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  4. Mouse coagulation factor Ⅺ,FⅪ ELISA Kit

Mouse coagulation factor Ⅺ,FⅪ ELISA Kit

说明书下载
  • 中文名称：
    小鼠凝血因子Ⅺ(FⅪ)酶联免疫试剂盒
  • 货号：
    CSB-E17818m
  • 规格：
    96T/48T
  • 价格：
    ￥3600/￥2500
  • 其他：
CUSABIO Wins 2024 Bioz Rapid Star™ Award in the ELISA category

产品详情

  • 产品描述：
    CUSABIO小鼠凝血因子Ⅺ(FⅪ)酶联免疫检测试剂盒（货号：CSB-E17818m），采用竞争性酶联免疫吸附法实现定量检测，适用于血清、血浆及组织匀浆样本中FⅪ蛋白含量的精准分析。FⅪ作为丝氨酸蛋白酶家族成员，通过激活内源性凝血途径促进凝血酶生成，在维持凝血-抗凝平衡中起关键作用，其异常表达与血栓性疾病等病理过程相关。本试剂盒检测灵敏度达0.47 ng/mL，线性范围覆盖0.47-30 ng/mL，可稳定检测微量样本中的目标蛋白。实验采用预包被特异性抗体的96孔板，通过样本中FⅪ与标记物的竞争性结合实现定量检测，操作流程包含标准品梯度稀释、样本孵育、显色反应等标准化步骤。该产品可应用于凝血机制研究、血栓性疾病动物模型分析、抗凝药物开发等科研领域，尤其适用于心血管疾病模型小鼠的血液样本检测，以及肝、肾等组织中FⅪ表达水平的比较研究，为探索凝血因子调控机制提供可靠工具。
  • 别名：
    F11Coagulation factor XI ELISA kit; FXI ELISA kit; EC 3.4.21.27 ELISA kit; Plasma thromboplastin antecedent ELISA kit; PTA) [Cleaved into: Coagulation factor XIa heavy chain; Coagulation factor XIa light chain] ELISA kit
  • 缩写：
    F
  • Uniprot No.：
  • 种属：
    Mus musculus (Mouse)
  • 样本类型：
    serum, plasma, tissue homogenates
  • 检测范围：
    0.47 ng/mL-30 ng/mL
  • 灵敏度：
    0.12 ng/mL
  • 反应时间：
    1-5h
  • 样本体积：
    50-100ul
  • 检测波长：
    450 nm
  • 研究领域：
    Blood Coagulation
  • 测定原理：
    quantitative
  • 测定方法：
    Competitive
  • 精密度：
    Intra-assay Precision (Precision within an assay): CV%<8%      
    Three samples of known concentration were tested twenty times on one plate to assess.  
    Inter-assay Precision (Precision between assays): CV%<10%      
    Three samples of known concentration were tested in twenty assays to assess.    
                 
  • 线性度：
    To assess the linearity of the assay, samples were spiked with high concentrations of mouse FⅪ in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
    　 Sample Serum(n=4)  
    1:100 Average % 95  
    Range % 90-101  
    1:200 Average % 87  
    Range % 84-90  
    1:400 Average % 102  
    Range % 98-106  
    1:800 Average % 93  
    Range % 89-97  
  • 回收率：
    The recovery of mouse FⅪ spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.
    Sample Type Average % Recovery Range  
    Serum (n=5) 94 90-98  
    EDTA plasma (n=4) 98 94-103  
                 
                 
  • 标准曲线：
    These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.
    ng/ml OD1 OD2 Average    
    30 0.147 0.139 0.143    
    15 0.219 0.225 0.222    
    7.5 0.345 0.326 0.336    
    3.75 0.513 0.536 0.525    
    1.875 0.857 0.862 0.860    
    0.94 1.179 1.198 1.189    
    0.47 1.538 1.559 1.549    
    0 2.533 2.542 2.538    
  • 数据处理：
    ELISA操作视频及标曲绘制软件下载
  • 货期：
    3-5 working days

产品评价

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F11 Proteins

靶点详情

  • 功能：
    Factor XI triggers the middle phase of the intrinsic pathway of blood coagulation by activating factor IX.
  • 基因功能参考文献：
    1. FXI(-/-) mice have a mild hemostatic defect after injury to the saphenous vein but not after transection of the tail or vessels in the abdominal wall. PMID: 28677246
    2. FXI protects against sepsis derived from Streptococcus pneumoniae or Klebsiella pneumoniae pneumonia at least in part by enhancing the phagocytic capacity of neutrophils by a mechanism that is independent of activation via FXIIa. PMID: 28492700
    3. FXI attenuates part of the allergic response to repeated administration of house dust mite in the airways by a mechanism that is independent of activation via FXII. PMID: 27913422
    4. Thrombin-independent contribution of tissue factor to inflammation and cardiac hypertrophy in a mouse model of sickle cell disease. PMID: 26817955
    5. FXI deprivation was shown to slow down atherogenesis in Apoe knockout mice. PMID: 26800563
    6. anion-binding sites on FXIa that are required for expression of heparin's cofactor activity during protease inhibition by antithrombin are also required for expression of polyP cofactor activity during FXI activation PMID: 24118982
    7. Factor XI antisense oligonucleotides safely prevent thrombus formation on acutely ruptured atherosclerotic plaques in mice. PMID: 24947525
    8. Studies indicate that targeting factor XI (FXI) by knocking out the gene prevents arterial and vein thrombosis. PMID: 23929304
    9. Protective roles for fibrin, tissue factor, plasminogen activator inhibitor-1, and thrombin activatable fibrinolysis inhibitor, but not factor XI, during defense against the gram-negative bacterium Yersinia enterocolitica. PMID: 21724997
    10. fXI and fXII contribute to thrombus formation even when factor VIIa/tissue factor initiates thrombosis. PMID: 20634381
    11. Gene targeting of tissue factor, factor X, and factor VII in mice: their involvement in embryonic development PMID: 11841337
    12. FXI is essential for thrombus formation following FeCl3-induced injury of the carotid artery in the mouse. PMID: 12008966
    13. FIX and FXI deficiency cause similar effects in the carotid artery injury model PMID: 15733058
    14. fXI may have functions in regulation of inflammation or tissue repair distinct from its role in coagulation PMID: 15985536
    15. apolipoprotein E receptor 2 (ApoER2, LRP8), a member of the LDL receptor family, is a platelet receptor for FXI. PMID: 19661487

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  • 亚细胞定位：
    Secreted.
  • 蛋白家族：
    Peptidase S1 family, Plasma kallikrein subfamily
  • 数据库链接：

    KEGG: mmu:109821

    STRING: 10090.ENSMUSP00000034064

    UniGene: Mm.33326

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九游平台-九游(中国) | 米兰体育-米兰体育（中国） | 开云手机在线登陆入口 | 江南官方体育网页版 | 开云网页版 | 开云官方在线入口 | 开云电子体育 | 开云官方链接 | 江南网页版页面登录 |