Mouse R-spondin-4(RSPO4) ELISA kit

产品详情

产品描述：

小鼠R脊椎蛋白4(RSPO4)酶联免疫试剂盒（CSB-EL020553MO）为双抗夹心法ELISA试剂盒，定量检测血清、血浆、组织匀浆样本中的RSPO4含量。RSPO4是重要靶点。它在胚胎发育、组织稳态维持等过程起关键作用。研究发现其通过与特定受体结合激活Wnt信号通路，影响细胞增殖与分化。在疾病方面，RSPO4异常表达和信号失调与肿瘤、皮肤病等发病机制相关。试剂盒检测范围为34.4 pg/mL-2200 pg/mL，适用于体外研究RSPO4在疾病模型中的动态变化，如基因编辑小鼠的蛋白表达验证、病理状态下信号通路的分子机制探索，或药物干预后生物标志物的定量评估，为发育生物学、肿瘤学及代谢研究领域提供可靠的实验工具。本品仅用于科研，不用于临床诊断，产品具体参数及操作步骤详见产品说明书。
别名：

Rspo4R-spondin-4 ELISA kit; Cysteine-rich and single thrombospondin domain-containing protein 4 ELISA kit; Cristin-4 ELISA kit; mCristin-4 ELISA kit; Roof plate-specific spondin-4 ELISA kit
缩写：

RSPO4
Uniprot No.：

Q8BJ73
种属：

Mus musculus (Mouse)
样本类型：

serum, plasma, tissue homogenates
检测范围：

34.4 pg/mL-2200 pg/mL
灵敏度：

8.6 pg/mL
反应时间：

1-5h
样本体积：

50-100ul
检测波长：

450 nm
研究领域：

Developmental Biology
测定原理：

quantitative
测定方法：

Sandwich

精密度：

Intra-assay Precision (Precision within an assay): CV%<8%
Three samples of known concentration were tested twenty times on one plate to assess.
Inter-assay Precision (Precision between assays): CV%<10%
Three samples of known concentration were tested in twenty assays to assess.

线性度：

To assess the linearity of the assay, samples were spiked with high concentrations of mouse RSPO4 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
	Sample	Serum(n=4)
1:1	Average %	91
	Range %	87-95
1:2	Average %	97
	Range %	93-102
1:4	Average %	92
	Range %	85-96
1:8	Average %	97
	Range %	95-101

回收率：

The recovery of mouse RSPO4 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.

Sample Type	Average % Recovery	Range
Serum (n=5)	96	92-101
EDTA plasma (n=4)	87	83-93

标准曲线：

These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

功能：

Activator of the canonical Wnt signaling pathway by acting as a ligand for LGR4-6 receptors. Upon binding to LGR4-6 (LGR4, LGR5 or LGR6), LGR4-6 associate with phosphorylated LRP6 and frizzled receptors that are activated by extracellular Wnt receptors, triggering the canonical Wnt signaling pathway to increase expression of target genes. Also regulates the canonical Wnt/beta-catenin-dependent pathway and non-canonical Wnt signaling by acting as an inhibitor of ZNRF3, an important regulator of the Wnt signaling pathway.
亚细胞定位：

Secreted.
蛋白家族：

R-spondin family
数据库链接：

KEGG: mmu:228770

STRING: 10090.ENSMUSP00000041578

UniGene: Mm.159868