Mouse myeloperoxidase-antineutrophil cytoplasmic antibody ELISA kit

产品详情

Intra-assay Precision (Precision within an assay): CV%<8%

Three samples of known concentration were tested twenty times on one plate to assess.

Inter-assay Precision (Precision between assays): CV%<10%

Three samples of known concentration were tested in twenty assays to assess.

产品描述：

本产品是一款专用于定量检测小鼠血清或血浆样本中髓过氧化物酶特异性抗中性粒细胞胞质抗体（MPO-ANCA）的ELISA试剂盒。髓过氧化物酶（MPO）是中性粒细胞颗粒中的关键酶，参与宿主防御反应，其自身抗体MPO-ANCA与多种自身免疫性疾病病理机制相关。该试剂盒基于双抗体夹心ELISA技术开发，检测灵敏度高，线性范围为0.625-40 ng/mL，可精准测定低浓度至中高浓度目标抗体水平。实验操作采用预包被特异性捕获抗体的96孔板，通过标准品与样本中MPO-ANCA的竞争结合实现定量分析，适用于科研领域探究小鼠模型中MPO-ANCA的动态变化，如评估炎症性疾病进展、自身免疫模型构建或药物干预效果研究。试剂盒兼容血清、血浆样本类型，配套提供标准品、检测抗体及显色底物，支持科研人员在体外实验中高效获取稳定可靠的实验数据。
缩写：

MAC Ab
种属：

Mus musculus (Mouse)
样本类型：

serum, plasma
检测范围：

0.625 ng/mL-40 ng/mL
灵敏度：

0.156 ng/mL
反应时间：

1-5h
样本体积：

50-100ul
检测波长：

450 nm
研究领域：

Others
测定原理：

quantitative
测定方法：

Sandwich

精密度：

线性度：

To assess the linearity of the assay, samples were spiked with high concentrations of mouse myeloperoxidase-antineutrophil cytoplasmic antibody (MPO-ANCA)in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
	Sample	Serum(n=4)
1:1	Average %	95
	Range %	90-102
1:2	Average %	95
	Range %	83-101
1:4	Average %	96
	Range %	90-106
1:8	Average %	94
	Range %	82-101

回收率：

The recovery of mouse myeloperoxidase-antineutrophil cytoplasmic antibody(MPO-ANCA)spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.


Sample Type	Average % Recovery	Range
Serum (n=5)	92	89-98
EDTA plasma (n=4)	94	91-100

标准曲线：

These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.