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  4. Mouse Tumor necrosis factor receptor superfamily member 17(TNFRSF17) ELISA kit

Mouse Tumor necrosis factor receptor superfamily member 17(TNFRSF17) ELISA kit

说明书下载
  • 中文名称：
    小鼠肿瘤坏死因子配体超家族成员17(TNFSF17)酶联免疫试剂盒
  • 货号：
    CSB-EL023974MO
  • 规格：
    96T/48T
  • 价格：
    ￥3600/￥2500
  • 其他：
CUSABIO Wins 2024 Bioz Rapid Star™ Award in the ELISA category

产品详情

  • 产品描述：
    CUSABIO小鼠肿瘤坏死因子配体超家族成员17（TNFSF17）酶联免疫吸附检测试剂盒（货号：CSB-EL023974MO），采用双抗体夹心法原理实现定量检测，适用于血清、血浆及组织匀浆样本中的TNFSF17蛋白分析。TNFSF17是肿瘤坏死因子超家族的重要成员，主要参与B细胞活化、免疫应答调控及淋巴器官发育等生物学过程，在免疫相关疾病机制研究中具有重要价值。试剂盒检测灵敏度为0.39 ng/ml，线性检测范围为0.39-25 ng/ml，可在标准曲线范围内准确定量目标蛋白浓度。实验操作流程包含预包被捕获抗体、样本孵育、检测抗体结合及显色反应等步骤，支持科研人员高效开展小鼠模型中的免疫调节研究、肿瘤微环境分析或炎症性疾病机制探索，尤其适用于评估实验动物体内TNFSF17表达水平变化与特定病理状态的相关性，为免疫学、分子生物学及药理学研究提供可靠的数据支持。
  • 别名：
    Tnfrsf17; Bcm; Bcma; Tumor necrosis factor receptor superfamily member 17; B-cell maturation protein; CD antigen CD269
  • 缩写：
    TNFRSF17
  • Uniprot No.：
  • 种属：
    Mus musculus (Mouse)
  • 样本类型：
    serum, plasma, tissue homogenates
  • 检测范围：
    0.39 ng/ml - 25 ng/ml.
  • 灵敏度：
    0.1 ng/ml.
  • 反应时间：
    1-5h
  • 样本体积：
    50-100ul
  • 检测波长：
    450 nm
  • 研究领域：
    Immunology
  • 测定原理：
    quantitative
  • 测定方法：
    Sandwich
  • 精密度：

    Intra-assay Precision (Precision within an assay): CV%<8%

    Three samples of known concentration were tested twenty times on one plate to assess.

    Inter-assay Precision (Precision between assays): CV%<10%

    Three samples of known concentration were tested in twenty assays to assess.

  • 线性度：

    To assess the linearity of the assay, samples were spiked with high concentrations of mouse TNFRSF17 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.

    　

    Sample

    Serum(n=4)

    1:1

    Average %

    99

    Range %

    90-104

    1:2

    Average %

    100

    Range %

    90-105

    1:4

    Average %

    96

    Range %

    89-100

    1:8

    Average %

    93

    Range %

    89-97

  • 回收率：

    The recovery of mouse TNFRSF17 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.

    Sample Type

    Average % Recovery

    Range

    Serum (n=5)

    93

    88-97

    EDTA plasma (n=4)

    102

    95-107

  • 标准曲线：

    These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

    ng/ml

    OD1

    OD2

    Average

    Corrected

    25

    2.606

    2.567

    2.587

    2.410

    12.5

    1.832

    1.795

    1.814

    1.637

    6.25

    1.229

    1.200

    1.215

    1.038

    3.125

    0.708

    0.688

    0.698

    0.521

    1.56

    0.425

    0.416

    0.421

    0.244

    0.78

    0.303

    0.289

    0.296

    0.119

    0.39

    0.229

    0.232

    0.231

    0.054

    0

    0.179

    0.175

    0.177

    　

  • 数据处理：
    ELISA操作视频及标曲绘制软件下载
  • 货期：
    3-5 working days

产品评价

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Tnfrsf17 Proteins

靶点详情

  • 功能：
    Receptor for TNFSF13B/BLyS/BAFF and TNFSF13/APRIL. Promotes B-cell survival and plays a role in the regulation of humoral immunity. Activates NF-kappa-B and JNK.
  • 基因功能参考文献：
    1. high affinity antibody blocks the binding of the native ligands APRIL and BAFF to BCMA. This finding is rationalized by the high resolution crystal structure of the Fab fragment in complex with the extracellular domain of BCMA PMID: 25953704
    2. Elimination of B lymphocyte stimulator receptor 3 (BR-3) and BCMA inhibits the development of SLE in NZM mice. PMID: 25989238
    3. we have identified a novel role for BCMA to control excess BAFF production in murine lupus through restraining the accumulation of BAFF-producing neutrophils. PMID: 25010693
    4. Heteromers consisting of one BAFF and two APRIL bind to receptor TACI, BCMA but not to BAFFR. PMID: 25953898
    5. BCMA is dispensable for the development of SLE in NZM mice. BAFF-BCMA interactions contribute to SLE. PMID: 23334904
    6. Study reveals that BCMA works to control B cell homeostasis and self-tolerance in systemic autoimmunity. PMID: 21536804
    7. Blimp-1 is a positive regulator of the expression of BCMA gene in mouse plasma cells. PMID: 20339926
    8. The 5'-flanking sequence from -820 to +145 bp of BCMA is of promoter activity. PMID: 18687216
    9. A vital role of BCMA in bone marrow plasma cell survival. PMID: 14707116
    10. B cell maturation antigen, the receptor for a proliferation-inducing ligand and B cell-activating factor of the TNF family, induces antigen presentation in B cells. PMID: 16116167
    11. Review. APRIL interactions with BCMA likely govern memory B cell populations. PMID: 16919470
    12. Review. Direct BAFF/APRIL signalling in T cells and/or T cell modulation in response to a BAFF-modified B cell compartment may play an important role in inflammation and immunomodulation. PMID: 16931039

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  • 亚细胞定位：
    Membrane; Single-pass type III membrane protein.
  • 组织特异性：
    Detected in spleen, thymus, bone marrow and heart, and at lower levels in kidney and lung.
  • 数据库链接：

    KEGG: mmu:21935

    STRING: 10090.ENSMUSP00000023140

    UniGene: Mm.12935

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九游平台-九游(中国) | 米兰体育-米兰体育（中国） | 开云手机在线登陆入口 | 江南官方体育网页版 | 开云网页版 | 开云官方在线入口 | 开云电子体育 | 开云官方链接 | 江南网页版页面登录 |