Mouse plasminogen activator inhibitor 1,PAI-1 ELISA Kit

Mouse plasminogen activator inhibitor 1,PAI-1 ELISA Kit

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中文名称：

小鼠纤溶酶原激活物抑制因子1(PAI-1)酶联免疫试剂盒
货号：

CSB-E07947m
规格：

96T/48T
价格：

￥3800/￥2500
其他：

CUSABIO Wins 2024 Bioz Rapid Star™ Award in the ELISA category

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产品描述：

本试剂盒（货号：CSB-E07947m）采用双抗体夹心法定量检测小鼠血清、血浆及组织匀浆中的纤溶酶原激活物抑制因子1（PAI-1），该靶标作为纤溶系统关键调控因子，在抑制纤维蛋白溶解、调控血栓形成及参与组织修复过程中发挥重要作用。试剂盒检测灵敏度为0.9 ng/mL，线性范围覆盖0.9-60 ng/mL，可在4.5小时内完成样本检测，适用于心血管疾病模型、血栓形成机制研究以及创伤后组织修复等科研领域。实验采用预包被特异性抗体的96孔板，通过标准品梯度稀释建立标准曲线，配合HRP标记检测抗体进行显色反应，可稳定检测多种生物样本中PAI-1的浓度变化。该试剂盒提供完整配套试剂及操作指南，支持科研人员精准分析PAI-1在病理生理过程中的动态表达，为纤溶系统相关分子机制研究提供可靠工具。
别名：

Serpine1 ELISA Kit; Mr1 ELISA Kit; Pai1 ELISA Kit; Planh1 ELISA Kit; Plasminogen activator inhibitor 1 ELISA Kit; PAI ELISA Kit; PAI-1 ELISA Kit; Endothelial plasminogen activator inhibitor ELISA Kit; Serpin E1 ELISA Kit
缩写：

SERPINE1
Uniprot No.：

P22777
种属：

Mus musculus (Mouse)
样本类型：

serum, plasma, tissue homogenates
检测范围：

0.9 ng/mL-60 ng/mL
灵敏度：

0.22 ng/mL
反应时间：

1-5h
样本体积：

50-100ul
检测波长：

450 nm
研究领域：

Cell Biology
测定原理：

quantitative
测定方法：

Sandwich

精密度：

线性度：

To assess the linearity of the assay, samples were spiked with high concentrations of mouse PAI-1 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
	Sample	Serum(n=4)
1:1	Average %	92
	Range %	88-95
1:2	Average %	95
	Range %	90-100
1:4	Average %	94
	Range %	88-102
1:8	Average %	96
	Range %	91-103

回收率：

The recovery of mouse PAI-1 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.

Sample Type	Average % Recovery	Range
Serum (n=5)	95	89-101
EDTA plasma (n=4)	95	92-103

标准曲线：

These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

ng/ml	OD1	OD2	Average	Corrected
60	3.033	3.108	3.071	2.894
30	2.476	2.557	2.517	2.340
15	1.887	1.785	1.836	1.659
7.5	1.117	1.124	1.121	0.944
3.75	0.711	0.719	0.715	0.538
1.8	0.488	0.468	0.478	0.301
0.9	0.329	0.332	0.331	0.154
0	0.178	0.176	0.177

数据处理：

ELISA操作视频及标曲绘制软件下载
货期：

3-5 working days

The Astragaloside IV Derivative LS-102 Ameliorates Obesity-Related Nephropathy Z Li,Development and Therapy,2023
Peroxisome proliferator-activated receptor α attenuates high-cholesterol diet-induced toxicity and pro-thrombotic effects in mice Lu Y.et al,archives of toxicology,2018

功能：

Serine protease inhibitor. Inhibits TMPRSS7. Is a primary inhibitor of tissue-type plasminogen activator (PLAT) and urokinase-type plasminogen activator (PLAU). As PLAT inhibitor, it is required for fibrinolysis down-regulation and is responsible for the controlled degradation of blood clots. As PLAU inhibitor, it is involved in the regulation of cell adhesion and spreading. Acts as a regulator of cell migration, independently of its role as protease inhibitor. It is required for stimulation of keratinocyte migration during cutaneous injury repair. Involved in cellular and replicative senescence. Plays a role in alveolar type 2 cells senescence in the lung. Is involved in the regulation of cementogenic differentiation of periodontal ligament stem cells, and regulates odontoblast differentiation and dentin formation during odontogenesis.
亚细胞定位：

Secreted.
蛋白家族：

Serpin family
数据库链接：

STRING: 10090.ENSMUSP00000039586

UniGene: Mm.250422

Intra-assay Precision (Precision within an assay): CV%<8%

Three samples of known concentration were tested twenty times on one plate to assess.

Inter-assay Precision (Precision between assays): CV%<10%

Three samples of known concentration were tested in twenty assays to assess.