Mouse Undercarboxylated Osteocalcin (ucOC) ELISA Kit

产品详情

Intra-assay Precision (Precision within an assay): CV%<8%

Three samples of known concentration were tested twenty times on one plate to assess.

Inter-assay Precision (Precision between assays): CV%<10%

Three samples of known concentration were tested in twenty assays to assess.

产品描述：

小鼠羧化不全骨钙素(ucOC)酶联免疫试剂盒（CSB-E17681m）为双抗夹心法ELISA试剂盒，定量检测血清、血浆、组织培养上清液、组织匀浆样本中的ucOC含量。试剂盒检测范围为0.156 ng/mL-10 ng/mL，为骨生物学研究提供可靠工具，特别适用于骨质疏松动物模型构建、骨代谢调控机制研究、药物干预效果评价等科研领域，其优化的检测体系能有效支持血清等复杂样本中微量ucOC的稳定检出，为探索骨骼系统疾病发生发展机制提供数据支撑。本品仅用于科研，不用于临床诊断，产品具体参数及操作步骤详见产品说明书。
缩写：

ucOC
种属：

Mus musculus (Mouse)
样本类型：

serum, plasma, cell culture supernates, tissue homogenates
检测范围：

0.156 ng/mL-10 ng/mL
灵敏度：

0.039 ng/mL
反应时间：

1-5h
样本体积：

50-100ul
检测波长：

450 nm
研究领域：

Others
测定原理：

quantitative
测定方法：

Sandwich

精密度：

线性度：

To assess the linearity of the assay, samples were spiked with high concentrations of mouse ucOC in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
	Sample	Serum(n=4)
1:5	Average %	85
	Range %	80-90
1:10	Average %	92
	Range %	86-96
1:20	Average %	94
	Range %	87-100
1:40	Average %	97
	Range %	92-101

回收率：

The recovery of mouse ucOC spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.

Sample Type	Average % Recovery	Range
Serum (n=5)	93	87-98
EDTA plasma (n=4)	90	87-94

标准曲线：

These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.