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  4. Mouse neuregulin-4 (NRG4) ELISA kit

Mouse neuregulin-4 (NRG4) ELISA kit

文献引用 (1) 说明书下载
  • 中文名称：
    小鼠神经调节蛋白4(NRG4)酶联免疫试剂盒
  • 货号：
    CSB-EL016080MO
  • 规格：
    96T/48T
  • 价格：
    ￥3600/￥2500
  • 其他：
CUSABIO Wins 2024 Bioz Rapid Star™ Award in the ELISA category

产品详情

  • 产品描述：
    CUSABIO小鼠神经调节蛋白4(NRG4)酶联免疫检测试剂盒（货号CSB-EL016080MO），采用双抗体夹心法定量检测血清、血浆或组织匀浆样本中的NRG4蛋白。NRG4作为表皮生长因子家族成员，在调节能量代谢、脂肪组织功能及糖脂稳态中发挥重要作用，其表达水平与肥胖、糖尿病等代谢性疾病的研究密切相关。试剂盒检测灵敏度为3.12 ng/mL，线性范围覆盖3.12-200 ng/mL，可精准测定低丰度样本中NRG4的浓度变化。实验操作基于预包被特异性抗体的96孔板，通过标准品梯度稀释建立标准曲线，结合HRP标记检测抗体及TMB显色系统实现目标蛋白定量分析。该试剂盒适用于科研领域中小鼠模型的基础代谢研究、脂肪组织功能评估或相关疾病机制探索，为体外实验提供高效可靠的工具。试剂盒包含预包被微孔板、标准品、检测抗体及全套实验所需缓冲液组分，支持直接开展实验并获取重复性良好的检测结果。
  • 别名：
    Nrg4 ELISA Kit; Pro-neuregulin-4 ELISA Kit; membrane-bound isoform ELISA Kit; Pro-NRG4) [Cleaved into: Neuregulin-4 ELISA Kit; NRG-4)] ELISA Kit
  • 缩写：
    NRG4
  • Uniprot No.：
  • 种属：
    Mus musculus (Mouse)
  • 样本类型：
    serum, plasma, tissue homogenates
  • 检测范围：
    3.12 ng/mL-200 ng/mL
  • 灵敏度：
    0.78 ng/mL
  • 反应时间：
    1-5h
  • 样本体积：
    50-100ul
  • 检测波长：
    450 nm
  • 研究领域：
    Neuroscience
  • 测定原理：
    quantitative
  • 测定方法：
    Sandwich
  • 精密度：
    Intra-assay Precision (Precision within an assay): CV%<8%
    Three samples of known concentration were tested twenty times on one plate to assess.
    Inter-assay Precision (Precision between assays): CV%<10%
    Three samples of known concentration were tested in twenty assays to assess.
  • 线性度：
    To assess the linearity of the assay, samples were spiked with high concentrations of mouse NRG4 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
    　 Sample Serum(n=4)
    1:1 Average % 97
    Range % 92-103
    1:2 Average % 91
    Range % 84-96
    1:4 Average % 90
    Range % 87-95
    1:8 Average % 100
    Range % 95-105
  • 回收率：
    The recovery of mouse NRG4 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.
    Sample Type Average % Recovery Range
    Serum (n=5) 90 87-93
    EDTA plasma (n=4) 94 90-98
  • 标准曲线：
    These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.
    ng/ml OD1 OD2 Average Corrected
    200 2.549 2.763 2.656 2.556
    100 2.135 2.131 2.133 2.033
    50 1.587 1.622 1.605 1.505
    25 1.014 1.033 1.024 0.924
    12.5 0.736 0.787 0.762 0.662
    6.25 0.468 0.436 0.452 0.352
    3.12 0.214 0.231 0.223 0.123
    0 0.101 0.098 0.100 　
  • 数据处理：
    ELISA操作视频及标曲绘制软件下载
  • 货期：
    3-5 working days

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Nrg4 Proteins

靶点详情

  • 功能：
    Low affinity ligand for the ERBB4 tyrosine kinase receptor. Concomitantly recruits ERBB1 and ERBB2 coreceptors, resulting in ligand-stimulated tyrosine phosphorylation and activation of the ERBB receptors. Does not bind to the ERBB1, ERBB2 and ERBB3 receptors.
  • 基因功能参考文献：
    1. The found of this study showed that NRG4 is a major novel regulator of dendritic arborisation in the developing cerebral cortex and suggest that it exerts its effects by an autocrine/paracrine mechanism. PMID: 29317193
    2. Conditional knockout of Nrg4 in brown adipocytes caused blood vessel reduction in brown but not in white adipose tissue , and was sufficient to induce obese phenotype. PMID: 29902456
    3. Nrg4 exerts beneficial effects on energy balance and glucose and lipid metabolism to ameliorate obesity-associated metabolic disorders. PMID: 28752050
    4. overexpression of the Nrg4 gene by hydrodynamic gene delivery prevents high fat diet-induced weight gain and fatty liver, alleviates obesity-induced chronic inflammation and insulin resistance PMID: 27184920
    5. Pharmacological inhibition of PI3K/Akt signaling reversed the anti-apoptotic effects of NRG4, confirming the role of this cascade in NRG4-induced cell survival PMID: 23033483

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  • 亚细胞定位：
    [Pro-neuregulin-4, membrane-bound isoform]: Cell membrane; Single-pass type I membrane protein.; [Neuregulin-4]: Secreted.
  • 蛋白家族：
    Neuregulin family
  • 组织特异性：
    Highly expressed in pancreas; weakly expressed in muscle.
  • 数据库链接：

    KEGG: mmu:83961

    STRING: 10090.ENSMUSP00000115247

    UniGene: Mm.443874

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九游平台-九游(中国) | 米兰体育-米兰体育（中国） | 开云手机在线登陆入口 | 江南官方体育网页版 | 开云网页版 | 开云官方在线入口 | 开云电子体育 | 开云官方链接 | 江南网页版页面登录 |