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  4. Mouse monocyte chemotactic protein 2,MCP-2 ELISA kit

Mouse monocyte chemotactic protein 2,MCP-2 ELISA kit

文献引用 (2) 说明书下载
  • 中文名称：
    小鼠单核细胞趋化蛋白2(MCP-2/CCL8)酶联免疫试剂盒
  • 货号：
    CSB-E07428m
  • 规格：
    96T/48T
  • 价格：
    ￥3200/￥2500
  • 其他：
CUSABIO Wins 2024 Bioz Rapid Star™ Award in the ELISA category

产品详情

  • 产品描述：
    CUSABIO专用于科研领域的小鼠单核细胞趋化蛋白2（MCP-2/CCL8）酶联免疫检测试剂盒（货号：CSB-E07428m），该试剂盒基于双抗体夹心法原理设计，可对血清、血浆及组织匀浆样本中的MCP-2/CCL8进行定量检测，其检测灵敏度达6.25 pg/mL，线性范围为6.25-400 pg/mL。MCP-2/CCL8属于CC趋化因子家族成员，在炎症反应、免疫细胞迁移及肿瘤微环境调控中发挥重要作用，其表达水平与多种病理过程相关。本试剂盒通过高特异性抗体对实现精准捕获和信号放大，适用于小鼠模型中的炎症机制研究、免疫调控实验或相关疾病（如自身免疫病、感染性疾病）的分子标志物分析，能够稳定检测生物样本中低至皮克级别的目标蛋白含量，为科研人员探索趋化因子网络功能提供可靠工具。实验操作全程无需特殊设备，兼容常规酶标仪读取结果，建议在标准实验室条件下按照说明书流程开展研究。
  • 别名：
    Ccl8 ELISA Kit; Mcp2 ELISA Kit; Scya8C-C motif chemokine 8 ELISA Kit; Monocyte chemoattractant protein 2 ELISA Kit; Monocyte chemotactic protein 2 ELISA Kit; MCP-2 ELISA Kit; Small-inducible cytokine A8 ELISA Kit
  • 缩写：
    CCL8
  • Uniprot No.：
  • 种属：
    Mus musculus (Mouse)
  • 样本类型：
    serum, plasma, tissue homogenates
  • 检测范围：
    6.25 pg/mL-400 pg/mL
  • 灵敏度：
    1.56 pg/mL
  • 反应时间：
    1-5h
  • 样本体积：
    50-100ul
  • 检测波长：
    450 nm
  • 研究领域：
    Immunology
  • 测定原理：
    quantitative
  • 测定方法：
    Sandwich
  • 精密度：
    Intra-assay Precision (Precision within an assay): CV%<8%
    Three samples of known concentration were tested twenty times on one plate to assess.
    Inter-assay Precision (Precision between assays): CV%<10%
    Three samples of known concentration were tested in twenty assays to assess.
  • 线性度：
    To assess the linearity of the assay, samples were spiked with high concentrations of mouse MCP-2 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
    SampleSerum(n=4)
    1:1Average %87
    Range %85-88
    1:2Average %102
    Range %98-106
    1:4Average %98
    Range %96-101
    1:8Average %92
    Range %89-95
  • 回收率：
    The recovery of mouse MCP-2 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.
    Sample TypeAverage % RecoveryRange
    Serum (n=5) 8884-92
    EDTA plasma (n=4)9592-98
  • 标准曲线：
    These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.
    pg/mlOD1OD2AverageCorrected
    4002.955 2.781 2.868 2.720
    2002.138 2.156 2.147 1.999
    1001.445 1.532 1.489 1.341
    500.921 0.903 0.912 0.764
    250.584 0.561 0.573 0.425
    12.50.333 0.324 0.329 0.181
    6.250.241 0.253 0.247 0.099
    00.142 0.153 0.148
  • 数据处理：
    ELISA操作视频及标曲绘制软件下载
  • 货期：
    3-5 working days

引用文献

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Ccl8 Proteins

靶点详情

  • 功能：
    Chemotactic factor that attracts monocytes. This protein can bind heparin.
  • 基因功能参考文献：
    1. Findings exemplify how gradients of chemoattractive factors such as CCL8, drive metastasis and suggest that interference with their operation may provide means for breast cancer management. PMID: 27181207
    2. these data reveal an important role for BLIMP1 in modulating host defenses by suppressing expression of the chemokine CCL8. PMID: 24477914
    3. Results indicate that the induction of MCP-2/CCL8 by mycobacteria is dependent on the activation of TLR2/PI3K/Akt signaling pathway. PMID: 23418602
    4. Vitamin B6 supplementation downregulates Cd8a and Ccl8 mRNA expression, so these candidate genes may play a protective role against colonic aberrant crypt foci development. PMID: 22495988
    5. early and preclinical expression of CCL8 in plasma predicts overall survival of graft-vs.-host disease (GVHD) mice. Together with an involvement of allo-recognition in CCL8 expression, it suggests that CCL8 plays an important role in GVHD pathology. PMID: 21782767
    6. MCP/CCL8 was induced via STAT3 and exhibited anti-tumor metastatic effect PMID: 21624767
    7. promotes atopic dermatitis by recruiting IL-5+ Th2 cells PMID: 21217759
    8. CCL8 proteins were present in the vast majority of tissues investigated. mRNA for these proteins was also expressed in most of these tissues suggesting local production and the ability to respond in situ to inflammatory stimuli. PMID: 20931267
    9. Data show that that the stromal cell-derived chemokines CXCL12 and CCL8 cooperate to attract hematopoietic progenitors with the potential to differentiate into regulatory DCs. PMID: 20624948
    10. CCL8 is a promising specific serum marker for the early and accurate diagnosis of graft-versus-host disease. PMID: 18256320
    11. An elevated plasma CCL8 concentration may be a promising plasma marker for GVHD in mouse models PMID: 19302923

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  • 亚细胞定位：
    Secreted.
  • 蛋白家族：
    Intercrine beta (chemokine CC) family
  • 数据库链接：

    KEGG: mmu:20307

    STRING: 10090.ENSMUSP00000009329

    UniGene: Mm.42029

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九游平台-九游(中国) | 米兰体育-米兰体育（中国） | 开云手机在线登陆入口 | 江南官方体育网页版 | 开云网页版 | 开云官方在线入口 | 开云电子体育 | 开云官方链接 | 江南网页版页面登录 |