Your Good Partner in Biology Research

中文简体

CSF2｜ CTLA4｜ DLL3｜ EGFR｜ IL4｜ IL4R｜ LAG3｜ MSTN｜ NECTIN4｜ NT5E｜ SEZ6｜ TM4SF1｜ TNFSF13｜ TPO

  1. 首页
  2. 试剂盒
  3. 酶联免疫试剂盒
  4. Mouse coagulation factor Ⅶ,FⅦ ELISA Kit

Mouse coagulation factor Ⅶ,FⅦ ELISA Kit

说明书下载
  • 中文名称：
    小鼠凝血因子Ⅶ(FⅦ)酶联免疫试剂盒
  • 货号：
    CSB-E17817m
  • 规格：
    96T/48T
  • 价格：
    ￥3600/￥2500
  • 其他：
CUSABIO Wins 2024 Bioz Rapid Star™ Award in the ELISA category

产品详情

  • 产品描述：
    CUSABIO小鼠凝血因子Ⅶ（FⅦ）酶联免疫检测试剂盒（货号CSB-E17817m），采用双抗体夹心法原理实现定量检测，适用于血清、血浆及组织匀浆样本中FⅦ蛋白的精准分析。凝血因子Ⅶ是维生素K依赖性丝氨酸蛋白酶前体，在血液凝固级联反应中通过与组织因子结合激活FⅨ和FⅩ，对止血和血栓形成具有关键调控作用。本试剂盒检测灵敏度为0.45 ng/mL，线性检测范围覆盖0.45-30 ng/mL，实验操作仅需常规样本前处理，可在4小时内完成检测流程。该产品适用于凝血机制基础研究、抗凝药物开发评估以及血栓性疾病或出血性疾病小鼠模型中FⅦ蛋白水平的动态监测，为研究凝血调控网络提供可靠工具。
  • 别名：
    F7 ELISA Kit; Cf7 ELISA Kit; Coagulation factor VII ELISA Kit; EC 3.4.21.21 ELISA Kit; Serum prothrombin conversion accelerator) [Cleaved into: Factor VII light chain; Factor VII heavy chain] ELISA Kit
  • 缩写：
    F7
  • Uniprot No.：
  • 种属：
    Mus musculus (Mouse)
  • 样本类型：
    serum, plasma, tissue homogenates
  • 检测范围：
    0.45 ng/mL-30 ng/mL
  • 灵敏度：
    0.11 ng/mL
  • 反应时间：
    1-5h
  • 样本体积：
    50-100ul
  • 检测波长：
    450 nm
  • 研究领域：
    Blood Coagulation
  • 测定原理：
    quantitative
  • 测定方法：
    Sandwich
  • 精密度：
    Intra-assay Precision (Precision within an assay): CV%<8%        
    Three samples of known concentration were tested twenty times on one plate to assess.    
    Inter-assay Precision (Precision between assays): CV%<10%        
    Three samples of known concentration were tested in twenty assays to assess.      
                   
  • 线性度：
    To assess the linearity of the assay, samples were spiked with high concentrations of mouse FⅦ in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.  
    　 Sample Serum(n=4)    
    1:1000 Average % 92    
    Range % 86-96    
    1:2000 Average % 97    
    Range % 92-102    
    1:4000 Average % 93    
    Range % 88-97    
    1:8000 Average % 89    
    Range % 84-94    
  • 回收率：
    The recovery of mouse FⅦ spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.  
     
    Sample Type Average % Recovery Range    
    Serum (n=5) 92 88-95    
    EDTA plasma (n=4) 108 103-114    
                   
                   
  • 标准曲线：
    These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.  
     
    ng/ml OD1 OD2 Average Corrected    
    30 2.468 2.445 2.457 2.324    
    15 2.115 1.951 2.033 1.900    
    7.5 1.503 1.440 1.472 1.339    
    3.75 0.845 0.790 0.818 0.685    
    1.8 0.626 0.593 0.610 0.477    
    0.9 0.381 0.362 0.372 0.239    
    0.45 0.274 0.252 0.263 0.130    
    0 0.137 0.129 0.133 　    
  • 数据处理：
    ELISA操作视频及标曲绘制软件下载
  • 货期：
    3-5 working days

产品评价

相关产品

F7 Proteins

靶点详情

  • 功能：
    Initiates the extrinsic pathway of blood coagulation. Serine protease that circulates in the blood in a zymogen form. Factor VII is converted to factor VIIa by factor Xa, factor XIIa, factor IXa, or thrombin by minor proteolysis. In the presence of tissue factor and calcium ions, factor VIIa then converts factor X to factor Xa by limited proteolysis. Factor VIIa will also convert factor IX to factor IXa in the presence of tissue factor and calcium.
  • 基因功能参考文献：
    1. Leu8 is crucial for FVIIa-EPCR binding; this study characterizes its interaction in vivo in mice PMID: 28035745
    2. FVIIa-antithrombin but not FVIIa is a ligand for LRP1, and LRP1 contributes to the clearance of FVIIa-antithrombin in vivo PMID: 27614059
    3. FVIIa binding to EPCR leads to a barrier protective effect in vivo PMID: 24977291
    4. Murine FVIIa binds poorly to murine EPCR. PMID: 22370814
    5. GlycoPEGylated rFVIIa (N7-GP) has a prolonged hemostatic effect in hemophilic mice compared with rFVIIa PMID: 21366858
    6. Conclude that the fVII-targeted verteporfin photodynamic therapy that we report here is a novel and effective therapeutic with improved selectivity for the treatment of breast cancer. PMID: 20504328
    7. Recombinant FVIIa readily associates with the vascular endothelium and subsequently enters into extravascular spaces where it is likely to bind to tissue factor and is retained for extended time periods. PMID: 19943873
    8. true circadian rhythms for FVII were found PMID: 15604416
    9. Data suggest that long-term expression of murine activated factor VII is safe, but elevated levels cause premature mortality. PMID: 18398505
    10. FVIIa functions in the asthmatic response to an allergen by stimulating lung eosinophilia, airway hyperresponsiveness, and mucin production. PMID: 19286924
    11. Plasma elimination kinetics for factor VII are independent of its activation to factor VIIa and complex formation with plasma inhibitors. PMID: 19404533

    显示更多

    收起更多

  • 亚细胞定位：
    Secreted.
  • 蛋白家族：
    Peptidase S1 family
  • 组织特异性：
    Plasma and liver.
  • 数据库链接：

    KEGG: mmu:14068

    STRING: 10090.ENSMUSP00000033820

    UniGene: Mm.4827

Most popular with customers

产品中心
特别关注


X
九游平台-九游(中国) | 米兰体育-米兰体育（中国） | 开云手机在线登陆入口 | 江南官方体育网页版 | 开云网页版 | 开云官方在线入口 | 开云电子体育 | 开云官方链接 | 江南网页版页面登录 |
九游平台-九游(中国) | 米兰体育-米兰体育（中国） | 开云手机在线登陆入口 | 江南官方体育网页版 | 开云网页版 | 开云官方在线入口 | 开云电子体育 | 开云官方链接 | 江南网页版页面登录 |