Mouse Thyroid-Peroxidase,TPO ELISA Kit

产品详情

产品描述：

CUSABIO小鼠甲状腺过氧化物酶（TPO）定量检测试剂盒（货号CSB-E08353m），基于竞争法原理设计，适用于科研领域检测血清、血浆、细胞培养上清及组织匀浆样本中的TPO含量。TPO是甲状腺激素合成的关键酶，负责催化碘化酪氨酸残基的氧化及甲状腺球蛋白的偶联反应，其表达水平与甲状腺功能状态密切相关。试剂盒采用标准化的定量检测流程，检测灵敏度达2.5 ng/mL，线性范围覆盖2.5-40 ng/mL，可在科研实验中精准分析不同样本类型的TPO浓度变化。该产品特别适用于甲状腺功能调控机制研究、自身免疫性甲状腺疾病模型建立、药物或环境因素对甲状腺代谢影响的评估等场景，为探索甲状腺相关分子机制提供可靠工具。实验过程中，样本中的TPO会与酶标记物竞争结合特异性抗体，通过检测显色信号强度与标准曲线的比对实现定量分析，确保数据准确性和重复性。
别名：

Tpo ELISA Kit; Thyroid peroxidase ELISA Kit; TPO ELISA Kit; EC 1.11.1.8 ELISA Kit
缩写：

TPO
Uniprot No.：

P35419
种属：

Mus musculus (Mouse)
样本类型：

serum, plasma, cell culture supernates, tissue homogenates
检测范围：

2.5 ng/mL-40 ng/mL
灵敏度：

2.5 ng/mL
反应时间：

1-5h
样本体积：

50-100ul
检测波长：

450 nm
研究领域：

Cancer
测定原理：

quantitative
测定方法：

Competitive

精密度：

Intra-assay Precision (Precision within an assay): CV%<15%
Three samples of known concentration were tested twenty times on one plate to assess.
Inter-assay Precision (Precision between assays): CV%<15%
Three samples of known concentration were tested in twenty assays to assess.

线性度：

To assess the linearity of the assay, samples were spiked with high concentrations of mouse TPO in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
	Sample	Serum(n=4)
1:1	Average %	90
	Range %	86-103
1:2	Average %	89
	Range %	82-97
1:4	Average %	95
	Range %	87-100
1:8	Average %	90
	Range %	83-99

回收率：

The recovery of mouse TPO spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.

Sample Type	Average % Recovery	Range
Serum (n=5)	94	87-102
EDTA plasma (n=4)	95	90-103

标准曲线：

These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

功能：

Iodination and coupling of the hormonogenic tyrosines in thyroglobulin to yield the thyroid hormones T(3) and T(4).
基因功能参考文献：
1. Lnk Deficiency Leads to TPO-Mediated Osteoclastogenesis and Increased Bone Mass Phenotype PMID: 28067429
2. Hearing impairment in hypothyroid dwarf mice caused by mutations of the thyroid peroxidase gene. PMID: 24297261
3. A novel hypothyroid dwarfism is due to the missense mutation Arg479Cys of the thyroid peroxidase gene in the mouse. PMID: 16762971
亚细胞定位：

Membrane; Single-pass type I membrane protein.
蛋白家族：

Peroxidase family, XPO subfamily
数据库链接：

KEGG: mmu:22018

STRING: 10090.ENSMUSP00000021005

UniGene: Mm.4991