Mouse eosinophil cationic protein,ECP ELISA Kit

产品详情

Intra-assay Precision (Precision within an assay): CV%<8%

Three samples of known concentration were tested twenty times on one plate to assess.

Inter-assay Precision (Precision between assays): CV%<10%

Three samples of known concentration were tested in twenty assays to assess.

产品描述：

CUSABIO试剂盒采用双抗体夹心法定量检测小鼠血清、血浆、细胞培养上清或组织匀浆中的嗜酸性粒细胞阳离子蛋白（ECP），该蛋白是嗜酸性粒细胞活化后释放的毒性颗粒蛋白，参与抗寄生虫免疫及过敏性疾病进程，可作为呼吸道炎症及过敏研究的重要生物标志物。试剂盒检测灵敏度达0.039 ng/mL，在0.156-10 ng/mL范围内呈现优异线性，标准操作流程约需3.5小时，包含孵育、洗涤及显色步骤。96孔板预包被高特异性抗体，配套提供标准品、检测抗体、显色底物及终止液，2-8℃可稳定保存6个月，避免反复冻融。适用于科研场景中评估疾病模型（如哮喘、过敏或寄生虫感染）的嗜酸性粒细胞激活程度，支持多种样本类型的平行检测，为研究Th2型免疫反应、组织炎症机制及药物干预效果提供可靠工具。
缩写：

ECP
种属：

Mus musculus (Mouse)
样本类型：

serum, plasma, cell culture supernates, tissue homogenates
检测范围：

0.156 ng/mL-10 ng/mL
灵敏度：

0.039 ng/mL
反应时间：

1-5h
样本体积：

50-100ul
检测波长：

450 nm
研究领域：

Others
测定原理：

quantitative
测定方法：

Sandwich

精密度：

线性度：

To assess the linearity of the assay, samples were spiked with high concentrations of mouse ECP in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
	Sample	Serum(n=4)
1:1	Average %	91
	Range %	86-98
1:2	Average %	97
	Range %	92-106
1:4	Average %	93
	Range %	88-99
1:8	Average %	89
	Range %	83-95

回收率：

The recovery of mouse ECP spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.

Sample Type	Average % Recovery	Range
Serum (n=5)	92	87-97
EDTA plasma (n=4)	95	89-99

标准曲线：

These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.