Mouse Spondin-1(SPON1) ELISA kit

产品详情

产品描述：

小鼠脊椎蛋白-1(SPON1)酶联免疫试剂盒（CSB-EL022599MO）为双抗夹心法ELISA试剂盒，定量检测血清、血浆、组织匀浆样本中的SPON1含量。小鼠脊椎蛋白-1（SPON1）是一种在脊椎动物中广泛表达的蛋白质，参与细胞外基质构建和细胞粘附。其研究涉及细胞信号传导、组织修复和肿瘤发生等机制，对理解脊椎发育和疾病治疗具有重要意义。试剂盒检测范围为0.156 ng/mL-10 ng/mL，适用于实验室环境下的小鼠模型研究，如探究骨骼发育、神经系统疾病或肿瘤微环境中SPON1的调控机制，亦可应用于基因编辑动物或药物干预实验中生物标志物的动态监测本品仅用于科研，不用于临床诊断，产品具体参数及操作步骤详见产品说明书。
别名：

Spon1 ELISA Kit; Spondin-1 ELISA Kit; F-spondin ELISA Kit
缩写：

SPON1
Uniprot No.：

Q8VCC9
种属：

Mus musculus (Mouse)
样本类型：

serum, plasma, tissue homogenates
检测范围：

0.156 ng/mL-10 ng/mL
灵敏度：

0.039 ng/mL
反应时间：

1-5h
样本体积：

50-100ul
检测波长：

450 nm
研究领域：

Neuroscience
测定原理：

quantitative
测定方法：

Sandwich

精密度：

Intra-assay Precision (Precision within an assay): CV%<8%
Three samples of known concentration were tested twenty times on one plate to assess.
Inter-assay Precision (Precision between assays): CV%<10%
Three samples of known concentration were tested in twenty assays to assess.

线性度：

To assess the linearity of the assay, samples were spiked with high concentrations of mouse SPON1 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
	Sample	Serum(n=4)
1:1	Average %	102
	Range %	97-106
1:2	Average %	104
	Range %	99-108
1:4	Average %	86
	Range %	81-92
1:8	Average %	94
	Range %	89-98

回收率：

The recovery of mouse SPON1 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.

Sample Type	Average % Recovery	Range
Serum (n=5)	102	97-106
EDTA plasma (n=4)	87	83-92

标准曲线：

These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

功能：

Cell adhesion protein that promotes the attachment of spinal cord and sensory neuron cells and the outgrowth of neurites in vitro. May contribute to the growth and guidance of axons in both the spinal cord and the PNS.
基因功能参考文献：
1. We conclude that upon ligand binding, TLR4 activates PI3K/Akt signaling to induce F-spondin expression, subsequently control CREB-mediated IL-6 production to promote VSMC migration. These findings provide vital insights into the essential role of F-spondin in VSMC function and will be valuable for developing new therapeutic strategies against atherosclerosis. PMID: 27091427
2. These findings demonstrated novel roles for F-spondin and IL-1Ra in type B CpG oligodeoxynucleotides-mediated cell proliferation and migration of macrophages. PMID: 26042735
3. Spon1 deletion leads to a local and systemic reduction of TGF-beta levels resulting in increased BMP signaling and increased bone deposition in adult mice. PMID: 24875054
4. F-spondin is expressed in embryonic cartilage, where it has the capacity to enhance chondrocyte terminal differentiation and mineralization. PMID: 20839318
亚细胞定位：

Secreted, extracellular space, extracellular matrix.
数据库链接：

KEGG: mmu:233744

STRING: 10090.ENSMUSP00000041157

UniGene: Mm.334160