Mouse intestinal fatty acid binding protein,iFABP ELISA Kit

Mouse intestinal fatty acid binding protein,iFABP ELISA Kit

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中文名称：

小鼠肠脂肪酸结合蛋白(iFABP)酶联免疫试剂盒
货号：

CSB-E08025m
规格：

96T/48T
价格：

￥3200/￥2500
其他：

CUSABIO Wins 2024 Bioz Rapid Star™ Award in the ELISA category

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产品描述：

CUSABIO小鼠肠脂肪酸结合蛋白（iFABP）酶联免疫检测试剂盒（货号：CSB-E08025m），iFABP是肠道特异性表达的脂肪酸转运蛋白，其水平升高与肠道屏障损伤及炎症反应密切相关，常用于科研领域评估肠道功能状态。试剂盒采用双抗体夹心ELISA原理进行定量检测，可分析血清、血浆、细胞培养上清及组织匀浆等多种样本类型，检测灵敏度为6.25 pg/mL，线性范围为6.25-400 pg/mL，满足低丰度靶标检测需求。该试剂盒适用于肠道疾病动物模型研究，例如肠炎、缺血再灌注损伤等病理机制探索，也可用于体外细胞实验中评估药物或外界刺激对肠道上皮细胞的影响，为肠道屏障功能、代谢调控等基础研究提供可靠工具。实验操作全程无需特殊设备，3.5小时内即可完成检测，配套标准品及预包被板条有效保障检测稳定性和重复性。
别名：

Fabp2 ELISA Kit; FabpiFatty acid-binding protein ELISA Kit; intestinal ELISA Kit; Fatty acid-binding protein 2 ELISA Kit; Intestinal-type fatty acid-binding protein ELISA Kit; I-FABP ELISA Kit
缩写：

iFABP
Uniprot No.：

P55050
种属：

Mus musculus (Mouse)
样本类型：

serum, plasma, cell culture supernates, tissue homogenates
检测范围：

6.25 pg/mL-400 pg/mL
灵敏度：

1.56 pg/mL
反应时间：

1-5h
样本体积：

50-100ul
检测波长：

450 nm
研究领域：

Signal Transduction
测定原理：

quantitative
测定方法：

Sandwich

精密度：

线性度：

To assess the linearity of the assay, samples were spiked with high concentrations of mouse iFABP in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
	Sample	Serum(n=4)
1:100	Average %	102
	Range %	96-108
1:200	Average %	95
	Range %	91-99
1:400	Average %	96
	Range %	91-102
1:800	Average %	86
	Range %	82-92

回收率：

The recovery of mouse iFABP spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.

Sample Type	Average % Recovery	Range
Serum (n=5)	88	82-94
EDTA plasma (n=4)	96	90-104

标准曲线：

These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

pg/ml	OD1	OD2	Average	Corrected
400	2.217	2.317	2.267	2.163
200	1.367	1.467	1.417	1.313
100	0.777	0.797	0.787	0.683
50	0.412	0.422	0.417	0.313
25	0.265	0.268	0.267	0.163
12.5	0.189	0.199	0.194	0.090
6.25	0.162	0.165	0.164	0.060
0	0.104	0.103	0.104

数据处理：

ELISA操作视频及标曲绘制软件下载
货期：

3-5 working days

Hesperidin and Fecal Microbiota Transplantation Modulate the Composition of the Gut Microbiota and Reduce Obesity in High Fat Diet Mice T Liu, C Lei, Q Huang, W Song, C Li,Diabetes, Metabolic Syndrome and Obesity,2024
E3 ligase TRIM65 alleviates intestinal ischemia/reperfusion injury through inhibition of TOX4-mediated apoptosis K Daiki,Cell death & disease,2024
Treatment with the Olive Secoiridoid Oleacein Protects against the Intestinal Alterations Associated with EAE B Gutiérrez-Miranda,International journal of molecular sciences,2023
Novel role of hesperidin improve obesity in HFD mice by modulating the composition of the gut microbiota Z Liu,Research Square Preprint,2020

功能：

FABP are thought to play a role in the intracellular transport of long-chain fatty acids and their acyl-CoA esters. FABP2 is probably involved in triglyceride-rich lipoprotein synthesis. Binds saturated long-chain fatty acids with a high affinity, but binds with a lower affinity to unsaturated long-chain fatty acids. FABP2 may also help maintain energy homeostasis by functioning as a lipid sensor.
基因功能参考文献：
1. This direct comparison provides evidence that LFABP and IFABP have distinct roles in intestinal lipid metabolism; differential intracellular functions in intestine and in liver PMID: 23990461
2. detected the common as well as sex-differential pathways that are modified due to the loss of Fabp2. These findings suggest that the pathways involved in nutrient and xenobiotic metabolism in the intestine are regulated by sex-specific mechanisms PMID: 22584290
3. loss of I-FABP renders male mice sensitive to high fat diet-induced fatty liver, and this effect is independent of hepatic L-FABP PMID: 17905650
亚细胞定位：

Cytoplasm.
蛋白家族：

Calycin superfamily, Fatty-acid binding protein (FABP) family
组织特异性：

Expressed in the small intestine. Highest expression levels in the proximal ileum.
数据库链接：

KEGG: mmu:14079

STRING: 10090.ENSMUSP00000023820

UniGene: Mm.28398

Intra-assay Precision (Precision within an assay): CV%<8%

Three samples of known concentration were tested twenty times on one plate to assess.

Inter-assay Precision (Precision between assays): CV%<10%

Three samples of known concentration were tested in twenty assays to assess.