Mouse choline acetyltransterase(ChAT)ELISA Kit

中文名称：

小鼠胆碱乙酰转移酶（ChAT）酶联免疫试剂盒
货号：

CSB-E17524m
规格：

96T/48T
价格：

￥3600/￥2500
其他：

CUSABIO Wins 2024 Bioz Rapid Star™ Award in the ELISA category

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产品描述：

CUSABIO小鼠胆碱乙酰转移酶（ChAT）酶联免疫检测试剂盒，采用双抗体夹心法原理进行定量检测，适用于科研领域中对血清、血浆、组织匀浆及细胞培养上清液样本中ChAT蛋白含量的精准分析。胆碱乙酰转移酶是催化乙酰胆碱合成的关键酶，其表达水平与胆碱能神经元功能及神经递质调控密切相关，在神经退行性疾病模型研究、神经发育机制探索及药物干预效果评估中具有重要科研价值。试剂盒检测灵敏度高，线性范围为15.6-1000 pg/mL，可覆盖常规实验样本的浓度梯度需求。实验操作流程标准化，提供预包被特异性抗体的96孔板及配套试剂，仅需约4.5小时即可完成样本孵育、洗涤、显色及终止等步骤，通过酶标仪在450nm波长下读取吸光度值进行定量计算。该试剂盒严格验证了批内和批间重复性，确保实验数据的可靠性，为神经科学、药理学及基础医学研究提供稳定高效的检测工具。
别名：

ChatCholine O-acetyltransferase ELISA kit; CHOACTase ELISA kit; ChAT ELISA kit; Choline acetylase ELISA kit; EC 2.3.1.6 ELISA kit
缩写：

CHAT
Uniprot No.：

Q03059
种属：

Mus musculus (Mouse)
样本类型：

serum, plasma, tissue homogenates, cell culture supernates
检测范围：

15.6 pg/mL-1000 pg/mL
灵敏度：

3.9 pg/mL
反应时间：

1-5h
样本体积：

50-100ul
检测波长：

450 nm
研究领域：

Neuroscience
测定原理：

quantitative
测定方法：

Sandwich

精密度：

Intra-assay Precision (Precision within an assay): CV%<8%
Three samples of known concentration were tested twenty times on one plate to assess.
Inter-assay Precision (Precision between assays): CV%<10%
Three samples of known concentration were tested in twenty assays to assess.

线性度：

To assess the linearity of the assay, samples were spiked with high concentrations of mouse ChAT in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
	Sample	Serum(n=4)
1:100	Average %	92
	Range %	88-96
1:200	Average %	87
	Range %	82-91
1:400	Average %	103
	Range %	99-108
1:800	Average %	95
	Range %	90-100

回收率：

The recovery of mouse ChAT spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.

Sample Type	Average % Recovery	Range
Serum (n=5)	96	91-100
EDTA plasma (n=4)	92	88-96

标准曲线：

These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

pg/ml	OD1	OD2	Average	Corrected
1000	2.042	2.136	2.089	1.933
500	1.407	1.481	1.444	1.288
250	0.893	0.947	0.920	0.764
125	0.610	0.658	0.634	0.478
62.5	0.475	0.463	0.469	0.313
31.2	0.321	0.305	0.313	0.157
15.6	0.254	0.249	0.252	0.096
0	0.159	0.152	0.156

数据处理：

ELISA操作视频及标曲绘制软件下载
货期：

3-5 working days

引用文献

Neuroprotective effects of polygalacic acid on scopolamine-induced memory deficits in mice Changrun Guo. et al,Phytomedicine,2016

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功能：

Catalyzes the reversible synthesis of acetylcholine (ACh) from acetyl CoA and choline at cholinergic synapses.
蛋白家族：

Carnitine/choline acetyltransferase family
数据库链接：

STRING: 10090.ENSMUSP00000070865

UniGene: Mm.442817

Mouse choline acetyltransterase(ChAT)ELISA Kit

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