Mouse Signal peptide, CUB and EGF-like domain-containing protein 1(SCUBE1) ELISA kit

Mouse Signal peptide, CUB and EGF-like domain-containing protein 1(SCUBE1) ELISA kit

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中文名称：

小鼠信号肽、CUB和EGF样结构域蛋白1(SCUBE1)酶联免疫试剂盒
货号：

CSB-EL020866MO
规格：

96T/48T
价格：

￥3600/￥2500
其他：

CUSABIO Wins 2024 Bioz Rapid Star™ Award in the ELISA category

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产品描述：

小鼠信号肽、CUB和EGF样结构域蛋白1（SCUBE1）酶联免疫试剂盒（货号：CSB-EL020866MO）是一款基于双抗体夹心法原理设计的定量检测试剂盒，适用于检测小鼠血清、血浆及组织匀浆样本中的SCUBE1蛋白水平。SCUBE1是一种多功能跨膜糖蛋白，参与调控细胞黏附、信号传导及组织发育等生物学过程，在心血管系统、骨骼发育及肿瘤微环境中具有潜在研究价值。本试剂盒检测灵敏度高，线性范围为0.156 ng/mL至10 ng/mL，可精准覆盖低浓度样本的检测需求。实验操作采用预包被特异性抗体的微孔板，通过标准品与样本中SCUBE1蛋白竞争结合标记抗体，结合显色反应实现定量分析。该产品适用于基础科研领域，如探究SCUBE1在疾病模型中的表达调控机制、评估基因敲除或药物干预后的蛋白水平变化，以及作为相关信号通路研究的生物标志物检测工具。试剂盒配套提供标准品、检测抗体及优化缓冲液体系，确保实验的稳定性和重复性，满足体外实验中对SCUBE1蛋白定量分析的需求。
别名：

Scube1 ELISA Kit; Signal peptide ELISA Kit; CUB and EGF-like domain-containing protein 1 ELISA Kit
缩写：

SCUBE1
Uniprot No.：

Q6NZL8
种属：

Mus musculus (Mouse)
样本类型：

serum, plasma, tissue homogenates
检测范围：

0.156 ng/mL-10 ng/mL
灵敏度：

0.039 ng/mL
反应时间：

1-5h
样本体积：

50-100ul
检测波长：

450 nm
研究领域：

Cardiovascular
测定原理：

quantitative
测定方法：

Sandwich

精密度：

线性度：

To assess the linearity of the assay, samples were spiked with high concentrations of mouse SCUBE1 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
	Sample	Serum(n=4)
1:5	Average %	92
	Range %	88-95
1:10	Average %	101
	Range %	96-104
1:20	Average %	92
	Range %	87-95
1:40	Average %	98
	Range %	92-101

回收率：

The recovery of mouse SCUBE1 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.

Sample Type	Average % Recovery	Range
Serum (n=5)	92	87-97
EDTA plasma (n=4)	107	99-111

标准曲线：

These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

ng/ml	OD1	OD2	Average	Corrected
10	2.871	2.775	2.823	2.711
5	2.054	1.989	2.022	1.910
2.5	1.123	1.138	1.131	1.019
1.25	0.645	0.675	0.660	0.548
0.625	0.372	0.384	0.378	0.266
0.312	0.287	0.277	0.282	0.170
0.156	0.196	0.189	0.193	0.081
0	0.113	0.111	0.112

数据处理：

ELISA操作视频及标曲绘制软件下载
货期：

3-5 working days

功能：

Could function as an adhesive molecule and its matrix bound and soluble fragments may play a critical role in vascular biology.
基因功能参考文献：
1. SCUBE1 participates in platelet aggregation by bridging adjacent activated platelets in thrombosis. Blockade of soluble SCUBE1 might represent a novel antithrombotic strategy. PMID: 24833801
2. SCUBE1 may be involved in the regulation of tubular cell proliferation and re-epithelialization during the resolution of kidney injury. PMID: 20042401
3. These results support the dual roles of SCUBE1 on brain morphogenesis and cell-cell adhesions through its distinct domain function. PMID: 18303018
4. During tooth development, Scube1 localized to the dental papilla of both incisor and molar teeth, suggestina a role for Scube1 during early craniofacial development. PMID: 19101956
亚细胞定位：

Secreted. Cell membrane; Peripheral membrane protein.
数据库链接：

KEGG: mmu:64706

UniGene: Mm.40393

Intra-assay Precision (Precision within an assay): CV%<8%

Three samples of known concentration were tested twenty times on one plate to assess.

Inter-assay Precision (Precision between assays): CV%<10%

Three samples of known concentration were tested in twenty assays to assess.