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  4. Mouse angiotensinogen (aGT) ELISA Kit

Mouse angiotensinogen (aGT) ELISA Kit

文献引用 (6) 说明书下载
  • 中文名称：
    小鼠血管紧张素原(aGT)酶联免疫试剂盒
  • 货号：
    CSB-E08566m
  • 规格：
    96T/48T
  • 价格：
    ￥3200/￥2500
  • 其他：
CUSABIO Wins 2024 Bioz Rapid Star™ Award in the ELISA category

产品详情

  • 产品描述：
    CUSABIO小鼠血管紧张素原（aGT）酶联免疫检测试剂盒（货号：CSB-E08566m），基于夹心法原理实现定量检测，适用于血清、血浆、细胞培养上清及组织匀浆等多种样本类型。血管紧张素原是肾素-血管紧张素系统（RAS）的关键前体蛋白，通过转化为血管紧张素II参与血压调节、体液平衡及心血管功能调控，在高血压、肾脏疾病等病理机制研究中具有重要价值。该试剂盒检测范围为3.12-200 ng/mL，可精准覆盖生理及实验条件下aGT的浓度变化，操作流程包含标准品梯度稀释、样本孵育、生物素标记抗体结合及显色反应等步骤，配套提供预包被捕获抗体的酶标板及完整实验试剂。其高特异性和灵敏度使其适用于评估疾病模型小鼠的RAS系统激活状态、药物干预后aGT表达水平变化，或体外细胞实验中血管紧张素原分泌动态研究，为心血管、内分泌及肾脏相关基础科研提供可靠工具。
  • 别名：
    Agt ELISA kit; Serpina8Angiotensinogen ELISA kit; Serpin A8) [Cleaved into: Angiotensin-1 ELISA kit; Angiotensin 1-10 ELISA kit; Angiotensin I ELISA kit; Ang I); Angiotensin-2 ELISA kit; Angiotensin 1-8 ELISA kit; Angiotensin II ELISA kit; Ang II); Angiotensin-3 ELISA kit; Angiotensin 2-8 ELISA kit; Angiotensin III ELISA kit; Ang III ELISA kit; Des-Asp[1]-angiotensin II); Angiotensin-4 ELISA kit; Angiotensin 3-8 ELISA kit; Angiotensin IV ELISA kit; Ang IV); Angiotensin 1-9; Angiotensin 1-7; Angiotensin 1-5; Angiotensin 1-4] ELISA kit
  • 缩写：
    AGT
  • Uniprot No.：
  • 种属：
    Mus musculus (Mouse)
  • 样本类型：
    serum, plasma, cell culture supernates, tissue homogenates
  • 检测范围：
    3.12 ng/mL-200 ng/mL
  • 灵敏度：
    0.78 ng/mL
  • 反应时间：
    1-5h
  • 样本体积：
    50-100ul
  • 检测波长：
    450 nm
  • 研究领域：
    Cardiovascular
  • 测定原理：
    quantitative
  • 测定方法：
    Sandwich
  • 精密度：
    Intra-assay Precision (Precision within an assay): CV%<8%
    Three samples of known concentration were tested twenty times on one plate to assess.
    Inter-assay Precision (Precision between assays): CV%<10%
    Three samples of known concentration were tested in twenty assays to assess.
  • 线性度：
    To assess the linearity of the assay, samples were spiked with high concentrations of mouse aGT in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
    　 Sample Serum(n=4)
    1:100 Average % 97
    Range % 92-101
    1:200 Average % 102
    Range % 98-110
    1:400 Average % 91
    Range % 86-95
    1:800 Average % 93
    Range % 89-97
  • 回收率：
    The recovery of mouse aGT spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.
    Sample Type Average % Recovery Range
    Serum (n=5) 89 85-93
    EDTA plasma (n=4) 84 80-88
  • 标准曲线：
    These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.
    ng/ml OD1 OD2 Average Corrected
    200 2.584 2.445 2.515 2.410
    100 1.692 1.573 1.633 1.528
    50 0.951 1.016 0.984 0.879
    25 0.647 0.662 0.655 0.550
    12.5 0.359 0.339 0.349 0.244
    6.25 0.236 0.221 0.229 0.124
    3.12 0.183 0.187 0.185 0.080
    0 0.102 0.108 0.105 　
  • 数据处理：
    ELISA操作视频及标曲绘制软件下载
  • 货期：
    3-5 working days

引用文献

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Agt Proteins

靶点详情

  • 功能：
    Essential component of the renin-angiotensin system (RAS), a potent regulator of blood pressure, body fluid and electrolyte homeostasis.; acts directly on vascular smooth muscle as a potent vasoconstrictor, affects cardiac contractility and heart rate through its action on the sympathetic nervous system, and alters renal sodium and water absorption through its ability to stimulate the zona glomerulosa cells of the adrenal cortex to synthesize and secrete aldosterone.; stimulates aldosterone release.; is a ligand for the G-protein coupled receptor MAS1. Has vasodilator and antidiuretic effects. Has an antithrombotic effect that involves MAS1-mediated release of nitric oxide from platelets.
  • 亚细胞定位：
    Secreted.
  • 蛋白家族：
    Serpin family
  • 组织特异性：
    Expressed by the liver and secreted in plasma.
  • 数据库链接：

    STRING: 10090.ENSMUSP00000066488

    UniGene: Mm.301626

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九游平台-九游(中国) | 米兰体育-米兰体育（中国） | 开云手机在线登陆入口 | 江南官方体育网页版 | 开云网页版 | 开云官方在线入口 | 开云电子体育 | 开云官方链接 | 江南网页版页面登录 |