Mouse matrix metallopeptidase 7 (matrilysin, uterine) (MMP7) ELISA kit

Mouse matrix metallopeptidase 7 (matrilysin, uterine) (MMP7) ELISA kit

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中文名称：

小鼠基质金属蛋白酶7(MMP7)酶联免疫试剂盒
货号：

CSB-E07409m
规格：

96T/48T
价格：

￥3200/￥2500
其他：

CUSABIO Wins 2024 Bioz Rapid Star™ Award in the ELISA category

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产品描述：

CUSABIO小鼠基质金属蛋白酶7（MMP7）酶联免疫检测试剂盒（货号CSB-E07409m），采用双抗体夹心法定量检测血清、血浆、细胞培养上清液及组织匀浆中的MMP7含量。MMP7属于基质金属蛋白酶家族成员，通过降解细胞外基质成分参与组织重塑、炎症反应及肿瘤侵袭转移等病理生理过程。试剂盒检测灵敏度达31.25 pg/mL，线性范围为31.25-2000 pg/mL，可在标准曲线范围内精准测定样本浓度。适用于多种科研场景，如肿瘤微环境研究中MMP7的表达水平分析、炎症性疾病模型中的蛋白酶活性评估，或组织修复机制探索实验中细胞分泌动态监测。其宽泛的样本兼容性支持研究人员针对不同实验体系（如体外细胞培养分泌检测、动物模型组织提取物分析等）开展定量研究，为探究MMP7在疾病发生发展中的调控作用提供可靠工具。试剂盒包含预包被捕获抗体、检测抗体及优化反应体系，操作流程标准化，重复性良好。
别名：

Mmp7Matrilysin ELISA Kit; EC 3.4.24.23 ELISA Kit; Matrin ELISA Kit; Matrix metalloproteinase-7 ELISA Kit; MMP-7 ELISA Kit; Pump-1 protease ELISA Kit; Uterine metalloproteinase ELISA Kit
缩写：

MMP7
Uniprot No.：

Q10738
种属：

Mus musculus (Mouse)
样本类型：

serum, plasma, cell culture supernates, tissue homogenates
检测范围：

31.25 pg/mL-2000 pg/mL
灵敏度：

7.81 pg/mL
反应时间：

1-5h
样本体积：

50-100ul
检测波长：

450 nm
研究领域：

Cancer
测定原理：

quantitative
测定方法：

Sandwich

精密度：

线性度：

To assess the linearity of the assay, samples were spiked with high concentrations of mouse MMP7 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
	Sample	Serum(n=4)
1:1	Average %	93
	Range %	90-95
1:2	Average %	102
	Range %	98-104
1:4	Average %	92
	Range %	88-96
1:8	Average %	99
	Range %	95-102

回收率：

The recovery of mouse MMP7 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.

Sample Type	Average % Recovery	Range
Serum (n=5)	94	92-96
EDTA plasma (n=4)	96	92-99

标准曲线：

These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

pg/ml	OD1	OD2	Average	Corrected
2000	2.180	2.088	2.134	1.981
1000	1.548	1.534	1.541	1.388
500	0.882	0.899	0.891	0.738
250	0.511	0.489	0.500	0.347
125	0.338	0.331	0.335	0.182
62.5	0.256	0.248	0.252	0.099
31.25	0.202	0.211	0.207	0.054
0	0.154	0.152	0.153

数据处理：

ELISA操作视频及标曲绘制软件下载
货期：

3-5 working days

Distance-dependent visual fluorescence immunoassay on CdTe quantum dot–impregnated paper through silver ion-exchange reaction L Huang,Microchimica Acta,2020

功能：

Degrades casein, gelatins of types I, III, IV, and V, and fibronectin. Activates procollagenase.; May play a role in tissue reorganization.
亚细胞定位：

Secreted, extracellular space, extracellular matrix.
蛋白家族：

Peptidase M10A family
数据库链接：

STRING: 10090.ENSMUSP00000018767

UniGene: Mm.4825

Intra-assay Precision (Precision within an assay): CV%<8%

Three samples of known concentration were tested twenty times on one plate to assess.

Inter-assay Precision (Precision between assays): CV%<10%

Three samples of known concentration were tested in twenty assays to assess.