Mouse oxyntomodulin ELISA Kit

产品详情

Intra-assay Precision (Precision within an assay): CV%<8%

Three samples of known concentration were tested twenty times on one plate to assess.

Inter-assay Precision (Precision between assays): CV%<10%

Three samples of known concentration were tested in twenty assays to assess.

产品描述：

小鼠胃泌酸调节素酶联免疫试剂盒（CSB-E12950m）为双抗夹心法ELISA试剂盒，定量检测血清、血浆、组织培养上清液、组织匀浆样本中的oxyntomodulin含量。小鼠胃泌酸调节素（OXM）是一种由胃内分泌细胞分泌的肽激素，主要作用是调节胃酸分泌和胃肠道功能。其研究机制涉及对胃肠激素、肠道菌群、胃肠道神经递质、脑-肠肽、Cajal间质细胞和胃肠电活动的调节，以及苦味受体和离子通道的影响。试剂盒检测范围为0.312 pg/mL-20 pg/mL，适用于胃肠生理机制、药物干预效果评估或相关疾病模型的体外研究。本品仅用于科研，不用于临床诊断，产品具体参数及操作步骤详见产品说明书。
缩写：

oxyntomodulin
种属：

Mus musculus (Mouse)
样本类型：

serum, plasma, cell culture supernates, tissue homogenates
检测范围：

0.312 pg/mL-20 pg/mL
灵敏度：

0.078 pg/mL
反应时间：

1-5h
样本体积：

50-100ul
检测波长：

450 nm
研究领域：

Metabolism
测定原理：

quantitative
测定方法：

Sandwich

精密度：

线性度：

To assess the linearity of the assay, samples were spiked with high concentrations of mouse oxyntomodulin in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
	Sample	Serum(n=4)
1:5	Average %	102
	Range %	92-108
1:10	Average %	89
	Range %	85-96
1:20	Average %	97
	Range %	91-105
1:40	Average %	92
	Range %	87-96

回收率：

The recovery of mouse oxyntomodulin spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.

Sample Type	Average % Recovery	Range
Serum (n=5)	95	89-99
EDTA plasma (n=4)	91	86-97

标准曲线：

These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.