Mouse Pulmonary Surfactant-associated protein C (SP-C) ELISA kit

Mouse Pulmonary Surfactant-associated protein C (SP-C) ELISA kit

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中文名称：

小鼠肺表面活性物质相关蛋白C(SP-C)酶联免疫试剂盒
货号：

CSB-E12639m
规格：

96T/48T
价格：

￥3200/￥2500
其他：

CUSABIO Wins 2024 Bioz Rapid Star™ Award in the ELISA category

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产品描述：

小鼠肺表面活性物质相关蛋白C(SP-C)酶联免疫试剂盒（CSB-E12639m）为双抗夹心法ELISA试剂盒，定量检测血清、血浆、组织培养上清液、组织匀浆样本中的SFTPC含量。SFTPC编码表面活性蛋白C，是肺表面活性物质的重要成分，对维持肺泡稳定性至关重要。其突变会影响蛋白结构与功能，干扰肺表面活性物质代谢。相关研究聚焦突变致病机制，探索其在间质性肺疾病发病中的作用及潜在治疗靶点。试剂盒检测范围为2.3 ng/mL-150 ng/mL，适用于基础科研中探究肺部疾病模型（如肺纤维化、急性肺损伤）、肺泡上皮细胞功能研究或药物干预下SP - C的表达动态变化。其宽泛的样本兼容性支持体外细胞实验（培养上清）、动物模型（血清/血浆）及组织样本（肺匀浆）的多维度分析，为研究肺部生理病理机制提供可靠工具。本品仅用于科研，不用于临床诊断，产品具体参数及操作步骤详见产品说明书。
别名：

Sftpc ELISA Kit; Sftp2 ELISA Kit; Pulmonary surfactant-associated protein C ELISA Kit; SP-C ELISA Kit; Pulmonary surfactant-associated proteolipid SPL(Val) ELISA Kit; SP5 ELISA Kit
缩写：

SFTPC
Uniprot No.：

P21841
种属：

Mus musculus (Mouse)
样本类型：

serum, plasma, cell culture supernates, tissue homogenates
检测范围：

2.3 ng/mL-150 ng/mL
灵敏度：

0.58 ng/mL
反应时间：

1-5h
样本体积：

50-100ul
检测波长：

450 nm
研究领域：

Signal Transduction
测定原理：

quantitative
测定方法：

Sandwich

精密度：

线性度：

To assess the linearity of the assay, samples were spiked with high concentrations of mouse SP-C in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
	Sample	Serum(n=4)
1:1	Average %	86
	Range %	80-92
1:2	Average %	91
	Range %	85-96
1:4	Average %	95
	Range %	90-100
1:8	Average %	98
	Range %	92-102

回收率：

The recovery of mouse SP-C spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.

Sample Type	Average % Recovery	Range
Serum (n=5)	94	88-98
EDTA plasma (n=4)	96	90-100

标准曲线：

These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

ng/ml	OD1	OD2	Average	Corrected
150	2.147	2.052	2.100	2.009
75	1.693	1.576	1.635	1.544
37.5	1.121	1.044	1.083	0.992
18.8	0.648	0.639	0.644	0.553
9.4	0.429	0.424	0.427	0.336
4.7	0.295	0.279	0.287	0.196
2.3	0.194	0.179	0.187	0.096
0	0.092	0.090	0.091

数据处理：

ELISA操作视频及标曲绘制软件下载
货期：

3-5 working days

Pulmonary Surfactant Homeostasis Dysfunction Mediates Multiwalled Carbon Nanotubes Induced Lung Fibrosis via Elevating Surface Tension X Li,ACS nano,2023
GCN5L1 regulates pulmonary surfactant production by modulating lamellar body biogenesis and trafficking in mouse alveolar epithelial cells W Xu,Cellular & molecular biology letters,2023
Polyhexamethylene guanidine aerosol triggers pulmonary fibrosis concomitant with elevated surface tension via inhibiting pulmonary surfactant Xin Li,Journal of hazardous materials,2021
Differential effects of Nintedanib and Pirfenidone on lung alveolar epithelial cell function in ex vivo murine and human lung tissue cultures of pulmonary fibrosis Mareike Lehmann.et al,Respiratory Research,2018

功能：

Pulmonary surfactant associated proteins promote alveolar stability by lowering the surface tension at the air-liquid interface in the peripheral air spaces.
亚细胞定位：

Secreted, extracellular space, surface film.
数据库链接：

STRING: 10090.ENSMUSP00000022692

UniGene: Mm.24040

Intra-assay Precision (Precision within an assay): CV%<8%

Three samples of known concentration were tested twenty times on one plate to assess.

Inter-assay Precision (Precision between assays): CV%<10%

Three samples of known concentration were tested in twenty assays to assess.