Mouse Pulmonary surfactant-associated protein A,SP-A ELISA Kit

Mouse Pulmonary surfactant-associated protein A,SP-A ELISA Kit

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中文名称：

小鼠肺表面活性物质相关蛋白A(SP-A)酶联免疫试剂盒
货号：

CSB-E08685m
规格：

96T/48T
价格：

￥3200/￥2500
其他：

CUSABIO Wins 2024 Bioz Rapid Star™ Award in the ELISA category

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产品描述：

CUSABIO小鼠肺表面活性物质相关蛋白A（SP-A）酶联免疫检测试剂盒（货号CSB-E08685m），专为科研领域设计，适用于血清、血浆及组织匀浆样本中SP-A蛋白的精准定量分析。SP-A是肺表面活性物质的关键功能蛋白，在调节肺泡表面张力、维持肺组织稳态及先天免疫防御中发挥重要作用，其表达水平与肺部炎症、急性肺损伤等呼吸系统病理机制密切相关。本试剂盒基于竞争法检测原理，通过高特异性抗体实现3.12 pg/mL至200 pg/mL的宽线性检测范围，具备优异的选择性和灵敏度。实验操作支持灵活选择血清、血浆或组织样本类型，尤其适用于动物模型研究中的肺功能评估、呼吸道疾病机制探索以及药物干预效果评价等科研场景，为研究者提供稳定可靠的检测工具。试剂盒包含预包被板、标准品及检测所需全套组分，严格遵循质量控制标准，确保实验数据的高度重复性。
别名：

Sftpa1 ELISA Kit; Sftp-1 ELISA Kit; Sftp1 ELISA Kit; Sftpa ELISA Kit; Pulmonary surfactant-associated protein A ELISA Kit; PSAP ELISA Kit; PSP-A ELISA Kit; SP-A ELISA Kit
缩写：

SFTPA1
Uniprot No.：

P35242
种属：

Mus musculus (Mouse)
样本类型：

serum, plasma, tissue homogenates
检测范围：

3.12 pg/mL-200 pg/mL
灵敏度：

0.78 pg/mL
反应时间：

1-5h
样本体积：

50-100ul
检测波长：

450 nm
研究领域：

Signal Transduction
测定原理：

quantitative
测定方法：

Competitive

精密度：

线性度：

To assess the linearity of the assay, samples were spiked with high concentrations of mouse SP-A in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
	Sample	Serum(n=4)
1:1000	Average %	84
	Range %	80-92
1:2000	Average %	96
	Range %	91-105
1:4000	Average %	98
	Range %	93-103
1:8000	Average %	93
	Range %	86-98

回收率：

The recovery of mouse SP-A spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.

Sample Type	Average % Recovery	Range
Serum (n=5)	90	87-95
EDTA plasma (n=4)	93	90-95

标准曲线：

These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

pg/ml	OD1	OD2	Average	Corrected
200	2.637	2.714	2.676	2.549
100	2.331	2.368	2.350	2.223
50	1.847	1.901	1.874	1.747
25	1.273	1.267	1.270	1.143
12.5	0.845	0.869	0.857	0.730
6.25	0.542	0.583	0.563	0.436
3.12	0.322	0.317	0.320	0.193
0	0.128	0.126	0.127

数据处理：

ELISA操作视频及标曲绘制软件下载
货期：

3-5 working days

Identification and Modulation of Microenvironment is Crucial for Effective MSC Therapy in Acute Lung Injury Islam D, et al,American journal of respiratory and critical care medicine,2018

功能：

In presence of calcium ions, it binds to surfactant phospholipids and contributes to lower the surface tension at the air-liquid interface in the alveoli of the mammalian lung and is essential for normal respiration. Enhances the expression of MYO18A/SP-R210 on alveolar macrophages.
亚细胞定位：

Secreted, extracellular space, extracellular matrix. Secreted, extracellular space, surface film.
蛋白家族：

SFTPA family
数据库链接：

STRING: 10090.ENSMUSP00000022314

UniGene: Mm.46062

Intra-assay Precision (Precision within an assay): CV%<8%

Three samples of known concentration were tested twenty times on one plate to assess.

Inter-assay Precision (Precision between assays): CV%<10%

Three samples of known concentration were tested in twenty assays to assess.