Mouse plasma kallikrein(KLKB1) ELISA Kit

Mouse plasma kallikrein(KLKB1) ELISA Kit

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中文名称：

小鼠血浆激肽释放酶(KLKB1)酶联免疫试剂盒
货号：

CSB-E16637m
规格：

96T/48T
价格：

￥3600/￥2500
其他：

CUSABIO Wins 2024 Bioz Rapid Star™ Award in the ELISA category

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CUSABIO小鼠血浆激肽释放酶（KLKB1）酶联免疫检测试剂盒（货号：CSB-E16637m），采用双抗体夹心法原理实现样本中KLKB1蛋白的定量分析，适用于血清、血浆及组织匀浆样本检测，检测灵敏度达3.12 ng/mL，线性范围为3.12 ng/mL至200 ng/mL。KLKB1是激肽释放酶-激肽系统（KKS）的关键酶，通过催化激肽原生成激肽参与凝血调控、炎症反应及血压调节等生理过程，其表达水平与心血管疾病、代谢紊乱等研究密切相关。试剂盒包含预包被捕获抗体的96孔板、检测抗体及标准品，操作时通过生物素-链霉亲和素系统放大信号，可在4.5小时内完成检测流程，标准品呈梯度浓度配置便于绘制标准曲线。本产品兼容多类型样本，支持基础科研中KLKB1相关机制研究，例如炎症模型中的激肽系统动态分析、药物干预对凝血-纤溶平衡的影响评估，或代谢性疾病动物模型中酶活性变化的探索。试剂盒组分需在-20°C避光保存，有效期12个月，建议实验前通过预实验优化样本稀释比例以确保检测值位于线性范围内。
别名：

Klkb1 ELISA Kit; Klk3 ELISA Kit; PkPlasma kallikrein ELISA Kit; EC 3.4.21.34 ELISA Kit; Fletcher factor ELISA Kit; Kininogenin ELISA Kit; Plasma prekallikrein) [Cleaved into: Plasma kallikrein heavy chain; Plasma kallikrein light chain] ELISA Kit
缩写：

KLKB1
Uniprot No.：

P26262
种属：

Mus musculus (Mouse)
样本类型：

serum, plasma, tissue homogenates
检测范围：

3.12 ng/mL-200 ng/mL
灵敏度：

0.78 ng/mL
反应时间：

1-5h
样本体积：

50-100ul
检测波长：

450 nm
研究领域：

Cell Biology
测定原理：

quantitative
测定方法：

Sandwich

精密度：

线性度：

To assess the linearity of the assay, samples were spiked with high concentrations of mouse KLKB1 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
	Sample	Serum(n=4)
1:1000	Average %	99
	Range %	96-102
1:2000	Average %	103
	Range %	100-106
1:4000	Average %	89
	Range %	84-94
1:8000	Average %	96
	Range %	94-98

回收率：

The recovery of mouse KLKB1 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.

Sample Type	Average % Recovery	Range
Serum (n=5)	93	89-97
EDTA plasma (n=4)	103	100-105

标准曲线：

These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

ng/ml	OD1	OD2	Average	Corrected
200	2.459	2.555	2.507	2.388
100	1.877	1.966	1.922	1.803
50	1.355	1.332	1.344	1.225
25	0.789	0.768	0.779	0.660
12.5	0.546	0.588	0.567	0.448
6.25	0.323	0.345	0.334	0.215
3.12	0.204	0.221	0.213	0.094
0	0.118	0.119	0.119

数据处理：

ELISA操作视频及标曲绘制软件下载
货期：

3-5 working days

Inhibition of plasma kallikrein mitigates experimental hypertension-enhanced cerebral hematoma expansion J Wang,Brain Research Bulletin,2021

功能：

The enzyme cleaves Lys-Arg and Arg-Ser bonds. It activates, in a reciprocal reaction, factor XII after its binding to a negatively charged surface. It also releases bradykinin from HMW kininogen and may also play a role in the renin-angiotensin system by converting prorenin into renin.
基因功能参考文献：
1. Plasma kallikrein mediates brain hemorrhage and edema caused by tissue plasminogen activator therapy in mice after stroke. PMID: 28130211
2. we found that post-ejaculated semen from fertile wild-type males was solidified and the sperm were entrapped in Wnt7aCre/+;Esr1f/f uteri, compared to the watery semen (liquefied) found in Esr1f/f controls.Kallikrein-related peptidases 3 (KLK3) and other kallikrein-related peptidases from male prostate glands are responsible for semen liquefaction by cleaving gel-forming proteins PMID: 28414719
3. Plasma kallikrein deletion prevents occlusive thrombus formation in mice with a minimal role in provoked bleeding. PMID: 22398951
4. PKK or fXII deficiency reduced thrombus formation in both arterial and venous thrombosis models, without an apparent effect on hemostasis. PMID: 21821705
5. the prekallikrein gene (Klkb1) is expressed highly in the mammary gland during stromal remodeling periods including puberty and postlactational involution. PMID: 19297327
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亚细胞定位：

Secreted.
蛋白家族：

Peptidase S1 family, Plasma kallikrein subfamily
数据库链接：

KEGG: mmu:16621

STRING: 10090.ENSMUSP00000112174

UniGene: Mm.482691

Intra-assay Precision (Precision within an assay): CV%<8%

Three samples of known concentration were tested twenty times on one plate to assess.

Inter-assay Precision (Precision between assays): CV%<10%

Three samples of known concentration were tested in twenty assays to assess.