Mouse tissue inhibitors of metalloproteinase 2,TIMP-2 ELISA kit

产品详情

Intra-assay Precision (Precision within an assay): CV%<8%

Three samples of known concentration were tested twenty times on one plate to assess.

Inter-assay Precision (Precision between assays): CV%<10%

Three samples of known concentration were tested in twenty assays to assess.

产品描述：

小鼠基质金属蛋白酶抑制因子2(TIMP-2)酶联免疫试剂盒（CSB-E07387m）为双抗夹心法ELISA试剂盒，定量检测血清、血浆、组织培养上清液、组织匀浆样本中的TIMP2含量。TIMP2 是组织金属蛋白酶抑制因子 2，在体内能抑制金属蛋白酶活性，维持细胞外基质稳态。其研究机制主要聚焦于它在肿瘤发生、发展中作用，如抑制肿瘤细胞侵袭、转移，还参与血管生成调控，有望成为癌症等疾病治疗新靶点。试剂盒检测范围为125 pg/mL-8000 pg/mL，适用于体外细胞实验、动物模型研究及组织样本分析，为探究TIMP-2在炎症反应、肿瘤微环境调控或器官纤维化等领域的分子机制提供可靠工具，适用于分子生物学、病理机制研究等科研场景。本品仅用于科研，不用于临床诊断，产品具体参数及操作步骤详见产品说明书。
别名：

Timp2 ELISA Kit; Timp-2 ELISA Kit; Metalloproteinase inhibitor 2 ELISA Kit; Tissue inhibitor of metalloproteinases 2 ELISA Kit; TIMP-2 ELISA Kit
缩写：

TIMP2
Uniprot No.：

P25785
种属：

Mus musculus (Mouse)
样本类型：

serum, plasma, cell culture supernates, tissue homogenates
检测范围：

125 pg/mL-8000 pg/mL
灵敏度：

31.25 pg/mL
反应时间：

1-5h
样本体积：

50-100ul
检测波长：

450 nm
研究领域：

Cancer
测定原理：

quantitative
测定方法：

Sandwich

精密度：

线性度：

To assess the linearity of the assay, samples were spiked with high concentrations of mouse TIMP-2 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
	Sample	Serum(n=4)
1:20	Average %	106
	Range %	101-113
1:40	Average %	84
	Range %	80-88
1:80	Average %	95
	Range %	90-100
1:160	Average %	98
	Range %	94-105

回收率：

The recovery of mouse TIMP-2 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.

Sample Type	Average % Recovery	Range
Serum (n=5)	91	85-96
EDTA plasma (n=4)	88	84-93

标准曲线：

These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

功能：

Complexes with metalloproteinases (such as collagenases) and irreversibly inactivates them by binding to their catalytic zinc cofactor.
亚细胞定位：

Secreted.
蛋白家族：

Protease inhibitor I35 (TIMP) family
组织特异性：

Detected in testis, retina, hippocampus and cerebral cortex.
数据库链接：

STRING: 10090.ENSMUSP00000017610

UniGene: Mm.206505