Mouse acetyl-CoA ELISA Kit

产品详情

Intra-assay Precision (Precision within an assay): CV%<8%

Three samples of known concentration were tested twenty times on one plate to assess.

Inter-assay Precision (Precision between assays): CV%<10%

Three samples of known concentration were tested in twenty assays to assess.

产品描述：

小鼠乙酰辅酶A酶联免疫试剂盒（CSB-E12936m）为双抗夹心法ELISA试剂盒，定量检测血清、血浆、组织培养上清液、组织匀浆样本中的acetyl-CoA含量。Acetyl - CoA即乙酰辅酶A，是生物体代谢关键物质，在糖、脂肪、蛋白质代谢中起枢纽作用。研究发现，它参与三羧酸循环、脂肪酸合成等多种代谢途径，其水平变化会影响细胞代谢平衡，也与糖尿病、癌症等疾病的发生发展可能相关。试剂盒检测范围为0.78 ng/mL-50 ng/mL，适用于研究能量代谢、脂肪酸合成及细胞调控等机制，特别针对小鼠模型中的乙酰辅酶A代谢通路探索，例如在代谢性疾病、营养干预或药物作用等科研场景中，可通过多种生物样本进行定量分析，为体外实验和离体组织研究提供可靠工具。满足科研工作者对乙酰辅酶A相关分子机制的高通量研究需求。本品仅用于科研，不用于临床诊断，产品具体参数及操作步骤详见产品说明书。
缩写：

acetyl-CoA
种属：

Mus musculus (Mouse)
样本类型：

serum, plasma, cell culture supernates, tissue homogenates
检测范围：

0.78 ng/mL-50 ng/mL
灵敏度：

0.195 ng/mL
反应时间：

1-5h
样本体积：

50-100ul
检测波长：

450 nm
研究领域：

Signal Transduction
测定原理：

quantitative
测定方法：

Sandwich

精密度：

线性度：

To assess the linearity of the assay, samples were spiked with high concentrations of mouse Acetyl-CoA in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
	Sample	Serum(n=4)
1:20	Average %	91
	Range %	87-93
1:40	Average %	103
	Range %	100-105
1:80	Average %	93
	Range %	87-96
1:160	Average %	98
	Range %	93-101

回收率：

The recovery of mouse Acetyl-CoA spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.

Sample Type	Average % Recovery	Range
Serum (n=5)	94	89-97
EDTA plasma (n=4)	96	92-98

标准曲线：

These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.