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Mouse terminal complement complex C5b-9,TCC C5b-9 ELISA Kit

  • 中文名称:
    小鼠末端补体复合物C5b-9(TCC C5b-9)酶联免疫试剂盒
  • 货号:
    CSB-E08710m
  • 规格:
    96T/48T
  • 价格:
    ¥3200/¥2500
  • 其他:

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  • 产品描述:
    CUSABIO小鼠末端补体复合物C5b-9(TCC C5b-9)酶联免疫试剂盒(货号:CSB-E08710m),采用夹心法原理实现定量检测,适用于评估补体系统终末途径激活后形成的膜攻击复合物C5b-9水平。该复合物通过介导细胞膜穿孔参与免疫防御及炎症反应调控,在免疫相关疾病机制研究中具有重要价值。试剂盒检测范围覆盖0.78 ng/mL至50 ng/mL,可精准分析血清、血浆、细胞培养上清及组织匀浆等多种样本类型。通过预包被特异性抗体的微孔板捕获目标蛋白,结合酶标抗体形成复合物后显色,实现浓度测定。该产品适用于科研场景中炎症性疾病模型评估、补体通路功能研究或药物干预效果分析,为探索免疫调节机制提供可靠工具,所有应用仅限于实验室研究。
  • 缩写:
    TCC C5b9
  • 种属:
    Mus musculus (Mouse)
  • 样本类型:
    serum, plasma, cell culture supernates, tissue homogenates
  • 检测范围:
    0.78 ng/mL-50 ng/mL
  • 灵敏度:
    0.195 ng/mL
  • 反应时间:
    1-5h
  • 样本体积:
    50-100ul
  • 检测波长:
    450 nm
  • 研究领域:
    Others
  • 测定原理:
    quantitative
  • 测定方法:
    Sandwich
  • 精密度:
    Intra-assay Precision (Precision within an assay): CV%<8%
    Three samples of known concentration were tested twenty times on one plate to assess.
    Inter-assay Precision (Precision between assays): CV%<10%
    Three samples of known concentration were tested in twenty assays to assess.
  • 线性度:
    To assess the linearity of the assay, samples were spiked with high concentrations of mouse TCC C5b-9 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
     SampleSerum(n=4)
    1:100Average %88
    Range %84-96
    1:200Average %98
    Range %91-105
    1:400Average %95
    Range %91-99
    1:800Average %92
    Range %88-96
  • 回收率:
    The recovery of mouse TCC C5b-9 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.
    Sample TypeAverage % RecoveryRange
    Serum (n=5) 9287-97
    EDTA plasma (n=4)9792-105
  • 标准曲线:
    These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.
    ng/mlOD1OD2AverageCorrected
    502.684 2.810 2.747 2.596
    252.054 2.212 2.133 1.982
    12.51.426 1.446 1.436 1.285
    6.250.854 0.867 0.861 0.710
    3.120.622 0.585 0.604 0.453
    1.560.337 0.350 0.344 0.193
    0.780.258 0.264 0.261 0.110
    00.148 0.154 0.151  
  • 数据处理:
  • 货期:
    3-5 working days

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相关问答

 常见问题解答
Q:

您的试剂盒是否可以检测小鼠体内的可溶性C5b-C9?

A:
该试剂盒可检测可溶性TCC C5b-9。“C5b-C9”目前不使用。