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  4. Mouse Roundabout homolog 4(ROBO4) ELISA kit

Mouse Roundabout homolog 4(ROBO4) ELISA kit

文献引用 (1) 说明书下载
  • 中文名称：
    小鼠环形交叉轴突导向受体同源物4(ROBO4)酶联免疫试剂盒
  • 货号：
    CSB-EL020057MO
  • 规格：
    96T/48T
  • 价格：
    ￥3600/￥2500
  • 其他：
CUSABIO Wins 2024 Bioz Rapid Star™ Award in the ELISA category

产品详情

  • 产品描述：
    小鼠环形交叉轴突导向受体同源物4(ROBO4)酶联免疫试剂盒（CSB-EL020057MO）为双抗夹心法ELISA试剂盒，定量检测血清、血浆、组织匀浆样本中的ROBO4含量。ROBO4是一个重要靶点。其在血管生成与稳定性维持等方面具关键作用。研究机制上，它能参与调节内皮细胞的行为和功能，影响血管的发育和重塑，在肿瘤血管生成等病理过程的研究中受到关注，或为相关疾病治疗提供新方向。试剂盒检测范围为0.156 ng/mL-10 ng/mL，适用于体外培养模型、动物实验样本中ROBO4的动态监测；广泛用于血管生物学、肿瘤微环境、缺血性疾病及炎症相关机制的基础研究，为探索内皮细胞功能调控及病理生理过程提供可靠工具。本品仅用于科研，不用于临床诊断，产品具体参数及操作步骤详见产品说明书。
  • 别名：
    Robo4 ELISA Kit; Roundabout homolog 4 ELISA Kit
  • 缩写：
    ROBO4
  • Uniprot No.：
  • 种属：
    Mus musculus (Mouse)
  • 样本类型：
    serum, plasma, tissue homogenates
  • 检测范围：
    0.156 ng/mL-10 ng/mL
  • 灵敏度：
    0.039 ng/mL
  • 反应时间：
    1-5h
  • 样本体积：
    50-100ul
  • 检测波长：
    450 nm
  • 研究领域：
    Cancer
  • 测定原理：
    quantitative
  • 测定方法：
    Sandwich
  • 精密度：
    Intra-assay Precision (Precision within an assay): CV%<8%
    Three samples of known concentration were tested twenty times on one plate to assess.
    Inter-assay Precision (Precision between assays): CV%<10%
    Three samples of known concentration were tested in twenty assays to assess.
  • 线性度：
    To assess the linearity of the assay, samples were spiked with high concentrations of mouse ROBO4 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
    　 Sample Serum(n=4)
    1:1 Average % 86
    Range % 81-89
    1:2 Average % 98
    Range % 94-103
    1:4 Average % 86
    Range % 83-89
    1:8 Average % 98
    Range % 95-102
  • 回收率：
    The recovery of mouse ROBO4 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.
    Sample Type Average % Recovery Range
    Serum (n=5) 104 101-109
    EDTA plasma (n=4) 89 85-93
  • 标准曲线：
    These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.
    ng/ml OD1 OD2 Average Corrected
    10 2.524 2.631 2.578 2.404
    5 1.957 1.904 1.931 1.757
    2.5 1.240 1.304 1.272 1.098
    1.25 0.753 0.768 0.761 0.587
    0.625 0.496 0.511 0.504 0.330
    0.312 0.302 0.320 0.311 0.137
    0.156 0.220 0.234 0.227 0.053
    0 0.176 0.172 0.174 　
  • 数据处理：
    ELISA操作视频及标曲绘制软件下载
  • 货期：
    3-5 working days

引用文献

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Robo4 Proteins

靶点详情

  • 功能：
    Receptor for Slit proteins, at least for SLIT2, and seems to be involved in angiogenesis and vascular patterning. May mediate the inhibition of primary endothelial cell migration by Slit proteins. Involved in the maintenance of endothelial barrier organization and function.
  • 基因功能参考文献：
    1. Robo4 inhibits angiogenesis and vessel permeability independently of its cytoplasmic domain. Robo4 deletion enhances permeability and revascularization in oxygen-induced retinopathy (OIR) and accelerates cutaneous wound healing. PMID: 27882935
    2. endothelial Robo4 signaling is important for suppressing breast cancer growth and metastasis, and it can be targeted (enhanced) by administrating a small molecular drug. PMID: 26778715
    3. Robo4 vaccines induce antibodies that retard tumor growth. PMID: 25348086
    4. Robo4 and Src family kinases are involved in the mechanisms of embryonic vascular defects caused by the Hrt/Hey deficiency. PMID: 25264302
    5. These results clearly indicate that Robo4 plays a role in hematopoietic stem cells trafficking such as bone marrow homing and peripheral mobilization. PMID: 23226403
    6. our results showed an important role of Robo4 in the regulation of cortical radial migration through Slit-dependent and -independent mechanisms PMID: 22123939
    7. Data found that expression of Slit2 and SDF1 were dynamically regulated in mesenchymal stem cells and OBC-like populations following radiation, while Robo4 expression was restricted to endothelial cells. PMID: 22313734
    8. augmenting the host Robo4/Slit2 system could provide a useful therapeutic approach to control pathological angiogenesis associated with HSV induced stromal keratitis PMID: 21572022
    9. Robo4 specifically binds to UNC5B, a vascular Netrin receptor, revealing unexpected interactions between two endothelial guidance receptors. PMID: 21238923
    10. Activating with the soluble ligand Slit an endothelium-specific, Robo4-dependent signaling pathway that strengthens the vascular barrier, diminishing deleterious aspects of the host's response to the pathogen-induced cytokine storm. PMID: 20375003
    11. enhanced growth of blood vessels in Robo4(-/-) endothelium is due to activation of vascular endothelial growth factor (VEGF)-R2 signaling through the Src and FAK kinases. PMID: 20498081
    12. Robo4 is a bona fide member of the Robo family and may provide a repulsive cue to migrating endothelial cells during vascular development. PMID: 12941633
    13. Robo4 is expressed in hematopoietic stem cells (HSC), and Robo4/Slit2 signaling may play a role in HSC homeostasis in the bone marrow niche. PMID: 18927479
    14. Robo4 mRNA level decreases during vascular decline in pulmonary fibrosis. PMID: 17496152

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  • 蛋白家族：
    Immunoglobulin superfamily, ROBO family
  • 组织特异性：
    Expressed specifically in embryo and adult vascular endothelium.
  • 数据库链接：

    KEGG: mmu:74144

    STRING: 10090.ENSMUSP00000099959

    UniGene: Mm.485070

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九游平台-九游(中国) | 米兰体育-米兰体育（中国） | 开云手机在线登陆入口 | 江南官方体育网页版 | 开云网页版 | 开云官方在线入口 | 开云电子体育 | 开云官方链接 | 江南网页版页面登录 |