Mouse pro interleukin 1 beta (pro-IL1B) ELISA Kit

产品详情

产品描述：

CUSABIO小鼠白介素1β前体（pro-IL1B）定量检测试剂盒（货号CSB-ET011614MO），基于双抗体夹心法原理开发，可精准检测血清、血浆、细胞培养上清液及组织匀浆样本中的pro-IL1B蛋白水平。pro-IL1B是白介素1β的无活性前体蛋白，主要在巨噬细胞等免疫细胞中表达，经炎症信号激活后通过酶切形成成熟的IL-1β，在机体免疫应答和炎症反应调控中发挥关键作用。本试剂盒检测灵敏度高，线性范围为6.25-400 pg/mL，可兼容多种复杂样本类型，适用于体外炎症模型构建、免疫调控机制研究、药物抗炎效果评估等科研领域。实验操作采用预包被特异性捕获抗体的96孔板，配合检测抗体及显色系统完成定量分析，具有高效稳定的检测性能，为炎症相关基础研究提供可靠工具。
缩写：

pro-IL1B
种属：

Mus musculus (Mouse)
样本类型：

serum, plasma, cell culture supernates, tissue homogenates
检测范围：

6.25 pg/mL-400 pg/mL
灵敏度：

1.56 pg/mL
反应时间：

1-5h
样本体积：

50-100ul
检测波长：

450 nm
研究领域：

Immunology
测定原理：

quantitative
测定方法：

Sandwich

精密度：

Intra-assay Precision (Precision within an assay): CV%<8%
Three samples of known concentration were tested twenty times on one plate to assess.
Inter-assay Precision (Precision between assays): CV%<10%
Three samples of known concentration were tested in twenty assays to assess.

线性度：

To assess the linearity of the assay, samples were spiked with high concentrations of mouse pro-IL1B in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
	Sample	Serum(n=4)
1:1	Average %	106
	Range %	101-113
1:2	Average %	87
	Range %	82-91
1:4	Average %	94
	Range %	90-99
1:8	Average %	85
	Range %	80-90

回收率：

The recovery of mouse pro-IL1B spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.

Sample Type	Average % Recovery	Range
Serum (n=5)	96	91-100
EDTA plasma (n=4)	91	85-97

标准曲线：

These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.