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中文名称:小鼠巨噬细胞炎性蛋白3β(MIP-3β/ELC/CCL19)酶联免疫试剂盒
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货号:CSB-E04671m
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规格:96T/48T
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价格:¥3200/¥2500
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其他:
产品详情
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产品描述:CUSABIO小鼠巨噬细胞炎性蛋白3β(MIP-3β/ELC/CCL19)酶联免疫试剂盒(货号:CSB-E04671m),采用夹心法原理实现定量检测,适用于血清、血浆及组织匀浆样本中靶蛋白含量的精准分析。MIP-3β属于CC趋化因子家族成员,通过与CCR7受体结合介导淋巴细胞归巢及树突状细胞迁移,在炎症反应、免疫调控及淋巴组织发育中发挥关键作用,是研究免疫微环境的重要分子标记物。试剂盒检测灵敏度范围为39-2500 pg/ml,可稳定检测样本中痕量至中高浓度的蛋白表达水平,其宽泛的线性范围特别适用于动态监测炎症模型中趋化因子的表达变化。科研人员可通过该试剂盒开展免疫性疾病机制研究,如自身免疫模型、感染性疾病或肿瘤微环境相关实验,尤其适用于评估淋巴结、脾脏等免疫器官中趋化因子的调控规律。该产品严格优化反应体系,提供标准化操作流程,为体外实验提供可靠的数据支持,仅限科学研究使用。
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别名:Ccl19 ELISA Kit; Elc ELISA Kit; Scya19 ELISA Kit; C-C motif chemokine 19 ELISA Kit; Epstein-Barr virus-induced molecule 1 ligand chemokine ELISA Kit; EBI1 ligand chemokine ELISA Kit; ELC ELISA Kit; Small-inducible cytokine A19 ELISA Kit
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缩写:
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Uniprot No.:
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种属:Mus musculus (Mouse)
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样本类型:serum, plasma, tissue homogenates
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检测范围:39 pg/ml - 2500 pg/ml
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灵敏度:9.75 pg/ml
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反应时间:1-5h
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样本体积:50-100ul
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检测波长:450 nm
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研究领域:Immunology
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测定原理:quantitative
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测定方法:Sandwich
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精密度:
Intra-assay Precision (Precision within an assay): CV%<8%
Three samples of known concentration were tested twenty times on one plate to assess.
Inter-assay Precision (Precision between assays): CV%<10%
Three samples of known concentration were tested in twenty assays to assess.
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线性度:
To assess the linearity of the assay, samples were spiked with high concentrations of mouse MIP-3β in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
Sample
Serum(n=4)
1:1
Average %
96
Range %
93-99
1:2
Average %
104
Range %
100-107
1:4
Average %
85
Range %
81-89
1:8
Average %
98
Range %
94-103
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回收率:
The recovery of mouse MIP-3β spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.
Sample Type
Average % Recovery
Range
Serum (n=5)
89
85-94
EDTA plasma (n=4)
108
104-112
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标准曲线:
These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.
pg/ml
OD1
OD2
Average
Corrected
2500
2.830
2.786
2.808
2.629
1250
2.286
2.301
2.294
2.115
625
1.656
1.672
1.664
1.485
312.5
1.007
1.069
1.038
0.859
156.25
0.683
0.698
0.691
0.512
78
0.457
0.438
0.448
0.269
39
0.321
0.331
0.326
0.147
0
0.178
0.179
0.179
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数据处理:
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货期:3-5 working days
引用文献
相关产品
靶点详情
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功能:Strongly chemotactic for naive (L-selectinhi) CD4 T-cells and for CD8 T-cells and weakly attractive for resting B-cells and memory (L-selectinlo) CD4 T-cells. May play a role in promoting encounters between recirculating T-cells and dendritic cells and in the migration of activated B-cells into the T-zone of secondary lymphoid tissues. Binds to chemokine receptor CCR7. Binds to atypical chemokine receptor ACKR4 and mediates the recruitment of beta-arrestin (ARRB1/2) to ACKR4.
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亚细胞定位:Secreted.
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蛋白家族:Intercrine beta (chemokine CC) family
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组织特异性:Highly expressed by dendritic cells in mesenteric and peripheral lymph nodes. Significant expression in spleen (T cell zone or periarteriolar lymphatic sheath) and Peyer patches. Low expression in thymus.
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数据库链接:
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