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Mouse Vascular cell adhesion molecule 1,VCAM-1 ELISA kit

  • 中文名称:
    小鼠血管内皮细胞粘附分子1(VCAM-1/CD106)酶联免疫试剂盒
  • 货号:
    CSB-E04754m
  • 规格:
    96T/48T
  • 价格:
    ¥3800/¥2500
  • 其他:

产品详情

  • 产品描述:
    CUSABIO小鼠血管内皮细胞粘附分子1(VCAM-1/CD106)酶联免疫检测试剂盒(货号CSB-E04754m),适用于定量分析血清、血浆、细胞培养上清及组织匀浆样本中的VCAM-1含量。VCAM-1是一种跨膜糖蛋白,主要表达于活化血管内皮细胞表面,通过介导白细胞与内皮细胞粘附参与炎症反应和免疫调控,其异常表达与动脉粥样硬化、肿瘤转移等多种病理过程密切相关。该试剂盒采用双抗体夹心法原理,通过包被于微孔板的高特异性捕获抗体与样本中VCAM-1结合,经生物素标记检测抗体与辣根过氧化物酶(HRP)标记链霉亲和素系统显色,实现39.062-2500 pg/mL的宽泛线性检测范围,灵敏度达39.062 pg/mL,可在4.5小时内完成检测流程。该试剂盒适用于体外研究血管内皮细胞活化状态、炎症反应机制、药物干预效果评估等科研领域,为心血管疾病、免疫调控及肿瘤微环境等领域的机制研究提供可靠工具。
  • 别名:
    Vcam1; Vcam-1; Vascular cell adhesion protein 1; V-CAM 1; VCAM-1; CD antigen CD106
  • 缩写:
  • Uniprot No.:
  • 种属:
    Mus musculus (Mouse)
  • 样本类型:
    serum, plasma, cell culture supernates, tissue homogenates
  • 检测范围:
    39.062 pg/mL-2500 pg/mL
  • 灵敏度:
    29.649 pg/mL
  • 反应时间:
    1-5h
  • 样本体积:
    50-100ul
  • 检测波长:
    450 nm
  • 研究领域:
    Cardiovascular
  • 测定原理:
    quantitative
  • 测定方法:
    Sandwich
  • 精密度:

     

    Intra-assay Precision (Precision within an assay): CV%<8%

    Three samples of known concentration were tested twenty times on one plate to assess.

    Inter-assay Precision (Precision between assays): CV%<10%

    Three samples of known concentration were tested in twenty assays to assess.

     

  • 线性度:

     

    To assess the linearity of the assay, samples were spiked with high concentrations of mouse VCAM-1/CD106 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.

     

  • 标准曲线:

     

    These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

     

  • 数据处理:
  • 货期:
    3-5 working days

产品评价

靶点详情

  • 最新研究进展:
    VCAM1是一种黏附分子,参与细胞黏附和迁移过程,已被证实与许多疾病的发生和发展有关,如动脉粥样硬化和肿瘤的形成。最新的研究表明,VCAM1可能参与心血管疾病的发生和发展,并被认为是一种治疗心血管疾病的潜在靶点。此外,VCAM1的水平还可以作为某些疾病的诊断和预后标志物。
  • 功能:
    Important in cell-cell recognition. Appears to function in leukocyte-endothelial cell adhesion. Interacts with integrin alpha-4/beta-1 (ITGA4/ITGB1) on leukocytes, and mediates both adhesion and signal transduction. The VCAM1/ITGA4/ITGB1 interaction may play a pathophysiologic role both in immune responses and in leukocyte emigration to sites of inflammation.
  • 亚细胞定位:
    [Isoform 1]: Cell membrane; Single-pass type I membrane protein.; [Isoform 2]: Cell membrane; Lipid-anchor, GPI-anchor.
  • 组织特异性:
    Expressed on inflamed vascular endothelium, as well as on macrophage-like and dendritic cell types in both normal and inflamed tissue. Expressed in the bone marrow.
  • 数据库链接: