Mouse Vitamin K-dependent protein S(PROS1) ELISA kit

Mouse Vitamin K-dependent protein S(PROS1) ELISA kit

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中文名称：

小鼠维生素K依赖性蛋白S(PROS1)酶联免疫试剂盒
货号：

CSB-EL018754MO
规格：

96T/48T
价格：

￥3600/￥2500
其他：

CUSABIO Wins 2024 Bioz Rapid Star™ Award in the ELISA category

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CUSABIO小鼠维生素K依赖性蛋白S（PROS1）酶联免疫吸附测定试剂盒（货号：CSB-EL018754MO），采用竞争法定量检测血清、血浆及组织匀浆样本中的PROS1蛋白含量，检测范围为6.25-400 ng/mL，灵敏度达6.25 ng/mL。PROS1是一种由肝脏合成的多功能糖蛋白，在抗凝血系统中通过与活化蛋白C结合抑制凝血因子Va和VIIIa，同时参与调节补体激活通路，对维持凝血平衡和免疫稳态具有重要作用。该试剂盒包含预包被96孔板、系列浓度标准品、生物素标记检测抗体及HRP标记链霉亲和素等核心组分，适用于科研场景下的小鼠模型研究，例如探究凝血功能障碍机制、血栓性疾病动物模型的蛋白表达动态变化，或评估肝肾功能异常对PROS1合成的影响。其宽泛的线性范围与多类型样本兼容性（包括组织匀浆）可为心血管疾病、免疫调节及器官特异性研究提供可靠的数据支持，操作流程可在4.5小时内完成。
别名：

Pros1 ELISA Kit; ProsVitamin K-dependent protein S ELISA Kit
缩写：

PROS1
Uniprot No.：

Q08761
种属：

Mus musculus (Mouse)
样本类型：

serum, plasma, tissue homogenates
检测范围：

6.25 ng/mL-400 ng/mL
灵敏度：

1.56 ng/mL
反应时间：

1-5h
样本体积：

50-100ul
检测波长：

450 nm
研究领域：

Cardiovascular
测定原理：

quantitative
测定方法：

Competitive

精密度：

线性度：

To assess the linearity of the assay, samples were spiked with high concentrations of human APOC4 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
	Sample	Serum(n=4)
1:200	Average %	92
Range %	88-96
1:400	Average %	87
Range %	84-90
1:800	Average %	95
Range %	91-99
1:1600	Average %	97
Range %	92-102

回收率：

The recovery of human APOC4 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.

Sample Type	Average % Recovery	Range
Serum (n=5)	94	90-98
EDTA plasma (n=4)	98	94-103

标准曲线：

These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

ng/ml	OD1	OD2	Average
400	0.080	0.083	0.082
200	0.117	0.119	0.118
100	0.177	0.172	0.175
50	0.276	0.296	0.286
25	0.504	0.490	0.497
12.5	0.923	0.931	0.927
6.25	1.461	1.496	1.479
0	2.539	2.587	2.563

数据处理：

ELISA操作视频及标曲绘制软件下载
货期：

3-5 working days

功能：

Anticoagulant plasma protein; it is a cofactor to activated protein C in the degradation of coagulation factors Va and VIIIa. It helps to prevent coagulation and stimulating fibrinolysis.
基因功能参考文献：
1. An inhibitory role for PROS1 on BM-derived LCs. Collectively. PMID: 29871951
2. This study identifies a duple role for PROS1 in stem-cell quiescence and as a pro-neurogenic factor, and highlights a unique segregation of increased stem cell proliferation from enhanced neuronal differentiation. PMID: 27753164
3. Mice overexpressing protein S showed significant improvements in blood glucose level, glucose tolerance, insulin sensitivity, and insulin secretion compared with wild-type counterparts. diabetic protein S transgenic mice developed significantly less severe diabetic glomerulosclerosis than controls. PMID: 27207541
4. results support a causal relationship between the PS-K196E mutation and venous thrombosis PMID: 26251307
5. By revealing that neural stem-like cells act within the SVZ neurogenic niche as phagocytes and that the ProS/MerTK path represents an endogenous regulatory mechanism for SVZ cell phagocytic activity PMID: 25308179
6. Data indicate that activated T cells express Pros1. PMID: 23850380
7. Results demonstrate that Protein S is a Mer ligand, and is active in Mer-driven phagocytosis in the retina. PMID: 23259948
8. A self-regulatory mechanism of Toll-like receptor signalling through the suppression of Gas6 and ProS expression is described. PMID: 22043818
9. Protein S controls hypoxic/ischemic blood-brain barrier disruption through the TAM receptor Tyro3 and sphingosine 1-phosphate receptor1. PMID: 20348395
10. Data show that the spectrum of bioactivities of recombinant murine protein S with mouse plasma and smooth muscle cells is similar to that of human protein S. PMID: 19815836
11. results demonstrate that ProS is a pleiotropic anticoagulant with activated Protein C-independent activities and highlight new roles for ProS in vascular development and homeostasis PMID: 19729839
12. Pregnancy causes a decrease in APC resistance in mice, which can be explained by the elevation of protein S levels and increased TFPI activity in plasma. PMID: 19036061
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亚细胞定位：

Secreted.
组织特异性：

Plasma.
数据库链接：

KEGG: mmu:19128

STRING: 10090.ENSMUSP00000023629

UniGene: Mm.127156

Intra-assay Precision (Precision within an assay): CV%<8%

Three samples of known concentration were tested twenty times on one plate to assess.

Inter-assay Precision (Precision between assays): CV%<10%

Three samples of known concentration were tested in twenty assays to assess.