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中文名称:小鼠癌胚抗原(CEA)酶联免疫试剂盒
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货号:CSB-E13925m
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规格:96T/48T
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价格:¥3800/¥2500
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其他:
产品详情
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产品描述:CUSABIO小鼠癌胚抗原(CEA)酶联免疫检测试剂盒(货号:CSB-E13925m),采用双抗体夹心法定量检测小鼠血清、血浆及组织匀浆样本中的CEA含量,检测范围为62.5 pg/mL至4000 pg/mL。CEA是一种广谱性肿瘤相关糖蛋白,在多种上皮性肿瘤中异常高表达,常作为肿瘤发生、发展和转移的标志物,其在小鼠模型中的动态监测对癌症机制研究、治疗药物筛选及疗效评估具有重要意义。本试剂盒基于高特异性配对抗体开发,可精准捕获目标蛋白并排除样本基质干扰,灵敏度达62.5 pg/mL,适用于肿瘤模型构建、基因编辑动物验证、肿瘤微环境分析等科研场景。该试剂盒操作流程标准化,支持批量样本检测,兼容血清、血浆及组织匀浆等多种生物样本类型,为研究者提供稳定可靠的数据支持,助力肿瘤生物学、免疫治疗及转化医学等领域的实验探索。
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别名:Ceacam5 ELISA Kit; Psg30Carcinoembryonic antigen-related cell adhesion molecule 5 ELISA Kit; Pregnancy-specific glycoprotein 30 ELISA Kit
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缩写:
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Uniprot No.:
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种属:Mus musculus (Mouse)
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样本类型:serum, plasma, tissue homogenates
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检测范围:62.5 pg/mL-4000 pg/mL
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灵敏度:15.6 pg/mL
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反应时间:1-5h
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样本体积:50-100ul
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检测波长:450 nm
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研究领域:Cancer
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测定原理:quantitative
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测定方法:Sandwich
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精密度:
Intra-assay Precision (Precision within an assay): CV%<8% Three samples of known concentration were tested twenty times on one plate to assess. Inter-assay Precision (Precision between assays): CV%<10% Three samples of known concentration were tested in twenty assays to assess. -
线性度:
To assess the linearity of the assay, samples were spiked with high concentrations of mouse CEA in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay. Sample Serum(n=4) 1:1 Average % 90 Range % 85-93 1:2 Average % 98 Range % 95-100 1:4 Average % 102 Range % 97-107 1:8 Average % 94 Range % 91-96 -
回收率:
The recovery of mouse CEA spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section. Sample Type Average % Recovery Range Serum (n=5) 97 91-105 EDTA plasma (n=4) 93 90-97 -
标准曲线:
These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed. pg/ml OD1 OD2 Average Corrected 4000 2.522 2.635 2.579 2.402 2000 1.656 1.764 1.710 1.533 1000 1.047 1.095 1.071 0.894 500 0.637 0.604 0.621 0.444 250 0.366 0.390 0.378 0.201 125 0.275 0.281 0.278 0.101 62.5 0.212 0.230 0.221 0.044 0 0.171 0.183 0.177 -
数据处理:
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货期:3-5 working days
引用文献
相关产品
靶点详情
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功能:Cell surface glycoprotein that plays a role in cell adhesion, intracellular signaling and tumor progression. Mediates homophilic and heterophilic cell adhesion with other carcinoembryonic antigen-related cell adhesion molecules, such as CEACAM6. Plays a role as an oncogene by promoting tumor progression; induces resistance to anoikis of colorectal carcinoma cells.
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基因功能参考文献:
- a new mechanistic insight into how the CEA IgV-like N domain participates in cellular events that can have a macroscopic impact in terms of cancer progression and metastasis. PMID: 24388361
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亚细胞定位:Cell membrane; Lipid-anchor, GPI-anchor. Apical cell membrane. Cell surface.
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蛋白家族:Immunoglobulin superfamily, CEA family
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数据库链接:
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