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  4. Mouse Regenerating islet-derived protein 3-alpha(REG3A) ELISA kit

Mouse Regenerating islet-derived protein 3-alpha(REG3A) ELISA kit

文献引用 (1) 说明书下载
  • 中文名称：
    小鼠再生胰岛衍生蛋白3α(REG3A）酶联免疫试剂盒
  • 货号：
    CSB-EL019548MO
  • 规格：
    96T/48T
  • 价格：
    ￥3600/￥2500
  • 其他：
CUSABIO Wins 2024 Bioz Rapid Star™ Award in the ELISA category

产品详情

  • 产品描述：
    小鼠再生胰岛衍生蛋白3α(REG3A）酶联免疫试剂盒（CSB-EL019548MO）为双抗夹心法ELISA试剂盒，定量检测血清、血浆、组织匀浆样本中的REG3A含量。REG3A作为一个靶点在研究中备受关注。它在机体的多种生理和病理过程中发挥作用。研究机制上，主要聚焦于其在免疫调节、组织修复及肿瘤发生发展等方面的功能，揭示其信号通路及与其他分子的相互作用，为相关疾病治疗提供思路。试剂盒检测范围为3.12 ng/mL-200 ng/mL，适用于研究REG3A在糖尿病动物模型中的动态变化、肠道炎症机制探索或组织修复过程中的调控作用，为疾病机制研究、药物疗效评估及分子通路分析提供可靠工具本品仅用于科研，不用于临床诊断，产品具体参数及操作步骤详见产品说明书。
  • 别名：
    Reg3a ELISA Kit; Pap2 ELISA Kit; Regenerating islet-derived protein 3-alpha ELISA Kit; REG-3-alpha ELISA Kit; Islet of Langerhans regenerating protein 3 ELISA Kit; Lithostathine 3 ELISA Kit; Pancreatitis-associated protein 2 ELISA Kit; Regenerating islet-derived protein III-alpha ELISA Kit; Reg III-alpha) [Cleaved into: Regenerating islet-derived protein 3-alpha 16.5 kDa form; Regenerating islet-derived protein 3-alpha 15 kDa form] ELISA Kit
  • 缩写：
    REG3A
  • Uniprot No.：
  • 种属：
    Mus musculus (Mouse)
  • 样本类型：
    serum, plasma, tissue homogenates
  • 检测范围：
    3.12 ng/mL-200 ng/mL
  • 灵敏度：
    0.78 ng/mL
  • 反应时间：
    1-5h
  • 样本体积：
    50-100ul
  • 检测波长：
    450 nm
  • 研究领域：
    Immunology
  • 测定原理：
    quantitative
  • 测定方法：
    Sandwich
  • 精密度：
    Intra-assay Precision (Precision within an assay): CV%<8%
    Three samples of known concentration were tested twenty times on one plate to assess.
    Inter-assay Precision (Precision between assays): CV%<10%
    Three samples of known concentration were tested in twenty assays to assess.
  • 线性度：
    To assess the linearity of the assay, samples were spiked with high concentrations of mouse REG3A in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
    　 Sample Serum(n=4)
    1:1 Average % 102
    Range % 98-106
    1:2 Average % 98
    Range % 96-104
    1:4 Average % 88
    Range % 86-95
    1:8 Average % 96
    Range % 91-102
  • 回收率：
    The recovery of mouse REG3A spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.
    Sample Type Average % Recovery Range
    Serum (n=5) 94 90-97
    EDTA plasma (n=4) 88 84-93
  • 标准曲线：
    These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.
    ng/ml OD1 OD2 Average Corrected
    200 2.360 2.512 2.436 2.311
    100 1.831 1.987 1.909 1.784
    50 1.252 1.254 1.253 1.128
    25 0.747 0.766 0.757 0.632
    12.5 0.473 0.512 0.493 0.368
    6.25 0.315 0.321 0.318 0.193
    3.12 0.219 0.217 0.218 0.093
    0 0.124 0.126 0.125 　
  • 数据处理：
    ELISA操作视频及标曲绘制软件下载
  • 货期：
    3-5 working days

引用文献

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Reg3a Proteins

靶点详情

  • 功能：
    Bactericidal C-type lectin. Regulates keratinocyte proliferation and differentiation after skin injury via activation of EXTL3-PI3K-AKT signaling pathway.
  • 基因功能参考文献：
    1. skin injury increases IL-36gamma via the activation of TLR3-SLUG-VDR axis and IL-36gamma induces REG3A to promote wound healing PMID: 28774595
    2. Reg3alpha is an extracellular matrix-targeted reactive oxygen species scavenger that binds the fibrin scaffold resulting from hepatocyte death during acute liver failure. PMID: 25938566
    3. Data show that regenerating islet-derived 3-alpha (REG3alpha) is a plasma biomarker of gastrointestinal tract graft-versus-host disease that can be combined with clinical stage and histologic grade to improve risk stratification of patients. PMID: 21979939
    4. Reg3alpha stimulates beta-cell replication, by activating Akt kinase and increasing the levels of cyclin D1/CDK4. PMID: 19343564
  • 相关疾病：
    Overexpressed during the acute phase of pancreatitis.
  • 亚细胞定位：
    Secreted.
  • 组织特异性：
    Small intestine and pancreas.
  • 数据库链接：

    KEGG: mmu:19694

    STRING: 10090.ENSMUSP00000098829

    UniGene: Mm.2552

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九游平台-九游(中国) | 米兰体育-米兰体育（中国） | 开云手机在线登陆入口 | 江南官方体育网页版 | 开云网页版 | 开云官方在线入口 | 开云电子体育 | 开云官方链接 | 江南网页版页面登录 |