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  4. Mouse serum amyloid A2 (SAA2) ELISA kit

Mouse serum amyloid A2 (SAA2) ELISA kit

说明书下载
  • 中文名称：
    小鼠血清淀粉样蛋白A2(SAA2)酶联免疫试剂盒
  • 货号：
    CSB-EL020657MO
  • 规格：
    96T/48T
  • 价格：
    ￥3600/￥2500
  • 其他：
CUSABIO Wins 2024 Bioz Rapid Star™ Award in the ELISA category

产品详情

  • 产品描述：
    小鼠血清淀粉样蛋白A2(SAA2)酶联免疫试剂盒（CSB-EL020657MO）为双抗夹心法ELISA试剂盒，定量检测血清、血浆、组织匀浆样本中的SAA2含量。SAA2即血清淀粉样蛋白A2，它与炎症反应密切相关。在机体受到感染、损伤等刺激时表达上调。研究机制方面，主要聚焦于其在炎症信号传导通路中的作用，以及对免疫细胞的调节，探索其作为炎症相关疾病诊断标志物和治疗靶点的潜力。试剂盒检测范围为7.8 ng/mL-500 ng/mL，适用于基础科研中炎症机制研究、疾病模型动态监测或代谢相关实验的生物学标记分析。尤其适合体外实验如细胞因子诱导模型、感染性疾病动物模型的血清或组织样本检测，为炎症相关信号通路研究提供可靠工具。本品仅用于科研，不用于临床诊断，产品具体参数及操作步骤详见产品说明书。
  • 别名：
    Saa2 ELISA Kit; Serum amyloid A-2 protein [Cleaved into: Amyloid protein A ELISA Kit; Amyloid fibril protein AA)] ELISA Kit
  • 缩写：
    SAA2
  • Uniprot No.：
  • 种属：
    Mus musculus (Mouse)
  • 样本类型：
    serum, plasma, tissue homogenates
  • 检测范围：
    7.8 ng/mL-500 ng/mL
  • 灵敏度：
    1.95 ng/mL
  • 反应时间：
    1-5h
  • 样本体积：
    50-100ul
  • 检测波长：
    450 nm
  • 研究领域：
    Cardiovascular
  • 测定原理：
    quantitative
  • 测定方法：
    Sandwich
  • 精密度：
    Intra-assay Precision (Precision within an assay): CV%<8%
    Three samples of known concentration were tested twenty times on one plate to assess.
    Inter-assay Precision (Precision between assays): CV%<10%
    Three samples of known concentration were tested in twenty assays to assess.
  • 线性度：
    To assess the linearity of the assay, samples were spiked with high concentrations of mouse SAA2 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
    　 Sample Serum(n=4)
    1:1 Average % 108
    Range % 98-112
    1:2 Average % 97
    Range % 88-101
    1:4 Average % 90
    Range % 83-94
    1:8 Average % 100
    Range % 90-104
  • 回收率：
    The recovery of mouse SAA2 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.
    Sample Type Average % Recovery Range
    Serum (n=5) 102 93-106
    EDTA plasma (n=4) 93 85-97
  • 标准曲线：
    These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.
    ng/ml OD1 OD2 Average Corrected
    500 1.723 1.835 1.779 1.591
    250 1.277 1.189 1.233 1.045
    125 0.864 0.887 0.876 0.688
    62.5 0.685 0.674 0.680 0.492
    31.2 0.463 0.456 0.460 0.272
    15.6 0.384 0.367 0.376 0.188
    7.8 0.286 0.291 0.289 0.101
    0 0.190 0.186 0.188 　
  • 数据处理：
    ELISA操作视频及标曲绘制软件下载
  • 货期：
    3-5 working days

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Saa2 Proteins

靶点详情

  • 功能：
    Major acute phase reactant.
  • 基因功能参考文献：
    1. High level SAA expression induced amyloidosis in all mice after a short, slightly variable delay. PMID: 23959890
    2. These results suggest that the carboxy terminus of SAA, which is highly conserved among SAA sequences in all vertebrates, might play important structural roles, including modulating the folding, oligomerization, misfolding, and fibrillation of SAA.(Saa2) PMID: 22448726
    3. The nonpathogenic murine Saa2.2 spontaneously forms marginally stable amyloid fibrils at 37 degrees C that exhibit cross-beta structure, binding to thioflavin T, and fibrillation by a nucleation-dependent seeding mechanism. PMID: 21942925
    4. The ability of SAA2.2 to form different oligomeric species in vitro along with its marginal stability, suggest that the structure of SAA might be modulated in vivo to form different biologically relevant species. PMID: 21439938
    5. forms a hexamer containing a central channel in solution PMID: 12456883
    6. Only liposomes containing intact SAA2.1 or its residues 1-20 or 74-103 promoted the efflux of cholesterol in vivo PMID: 12951366
    7. SAA2.2 can form amyloid fibrils in vitro at physiological temperatures, which suggests that SAA2.2's inability to cause amyloidosis may be related to the stabilization of hexameric SAA2.2 and/or the slow kinetics of aberrant misfolding and self-assembly. PMID: 16194868
    8. Many functional and pathological roles attributed to serum amyloid A may rely on its precarious structure, modulated by its interaction with ligands under homeostasis conditions and during the acute phase response. PMID: 17425332

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  • 相关疾病：
    Reactive, secondary amyloidosis is characterized by the extracellular accumulation in various tissues of the SAA protein. These deposits are highly insoluble and resistant to proteolysis; they disrupt tissue structure and compromise function.
  • 亚细胞定位：
    Secreted.
  • 蛋白家族：
    SAA family
  • 组织特异性：
    Expressed by the liver; secreted in plasma.
  • 数据库链接：

    KEGG: mmu:20209

    STRING: 10090.ENSMUSP00000075365

    UniGene: Mm.200941

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九游平台-九游(中国) | 米兰体育-米兰体育（中国） | 开云手机在线登陆入口 | 江南官方体育网页版 | 开云网页版 | 开云官方在线入口 | 开云电子体育 | 开云官方链接 | 江南网页版页面登录 |