Mouse mitochondrial respiratory chain complex I ELISA kit

产品详情

产品描述：

小鼠线粒体呼吸链复合物I酶联免疫试剂盒（CSB-EQ027280MO）为双抗夹心法ELISA试剂盒，定量检测血清、血浆、组织匀浆、细胞裂解物样本中的MRCC-I含量。试剂盒检测范围为7.8 pg/mL-500 pg/mL，本试剂盒采用高特异性配对抗体，通过标准化的预包被板与显色系统实现目标蛋白的定量分析，适用于基础研究中线粒体功能评估、疾病模型构建（如心肌缺血、阿尔茨海默症等）或药物干预后能量代谢变化的动态监测。本品仅用于科研，不用于临床诊断，产品具体参数及操作步骤详见产品说明书。
缩写：

MRCC-I
种属：

Mus musculus (Mouse)
样本类型：

serum, plasma, tissue homogenates, cell lysates
检测范围：

7.8 pg/mL-500 pg/mL
灵敏度：

1.95 pg/mL
反应时间：

1-5h
样本体积：

50-100ul
检测波长：

450 nm
研究领域：

Others
测定原理：

quantitative
测定方法：

Sandwich

精密度：

Intra-assay Precision (Precision within an assay): CV%<8%
Three samples of known concentration were tested twenty times on one plate to assess.
Inter-assay Precision (Precision between assays): CV%<10%
Three samples of known concentration were tested in twenty assays to assess.

线性度：

To assess the linearity of the assay, samples were spiked with high concentrations of mouse MRCC-I in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
	Sample	Serum(n=4)
1:1	Average %	96
	Range %	84-100
1:2	Average %	100
	Range %	94-103
1:4	Average %	94
	Range %	84-98
1:8	Average %	100
	Range %	93-103

回收率：

The recovery of mouse MRCC-I spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.

Sample Type	Average % Recovery	Range
Serum (n=5)	92	84-97
EDTA plasma (n=4)	99	91-103

标准曲线：

These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.