Mouse Transforming Growth factor β3,TGF-β3 ELISA kit

Mouse Transforming Growth factor β3,TGF-β3 ELISA kit

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中文名称：

小鼠转化生长因子β3(TGF-β3)酶联免疫试剂盒
货号：

CSB-E12862m
规格：

96T/48T
价格：

￥3200/￥2500
其他：

CUSABIO Wins 2024 Bioz Rapid Star™ Award in the ELISA category

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产品描述：

小鼠转化生长因子β3(TGF-β3)酶联免疫试剂盒（CSB-E12862m）为双抗夹心法ELISA试剂盒，定量检测血清、血浆、组织培养上清液、组织匀浆样本中的TGFB3含量。TGFB3即转化生长因子β3，在胚胎发育、组织修复等多种生理过程中扮演重要角色。其研究机制主要围绕调节细胞生长、分化、黏附及凋亡等方面。异常表达与肿瘤发生发展、纤维化疾病等密切相关，对其深入研究有望为相关疾病治疗提供新策略。试剂盒检测范围为125 pg/mL-8000 pg/mL，本试剂盒适用于体外实验研究，可广泛用于探究TGF-β3在细胞功能调控、炎症或纤维化动物模型中的动态变化，亦可用于评估体外培养细胞分泌TGF-β3的能力，为研究组织再生、疾病机制及药物干预效果提供可靠的科研工具。本品仅用于科研，不用于临床诊断，产品具体参数及操作步骤详见产品说明书。
别名：

Tgfb3 ELISA Kit; Transforming growth factor beta-3 proprotein [Cleaved into: Latency-associated peptide ELISA Kit; LAP); Transforming growth factor beta-3 ELISA Kit; TGF-beta-3)] ELISA Kit
缩写：

TGFB3
Uniprot No.：

P17125
种属：

Mus musculus (Mouse)
样本类型：

serum, plasma, cell culture supernates, tissue homogenates
检测范围：

125 pg/mL-8000 pg/mL
灵敏度：

31.25 pg/mL
反应时间：

1-5h
样本体积：

50-100ul
检测波长：

450 nm
研究领域：

Signal Transduction
测定原理：

quantitative
测定方法：

Sandwich

精密度：

线性度：

To assess the linearity of the assay, samples were spiked with high concentrations of mouse TGF-β3 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
	Sample	Serum(n=4)
1:1	Average %	93
	Range %	89-97
1:2	Average %	95
	Range %	92-102
1:4	Average %	96
	Range %	93-102
1:8	Average %	94
	Range %	89-99

回收率：

The recovery of mouse TGF-β3 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.

Sample Type	Average % Recovery	Range
Serum (n=5)	95	90-99
EDTA plasma (n=4)	91	84-98

标准曲线：

These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

pg/ml	OD1	OD2	Average	Corrected
8000	2.646	2.815	2.731	2.542
4000	2.016	2.155	2.086	1.897
2000	1.195	1.264	1.230	1.041
1000	0.778	0.798	0.788	0.599
500	0.493	0.506	0.500	0.311
250	0.336	0.361	0.349	0.160
125	0.279	0.265	0.272	0.083
0	0.192	0.185	0.189

数据处理：

ELISA操作视频及标曲绘制软件下载
货期：

3-5 working days

Approaching Thrombospondin-1 as a Potential Target for Mesenchymal Stromal Cells to Support Liver Regeneration after Partial Hepatectomy in Mouse and Humans L Tietze,Cells,2024
Prenatal and Postnatal Exposure to Polystyrene Microplastics Induces Testis Developmental Disorder and Affects Male Fertility in Mice T Zhao,Journal of Hazardous Materials,2022

功能：

Transforming growth factor beta-3 proprotein: Precursor of the Latency-associated peptide (LAP) and Transforming growth factor beta-3 (TGF-beta-3) chains, which constitute the regulatory and active subunit of TGF-beta-3, respectively.; Required to maintain the Transforming growth factor beta-3 (TGF-beta-3) chain in a latent state during storage in extracellular matrix. Associates non-covalently with TGF-beta-3 and regulates its activation via interaction with 'milieu molecules', such as LTBP1 and LRRC32/GARP, that control activation of TGF-beta-3. Interaction with integrins results in distortion of the Latency-associated peptide chain and subsequent release of the active TGF-beta-3.; Transforming growth factor beta-3: Multifunctional protein that regulates embryogenesis and cell differentiation and is required in various processes such as secondary palate development. Activation into mature form follows different steps: following cleavage of the proprotein in the Golgi apparatus, Latency-associated peptide (LAP) and Transforming growth factor beta-3 (TGF-beta-3) chains remain non-covalently linked rendering TGF-beta-3 inactive during storage in extracellular matrix. At the same time, LAP chain interacts with 'milieu molecules', such as LTBP1 and LRRC32/GARP that control activation of TGF-beta-3 and maintain it in a latent state during storage in extracellular milieus. TGF-beta-3 is released from LAP by integrins: integrin-binding results in distortion of the LAP chain and subsequent release of the active TGF-beta-3. Once activated following release of LAP, TGF-beta-3 acts by binding to TGF-beta receptors (TGFBR1 and TGFBR2), which transduce signal.
亚细胞定位：

[Latency-associated peptide]: Secreted, extracellular space, extracellular matrix.; [Transforming growth factor beta-3]: Secreted.
蛋白家族：

TGF-beta family
数据库链接：

STRING: 10090.ENSMUSP00000003687

UniGene: Mm.3992

Intra-assay Precision (Precision within an assay): CV%<8%

Three samples of known concentration were tested twenty times on one plate to assess.

Inter-assay Precision (Precision between assays): CV%<10%

Three samples of known concentration were tested in twenty assays to assess.