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  4. Mouse Thyroglobulin,TG ELISA Kit

Mouse Thyroglobulin,TG ELISA Kit

文献引用 (4) 说明书下载
  • 中文名称：
    小鼠甲状腺球蛋白(TG)酶联免疫试剂盒
  • 货号：
    CSB-E08241m
  • 规格：
    96T/48T
  • 价格：
    ￥3800/￥2500
  • 其他：
CUSABIO Wins 2024 Bioz Rapid Star™ Award in the ELISA category

产品详情

  • 产品描述：
    本试剂盒专为定量检测小鼠血清、血浆及组织匀浆样本中的甲状腺球蛋白（Thyroglobulin, TG）而设计，该蛋白是甲状腺激素合成的关键前体，在甲状腺激素储存与释放中发挥核心作用，其表达水平与甲状腺功能状态密切相关。本产品采用竞争法酶联免疫吸附测定原理，检测灵敏度覆盖10 ng/mL至160 ng/mL的浓度区间，可精准量化低丰度样本中的TG含量。实验操作兼容多种生物基质，包括血清、血浆及组织匀浆（需离心预处理），适用于甲状腺疾病模型研究、自身免疫性甲状腺炎机制探索、甲状腺代谢调控实验等科研场景。试剂盒提供预包被抗体与优化的反应体系，确保检测稳定性和特异性，为甲状腺相关分子机制研究及药物干预效果评估提供可靠工具。
  • 别名：
    Tg ELISA Kit; Tgn ELISA Kit; Thyroglobulin ELISA Kit; Tg ELISA Kit
  • 缩写：
    TG
  • Uniprot No.：
  • 种属：
    Mus musculus (Mouse)
  • 样本类型：
    serum, plasma, tissue homogenates
  • 检测范围：
    10 ng/mL-160 ng/mL
  • 灵敏度：
    10 ng/mL
  • 反应时间：
    1-5h
  • 样本体积：
    50-100ul
  • 检测波长：
    450 nm
  • 研究领域：
    Metabolism
  • 测定原理：
    quantitative
  • 测定方法：
    Competitive
  • 精密度：
    Intra-assay Precision (Precision within an assay): CV%<8%        
    Three samples of known concentration were tested twenty times on one plate to assess.    
    Inter-assay Precision (Precision between assays): CV%<10%        
    Three samples of known concentration were tested in twenty assays to assess.      
                   
  • 线性度：
    To assess the linearity of the assay, samples were spiked with high concentrations of mouse TG in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.  
    　 Sample Serum(n=4)    
    1:1 Average % 90    
    Range % 85-100    
    1:2 Average % 89    
    Range % 82-100    
    1:4 Average % 93    
    Range % 88-100    
    1:8 Average % 95    
    Range % 89-105    
  • 回收率：
    The recovery of mouse TG spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.  
     
    Sample Type Average % Recovery Range    
    Serum (n=5) 97 92-110    
    EDTA plasma (n=4) 95 89-100    
                   
                   
  • 标准曲线：
    These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.  
     
    ng/ml OD1 OD2 Average 　    
    160 0.165 0.170 0.168 　    
    80 0.276 0.271 0.274 　    
    40 0.504 0.499 0.502 　    
    20 1.008 1.034 1.021 　    
    10 2.055 2.029 2.042 　    
  • 数据处理：
    ELISA操作视频及标曲绘制软件下载
  • 货期：
    3-5 working days

引用文献

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Tg Proteins

靶点详情

  • 功能：
    Acts as a substrate for the production of iodinated thyroid hormones thyroxine (T4) and triiodothyronine (T3). The synthesis of T3 and T4 involves iodination of selected tyrosine residues of TG/thyroglobulin followed by their oxidative coupling. Following TG re-internalization and lysosomal-mediated proteolysis, T3 and T4 are released from the polypeptide backbone leading to their secretion into the bloodstream. One dimer produces 7 thyroid hormone molecules.
  • 基因功能参考文献：
    1. Findings suggest that coumestrol treatment may have some beneficial actions against thyroid-specific thyroglobulin (Tg) autoantibody production in the development of autoimmune thyroiditis through suppression of Th1 response. PMID: 27384679
    2. these data identify for the first time a Tg T cell epitope as a spontaneous target in ISAT. PMID: 24879795
    3. Final acquisition of secretory competence includes conformational maturation in the interval between linker and hinge segments of region I of thyroglobulin. PMID: 21816825
    4. The results showed that thyroglobulin message or protein is not expressed in placenta, decidua, or ovary in any stages of pregnancy. PMID: 20236260
    5. Findings demonstrate a novel aspect of murine and human thyroid autoimmunity, namely breaking B cell self-tolerance occurs first for Tg and subsequently for TPO. PMID: 20573721
    6. These results indicate that Runx2 deficiency in mice causes decreased Tg expression and a novel type of hypothyroidism. PMID: 20375239
    7. Development of iodine-accelerated autoimmune thyroiditis in NOD.H2h4 mice is not associated with enhanced iodine organification or differential B- or T-cell responses to iodinated determinants in Tg. PMID: 20687399
    8. specific properties of distinct Tg ChEL mutants may result in distinct patterns of Tg monomer folding PMID: 20353937
    9. Five novel autoimmune thyroiditis-causing T cell epitopes of thyroglobulin (Tgn) have been delineated and mapped with an algorithm that predicts Tgn immunopathogenic peptides containing I-A(k)-binding motifs. PMID: 12391254
    10. iodotyrosyl formation in normal thyroglobulin confers pathogenic potential to certain peptides that may otherwise remain innocuous and undetectable by conventional mapping methods PMID: 16547286
    11. Tg could initiate glomerular injury by reproducing the actions of TGF-beta1 in the mesangial cell. PMID: 17495424
    12. Data indicate that the R19K mutation induces local misfolding in the amino-terminal domain of Tg that has global effects on Tg transport and thyroid hormonogenesis. PMID: 17916655
    13. Together these results suggest that a unique low molecular weight variant of Tg is expressed in the kidney, where it could serve both physiological and pathological roles, including that of an autoantigen. PMID: 19716808

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  • 相关疾病：
    Defects in Tg are the cause of some forms of goiter. Goiter is an enlargement of the thyroid gland. The variant Pro-2283 exhibits a defect in exit from the endoplasmic reticulum.
  • 亚细胞定位：
    Secreted.
  • 蛋白家族：
    Type-B carboxylesterase/lipase family
  • 组织特异性：
    Specifically expressed in the thyroid gland.
  • 数据库链接：

    KEGG: mmu:21819

    STRING: 10090.ENSMUSP00000070239

    UniGene: Mm.441333

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九游平台-九游(中国) | 米兰体育-米兰体育（中国） | 开云手机在线登陆入口 | 江南官方体育网页版 | 开云网页版 | 开云官方在线入口 | 开云电子体育 | 开云官方链接 | 江南网页版页面登录 |