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  4. Mouse Pulmonary Surfactant-associated protein B (SP-B) ELISA kit

Mouse Pulmonary Surfactant-associated protein B (SP-B) ELISA kit

文献引用 (2) 说明书下载
  • 中文名称：
    小鼠肺表面活性物质相关蛋白B(SP-B)酶联免疫试剂盒
  • 货号：
    CSB-E12638m
  • 规格：
    96T/48T
  • 价格：
    ￥3200/￥2500
  • 其他：
CUSABIO Wins 2024 Bioz Rapid Star™ Award in the ELISA category

产品详情

  • 产品描述：
    小鼠肺表面活性物质相关蛋白B(SP-B)酶联免疫试剂盒（CSB-E12638m）为双抗夹心法ELISA试剂盒，定量检测血清、血浆、组织匀浆样本中的SFTPB含量。小鼠肺表面活性物质相关蛋白B（SP-B）是肺泡表面活性物质的重要组成部分，对降低肺泡表面张力、维持呼吸功能至关重要。研究显示，SP-B的表达与肺损伤、肺部疾病的发生发展密切相关，其基因多态性可能影响肺部疾病的易感性和临床预后。通过构建SP-B转基因小鼠模型，有助于深入探究SP-B在肺部疾病中的作用机制。试剂盒检测范围为0.45 ng/mL-30 ng/mL，本产品适用于基础科研中探究肺部生理病理机制，例如构建急性肺损伤、肺纤维化或新生儿呼吸窘迫综合征等动物模型时，可通过检测SP - B的动态变化评估肺功能状态。本品仅用于科研，不用于临床诊断，产品具体参数及操作步骤详见产品说明书。
  • 别名：
    Sftpb ELISA Kit; Sftp3 ELISA Kit; Pulmonary surfactant-associated protein B ELISA Kit; SP-B ELISA Kit; Pulmonary surfactant-associated proteolipid SPL(Phe) ELISA Kit
  • 缩写：
    SFTPB
  • Uniprot No.：
  • 种属：
    Mus musculus (Mouse)
  • 样本类型：
    serum, plasma, tissue homogenates
  • 检测范围：
    0.45 ng/mL-30 ng/mL
  • 灵敏度：
    0.11 ng/mL
  • 反应时间：
    1-5h
  • 样本体积：
    50-100ul
  • 检测波长：
    450 nm
  • 研究领域：
    Signal Transduction
  • 测定原理：
    quantitative
  • 测定方法：
    Sandwich
  • 精密度：
    Intra-assay Precision (Precision within an assay): CV%<8%
    Three samples of known concentration were tested twenty times on one plate to assess.
    Inter-assay Precision (Precision between assays): CV%<10%
    Three samples of known concentration were tested in twenty assays to assess.
  • 线性度：
    To assess the linearity of the assay, samples were spiked with high concentrations of mouse SP-B in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
    SampleSerum(n=4)
    1:1Average %92
    Range %86-97
    1:2Average %97
    Range %92-102
    1:4Average %88
    Range %85-93
    1:8Average %89
    Range %83-94
  • 回收率：
    The recovery of mouse SP-B spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.
    Sample TypeAverage % RecoveryRange
    Serum (n=5) 9187-96
    EDTA plasma (n=4)10296-109
  • 标准曲线：
    These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.
    ng/mlOD1OD2AverageCorrected
    302.176 2.206 2.191 2.031
    151.511 1.544 1.528 1.368
    7.50.897 0.970 0.934 0.774
    3.750.612 0.618 0.615 0.455
    1.80.362 0.392 0.377 0.217
    0.90.279 0.267 0.273 0.113
    0.450.225 0.220 0.223 0.063
    00.156 0.163 0.160
  • 数据处理：
    ELISA操作视频及标曲绘制软件下载
  • 货期：
    3-5 working days

引用文献

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Sftpb Proteins

靶点详情

  • 功能：
    Pulmonary surfactant-associated proteins promote alveolar stability by lowering the surface tension at the air-liquid interface in the peripheral air spaces. SP-B increases the collapse pressure of palmitic acid to nearly 70 millinewtons per meter.
  • 基因功能参考文献：
    1. Surfactant protein B suppresses lung cancer progression by inhibiting secretory phospholipase A2 activity and arachidonic acid production. PMID: 28743125
    2. These data provided a plausible mechanism linking maternal vitamin D deficiency with altered postnatal lung function. PMID: 27121020
    3. Surfactant protein B had decreased levels in influenza A virus PR8-infected cells, which was confirmed by immunoblotting and immunofluorescence assays. PMID: 25965799
    4. macrophages participate in the repression of SFTPB expression by LPS, and that macrophage-released cytokines (including TNF) regulate the transcription factor CEBPB PMID: 23590297
    5. ErbB4 regulates the expression of surfactant protein B in fetal lung maturation, helping to prepare for the fetal-neonatal transition. PMID: 21317380
    6. The stimulatory effects of estrogen on Sftpb are under transcriptional control of ErbB4. PMID: 21777626
    7. SP-B contributes to innate host defense of the lung by supplementing the nonoxidant antimicrobial defenses of alveolar macrophages PMID: 20007532
    8. DNA methylation and chromatin modifications cooperate with Nkx2-1 to regulate Sftpb gene cell specific expression. PMID: 19906647
    9. formation of the intersubunit disulfide bridge is not critical for SP-B function PMID: 12133857
    10. The late asthmatic response is linked with increased surface tension and reduced surfactant protein B in mice. PMID: 12225952
    11. This protein inhibits endotoxin-induced lung inflammation. PMID: 12594064
    12. Reduction of alveolar SP-B content causes surfactant dysfunction and respiratory failure, indicating that SP-B is required for postnatal lung function. PMID: 12639841
    13. SP-B expression is regulated by PPARgamma in the lung PMID: 12829715
    14. napsin, cathepsin H, and at least one other enzyme are involved in maturation of the biologically active SP-B peptide PMID: 14766755
    15. Results indicate that hypoxia-induced mitogenic factor induces surfactant protein B and C production in mouse lungs and cell lines via activation of phosphatidylinositol 3-kinase/Akt and ERK1/2 mitogen-activated protein kinases. PMID: 16166744
    16. absence of HO-1 in the lung parenchyma, not in bone marrow-derived inflammatory cells, was responsible for enhanced SP-B downregulation and severe physiologic lung dysfunction PMID: 16309574
    17. Via promoter mutation analysis, adjacent TTF-1 binding sites within the proximal promoter region of SP-C were found to be essential for TTF-1/TAP26-enhanced SP-C promoter activity. PMID: 16630564
    18. SP-B controls surface tension by forming a surfactant phospholipid film that regulates shape and nonspecific phagocytic activity of alveolar macrophages on the alveolar surface. PMID: 16632521
    19. ABCA3 is necessary for lamellar body biogenesis, surfactant protein-B processing, and lung development late in gestation. PMID: 17540762
    20. Three novel Sp1 binding motifs were identified in the mouse SP-B promoter. PMID: 19096034
    21. Results support the idea that maintenance of surfactant-associated protein B expression is critical to survival during acute lung injury. PMID: 19131640
    22. The surface tension-lowering properties of Sftpb play an important role in the regulation of pulmonary vascular perfusion. PMID: 19202005
    23. Maintaining Sftpb is critical to survival during acute lung injury PMID: 19131640

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  • 亚细胞定位：
    Secreted, extracellular space, surface film.
  • 数据库链接：

    KEGG: mmu:20388

    STRING: 10090.ENSMUSP00000066805

    UniGene: Mm.46033

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