Mouse anti-Smith antibody ELISA Kit

产品详情

Intra-assay Precision (Precision within an assay): CV%<8%

Three samples of known concentration were tested twenty times on one plate to assess.

Inter-assay Precision (Precision between assays): CV%<10%

Three samples of known concentration were tested in twenty assays to assess.

产品描述：

CUSABIO定量检测小鼠血清或血浆中抗Smith抗体（Sm）的酶联免疫试剂盒（货号CSB-E15976m），采用双抗体夹心法原理，检测灵敏度范围为0.625-40 ng/mL，适用于科研领域中对系统性红斑狼疮（SLE）相关模型的抗体水平分析。Sm抗体是SLE的特异性生物标志物之一，其与核小体中的Sm蛋白复合物结合可引发自身免疫反应，在疾病机制研究中具有重要价值。本试剂盒通过高特异性抗体对实现精准检测，样本类型涵盖血清和血浆，操作流程标准化，可广泛应用于小鼠疾病模型中自身抗体动态监测、药物疗效评估或免疫病理学研究，为探索SLE发病机理及潜在治疗靶点提供可靠工具。实验全程无需复杂预处理，标准曲线覆盖低浓度至生理病理范围内的抗体含量，满足科研实验的定量分析需求。
缩写：

Sm Ab
种属：

Mus musculus (Mouse)
样本类型：

serum, plasma
检测范围：

0.625 ng/mL-40 ng/mL
灵敏度：

0.156 ng/mL
反应时间：

1-5h
样本体积：

50-100ul
检测波长：

450 nm
研究领域：

Immunology
测定原理：

quantitative
测定方法：

Sandwich

精密度：

线性度：

To assess the linearity of the assay, samples were spiked with high concentrations of mouse anti-smith antibody in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
	Sample	Serum(n=4)
1:1	Average %	88
	Range %	81-95
1:2	Average %	95
	Range %	90-100
1:4	Average %	93
	Range %	92-101
1:8	Average %	93
	Range %	82-98

回收率：

The recovery of mouse anti-smith antibody spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.

Sample Type	Average % Recovery	Range
Serum (n=5)	92	85-99
EDTA plasma (n=4)	95	90-100

标准曲线：

These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.