Mouse circulating immune complex(CIC)ELISA Kit

产品详情

Intra-assay Precision (Precision within an assay): CV%<8%

Three samples of known concentration were tested twenty times on one plate to assess.

Inter-assay Precision (Precision between assays): CV%<10%

Three samples of known concentration were tested in twenty assays to assess.

产品描述：

小鼠循环免疫复合物(CIC)酶联免疫试剂盒（CSB-E16553m）为双抗夹心法ELISA试剂盒，定量检测血清、血浆样本中的CIC含量。小鼠循环免疫复合物（CIC）是体内抗原与抗体结合形成的复合物，多数情况下对机体有益，但在某些情况下，如免疫复合物沉积，可能引起组织损伤和疾病。CIC的研究涉及其形成机制、临床意义以及与疾病如类风湿关节炎的关系。试剂盒检测范围为15.6 ng/mL-1000 ng/mL，适用于小鼠自身免疫疾病模型构建、感染性疾病研究中免疫复合物沉积机制探索，或药物干预后免疫调节效应的评估，也可用于免疫学、病理学等基础研究中对样本中 CIC 水平进行检测以探究免疫调节机制或评估疾病模型状态。本品仅用于科研，不用于临床诊断，产品具体参数及操作步骤详见产品说明书。
别名：

N/A
缩写：

CIC
种属：

Mus musculus (Mouse)
样本类型：

serum, plasma
检测范围：

15.6 ng/mL-1000 ng/mL
灵敏度：

3.9 ng/mL
反应时间：

1-5h
样本体积：

50-100ul
检测波长：

450 nm
研究领域：

Others
测定原理：

quantitative
测定方法：

Sandwich

精密度：

线性度：

To assess the linearity of the assay, samples were spiked with high concentrations of mouse CIC in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
	Sample	Serum(n=4)
1:20	Average %	95
	Range %	89-103
1:40	Average %	90
	Range %	81-100
1:80	Average %	91
	Range %	86-99
1:160	Average %	95
	Range %	91-104

回收率：

The recovery of mouse CIC spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.

Sample Type	Average % Recovery	Range
Serum (n=5)	95	89-102
EDTA plasma (n=4)	96	90-104

标准曲线：

These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.