Mouse anti-double stranded DNA antibody(IgM)ELISA Kit

Mouse anti-double stranded DNA antibody(IgM)ELISA Kit

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中文名称：

小鼠抗双链DNA抗体/天然DNA抗体(IgM)酶联免疫试剂盒
货号：

CSB-E12911m
规格：

96T/48T
价格：

￥3200/￥2500
其他：

CUSABIO Wins 2024 Bioz Rapid Star™ Award in the ELISA category

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产品描述：

小鼠抗双链DNA抗体/天然DNA抗体(IgM)酶联免疫试剂盒（CSB-E12911m）为捕获法ELISA试剂盒，定量检测血清、血浆样本中的dsDNA Ab (IgM)含量。dsDNA Ab (IgM) 即抗双链DNA抗体（IgM类）。在系统性红斑狼疮等自身免疫病中，机体免疫系统失调产生该抗体，它可与体内双链DNA结合形成免疫复合物，沉积在组织器官引发炎症损伤。深入研究其作用机制，对疾病诊断和治疗有重要意义。试剂盒检测范围为1.56 ng/mL-100 ng/mL，该产品可广泛应用于自身免疫疾病机制研究、药物开发中抗体水平动态监测、免疫调控实验评估等科研场景，为动物模型中的免疫应答分析及病理机制探索提供可靠工具。本品仅用于科研，不用于临床诊断，产品具体参数及操作步骤详见产品说明书。
缩写：

dsDNA Ab (IgM)
种属：

Mus musculus (Mouse)
样本类型：

serum, plasma
检测范围：

1.56 ng/mL-100 ng/mL
灵敏度：

0.39 ng/mL
反应时间：

1-5h
样本体积：

50-100ul
检测波长：

450 nm
研究领域：

Others
测定原理：

quantitative
测定方法：

Capture ELISA

精密度：

线性度：

To assess the linearity of the assay, samples were spiked with high concentrations of mouse anti-double stranded DNA antibody (IgM) (dsDNA Ab (IgM)) in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
	Sample	Serum(n=4)
1:1	Average %	95
	Range %	85-105
1:2	Average %	89
	Range %	85-98
1:4	Average %	93
	Range %	85-97
1:8	Average %	95
	Range %	89-110

回收率：

The recovery of mouse anti-double stranded DNA antibody (IgM) (dsDNA Ab (IgM)) spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.

Sample Type	Average % Recovery	Range
Serum (n=5)	94	83-101
EDTA plasma (n=4)	97	87-106

标准曲线：

These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

ng/ml	OD1	OD2	Average	Corrected
100	2.501	2.531	2.516	2.377
50	2.178	2.203	2.191	2.052
25	1.803	1.822	1.813	1.674
12.5	1.254	1.268	1.261	1.122
6.25	0.731	0.745	0.738	0.599
3.12	0.405	0.397	0.401	0.262
1.56	0.255	0.249	0.252	0.113
0	0.138	0.140	0.139

数据处理：

ELISA操作视频及标曲绘制软件下载
货期：

3-5 working days

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IL-38, a potential therapeutic agent for lupus, inhibits lupus progression WD Xu,Inflammation research,2023
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Jieduquyuziyin prescription promotes the efficacy of prednisone via upregulating Nrf2 in MRL/lpr kidneys L Du,Journal of ethnopharmacology,2022
Inhibition of GSK‐3β/HIF‐1α pathway alleviates NLRP3‐mediated pyroptosis induced by high glucose in renal tubular epithelial cells J Wan,Experimental physiology,2022
A Tissue-Tended Mycophenolate-Modified Nanoparticle Alleviates Systemic Lupus Erythematosus in MRL/Lpr Mouse Model Mainly by Promoting Local M2-Like Macrophagocytes Polarization B Jiang,International journal of nanomedicine,2022
A Tissue-Tended Mycophenolate-Modified Nanoparticle Alleviates Systemi BT Tok,/,2022
Vasoactive intestinal peptide ameliorates renal injury in a pristane-induced lupus mouse model by modulating Th17/Treg balance Dongdong Fu, et al,Bmc Nephrology,2019
Apoptotic cell-mimicking gold nanocages loaded with LXR agonist for attenuating the progression of murine systemic lupus erythematosus Xu N, et al,Biomaterials,2019
RNAi Silencing of HIF-1α Ameliorates Lupus Development in MRL/lpr Mice Wei Zhao.et al,Inflammation,2018

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Intra-assay Precision (Precision within an assay): CV%<8%

Three samples of known concentration were tested twenty times on one plate to assess.

Inter-assay Precision (Precision between assays): CV%<10%

Three samples of known concentration were tested in twenty assays to assess.