Mouse anti-thyroid-globulin antibody,TGAB ELISA Kit

产品详情

产品描述：

CUSABIO小鼠抗甲状腺球蛋白抗体（ATGA/TGAB）定量检测试剂盒，采用竞争性酶联免疫吸附原理，适用于血清、血浆样本的科研分析。甲状腺球蛋白抗体是自身免疫性甲状腺疾病（如桥本甲状腺炎、Graves病）的重要标志物，在动物模型中常被用于研究甲状腺自身免疫反应的病理机制及药物干预效果评估。试剂盒检测范围覆盖1.25至20 U/mL，通过预包被的靶标抗原与样本中ATGA特异性结合，结合辣根过氧化物酶标记的检测系统实现定量分析，灵敏度达0.1 U/mL，批内与批间精密度分别控制在10%和15%以内。该试剂盒适用于实验室环境下的小鼠模型研究，包括甲状腺自身免疫疾病发病机理探索、免疫调节药物作用效果验证以及相关生物标志物动态监测等科研场景，实验过程需在37℃环境进行，总操作时长约3.5小时，提供标准品、预混试剂及完整操作说明书，需自备酶标仪等基础实验设备。
缩写：

TG Ab
种属：

Mus musculus (Mouse)
样本类型：

serum, plasma
检测范围：

1.25 U/mL-20 U/mL
灵敏度：

1.25 U/mL
反应时间：

1-5h
样本体积：

50-100ul
检测波长：

450 nm
研究领域：

Others
测定原理：

quantitative
测定方法：

Competitive

精密度：

Intra-assay Precision (Precision within an assay): CV%<15%
Three samples of known concentration were tested twenty times on one plate to assess.
Inter-assay Precision (Precision between assays):CV%<15%
Three samples of known concentration were tested in twenty assays to assess.

线性度：

To assess the linearity of the assay, samples were spiked with high concentrations of mouse TGAB in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
	Sample	Serum(n=4)
1:1	Average %	90
	Range %	85-101
1:2	Average %	89
	Range %	85-98
1:4	Average %	92
	Range %	84-100
1:8	Average %	95
	Range %	90-100

回收率：

The recovery of mouse TGAB spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.

Sample Type	Average % Recovery	Range
Serum (n=5)	95	90-103
EDTA plasma (n=4)	95	90-105

标准曲线：

These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

平均分:

5.0分 - 2 个评价

样品类型：血清

样品信息：小鼠

稀释比：没有稀释

产品评价：我用CSB-E09543m检测正常小鼠血清，其OD为0.5003，模型组OD为0.1912

By 王老师

样品类型：血清

样品信息：大鼠

稀释比：其他 1：10

产品评价：我用CSB-E09543m检测自身免疫性甲状腺炎大鼠，稀释比例为1：10，用小鼠盒子检测大鼠，实验结果也较为准确，符合趋势。期望能出大鼠TGAB的盒子，我们实验室需求量很大。

By 丁老师