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Mouse diamine oxidase,DAO ELISA Kit

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CUSABIO小鼠二胺氧化酶（DAO）酶联免疫检测试剂盒（货号：CSB-E10090m），采用双抗体夹心法定量检测血清、血浆及组织匀浆中的DAO含量，检测灵敏度达15.6 mU/mL，线性范围覆盖15.6-1000 mU/mL。DAO是一种存在于小肠上皮细胞和肾脏中的酶类，负责催化组胺、腐胺等生物活性胺的氧化脱氨反应，其活性变化与肠道屏障功能、炎症反应及代谢调控密切相关，是评估肠道黏膜完整性及代谢性疾病机制研究的生物标志物。该试剂盒适用于科研实验中DAO水平的动态监测，例如在肠道损伤模型、代谢综合征研究或药物干预实验中，通过检测不同生理/病理条件下血清、血浆及组织样本的DAO表达量，为探索肠道屏障功能调节机制、炎症相关疾病病理进程提供可靠的数据支持。试剂盒操作便捷，标准曲线稳定，可满足多种实验设计需求。
别名：

Aoc1 ELISA kit; Abp1Amiloride-sensitive amine oxidase [copper-containing] ELISA kit; DAO ELISA kit; Diamine oxidase ELISA kit; EC 1.4.3.22 ELISA kit; Amiloride-binding protein 1 ELISA kit; Amine oxidase copper domain-containing protein 1 ELISA kit; Histaminase ELISA kit
缩写：

ABP1
Uniprot No.：

Q8JZQ5
种属：

Mus musculus (Mouse)
样本类型：

serum, plasma, tissue homogenates
检测范围：

15.6 mU/mL-1000 mU/mL
灵敏度：

3.9 mU/mL
反应时间：

1-5h
样本体积：

50-100ul
检测波长：

450 nm
研究领域：

Signal Transduction
测定原理：

quantitative
测定方法：

Sandwich

精密度：

线性度：

To assess the linearity of the assay, samples were spiked with high concentrations of mouse DAO in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
	Sample	Serum(n=4)
1:1	Average %	84
	Range %	81-87
1:2	Average %	95
	Range %	91-99
1:4	Average %	91
	Range %	88-96
1:8	Average %	96
	Range %	93-99

回收率：

The recovery of mouse DAO spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.

Sample Type	Average % Recovery	Range
Serum (n=5)	109	104-112
EDTA plasma (n=4)	85	81-89

标准曲线：

These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

mU/ml	OD1	OD2	Average	Corrected
1000	2.879	2.752	2.816	2.607
500	2.301	2.216	2.259	2.050
250	1.584	1.560	1.572	1.363
125	0.945	0.932	0.939	0.730
62.5	0.655	0.672	0.664	0.455
31.2	0.532	0.542	0.537	0.328
15.6	0.412	0.407	0.410	0.201
0	0.211	0.206	0.209

本试剂盒所含材料：
- A micro ELISA plate --- The 96-well plate has been pre-coated with an anti-mouse DAO antibody. This dismountable microplate can be divided into 12 x 8 strip plates.
- Two vials lyophilized standard ---Dilute a bottle of the standard at dilution series, read the OD values, and then draw a standard curve.
- One vial Biotin-labeled DAO antibody (100 x concentrate) (120 μl/bottle) ---Act as the detection antibody.
- One vial HRP-avidin (100 x concentrate) (120 μl/bottle) ---Bind to the detection antibody and react with the TMB substrate to make the solution chromogenic.
- One vial Biotin-antibody Diluent (15 ml/bottle) ---Dilute the Biotin-antibody.
- One vial HRP-avidin Diluent (15 ml/bottle) ---Dilute the HRP-avidin solution.
- One vial Sample Diluent (50 ml/bottle)---Dilute the sample to an appropriate concentration.
- One vial Wash Buffer (25 x concentrate) (20 ml/bottle) ---Wash away unbound or free substances.
- One vial TMB Substrate (10 ml/bottle) ---Act as the chromogenic agent. TMB interacts with HRP, eliciting the solution turns blue.
- One vial Stop Solution (10 ml/bottle) ---Stop the color reaction. The solution color immediately turns from blue to yellow.
- Four Adhesive Strips (For 96 wells) --- Cover the microplate when incubation.
- An instruction manual
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本试剂盒不含材料：
- A microplate reader capable of measuring absorbance at 450 nm, with the correction wavelength set at 540 nm or 570 nm.
- An incubator can provide stable incubation conditions up to 37°C±5°C.
- Centrifuge
- Vortex
- Squirt bottle, manifold dispenser, or automated microplate washer
- Absorbent paper for blotting the microtiter plate
- 50-300ul multi-channel micropipette
- Pipette tips
- Single-channel micropipette with different ranges
- 100ml and 500ml graduated cylinders
- Deionized or distilled water
- Timer
- Test tubes for dilution
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数据处理：

ELISA操作视频及标曲绘制软件下载
货期：

3-5 working days

Intra-assay Precision (Precision within an assay): CV%<8%

Three samples of known concentration were tested twenty times on one plate to assess.

Inter-assay Precision (Precision between assays): CV%<10%

Three samples of known concentration were tested in twenty assays to assess.