检测波长：

450 nm
研究领域：

Others
测定原理：

quantitative
测定方法：

Sandwich

精密度：

线性度：

To assess the linearity of the assay, samples were spiked with high concentrations of mouse TL1 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
	Sample	Serum(n=4)
1:1	Average %	96
	Range %	90-102
1:2	Average %	89
	Range %	84-95
1:4	Average %	95
	Range %	90-100
1:8	Average %	104
	Range %	100-108

回收率：

The recovery of mouse TL1 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.

Sample Type	Average % Recovery	Range
Serum (n=5)	87	82-93
EDTA plasma (n=4)	94	87-98

标准曲线：

These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

pg/ml	OD1	OD2	Average	Corrected
4000	2.871	2.641	2.756	2.601
2000	2.086	2.067	2.077	1.922
1000	1.348	1.325	1.337	1.182
500	0.811	0.826	0.819	0.664
250	0.505	0.544	0.525	0.370
125	0.293	0.299	0.296	0.141
62.5	0.219	0.235	0.227	0.072
0	0.154	0.156	0.155

数据处理：

ELISA操作视频及标曲绘制软件下载
货期：

3-5 working days

T cell expression of IL-18R and DR3 is essential for non-cognate stimulation of Th1 cells and optimal clearance of intracellular bacteria Pham OH.et al,PLoS Pathog,2017
Tumor necrosis factor superfamily member 15 (Tnfsf15) facilitates lymphangiogenesis via upregulation of Vegfr3 gene expression in lymphatic endothelial cells Qin TT. et al,J Pathol,2015
S100A9: A Potential Biomarker for the Progression of Non-Alcoholic Fatty Liver Disease and the Diagnosis of Non-Alcoholic Steatohepatitis Liu X. et al,PLoS One,2015

功能：

Receptor for TNFRSF25 and TNFRSF6B. Mediates activation of NF-kappa-B. Inhibits vascular endothelial growth and angiogenesis (in vitro). Promotes activation of caspases and apoptosis. Promotes splenocyte alloactivation.
基因功能参考文献：
1. TL1A blocking ameliorates intestinal fibrosis in the T cell transfer model of chronic colitis PMID: 29254800
2. ATF3 protects against LPS-induced acute lung injury by inhibiting TL1A expression. PMID: 28177121
3. this study shows that TL1A-/- mice are more susceptible to dextran sodium sulfate colitis PMID: 27233964
4. These data demonstrated a direct role for TL1A-DR3 signaling in tissue fibrosis and that modulation of TL1A-DR3 signaling could inhibit gut fibrosis. PMID: 24850426
5. TL1A deficiency impacts on the gut microbial composition and the mucosal immune system, especially the intraepithelial TCRgammadelta(+) T-cell subset, and that TL1A is involved in the establishment of adipose tissue. PMID: 25404161
6. Our data demonstrate a key role for TL1A in promoting ILC2s at mucosal barriers. PMID: 24220298
7. delivers survival signals to plasma cells and promotes pathogenic Ab production in collagen arthritis PMID: 24140642
8. TNFSF15 inhibits vasculogenesis by regulating relative levels of membrane-bound and soluble isoforms of VEGF receptor 1. PMID: 23918400
9. These results establish a novel link between tumor necrosis factor (TNF)-family cytokine TL1A (TNFSF15) and interleukin 13 (IL-13) responses that results in small intestinal inflammation. PMID: 20980995
10. role of TL1A in mucosal T cells and antigen presenting cells function and showed that up-regulation of TL1A expression can promote mucosal inflammation and gut fibrosis. PMID: 21264313
11. findings suggest that the transcription factor NF-kappaB plays an important role in the regulation of VEGI expression PMID: 15702971
12. neonatal CD4+ CD3- cells up-regulate both CD30L and OX40L after adoptive transfer into an adult recipient PMID: 16920944
13. VEGI may have a key role in both the termination of angiogenesis and facilitation of dendritic cell maturation, which is the initial step in immune responses toward inflammatory stimuli. PMID: 17785811
14. TL1A may contribute to renal inflammation and injury through DR3-mediated activation of NF-kappaB and caspase-3 PMID: 18287561
15. Results reveal that TL1A is an important modulator in the development of chronic mucosal inflammation by enhancing T(H)1 and T(H)17 effector functions. PMID: 18598698
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亚细胞定位：

Membrane; Single-pass type II membrane protein.
蛋白家族：

Tumor necrosis factor family
数据库链接：

KEGG: mmu:326623

STRING: 10090.ENSMUSP00000050144

UniGene: Mm.208152

Intra-assay Precision (Precision within an assay): CV%<8%

Three samples of known concentration were tested twenty times on one plate to assess.

Inter-assay Precision (Precision between assays): CV%<10%

Three samples of known concentration were tested in twenty assays to assess.