Mouse Protein notum homolog(NOTUM) ELISA kit

产品详情

Intra-assay Precision (Precision within an assay): CV%<8%

Three samples of known concentration were tested twenty times on one plate to assess.

Inter-assay Precision (Precision between assays): CV%<10%

Three samples of known concentration were tested in twenty assays to assess.

产品描述：

小鼠notum蛋白同源物(NOTUM)酶联免疫试剂盒（CSB-EL015955MO）为双抗夹心法ELISA试剂盒，定量检测血清、血浆、组织匀浆、组织培养上清液样本中的NOTUM含量。NOTUM是一个重要靶点。它是分泌性蛋白，可调节Wnt信号通路。异常激活的Wnt信号与多种疾病相关，如肿瘤、纤维化等。对NOTUM的研究，主要围绕其对Wnt信号的调控机制，期望通过干预该靶点为相关疾病治疗提供新思路。试剂盒检测范围为0.156 ng/mL-10 ng/mL，本试剂盒适用于体外研究NOTUM在胚胎发育、干细胞分化以及骨代谢相关疾病模型中的表达动态，尤其适合评估不同处理条件下（如基因编辑、药物干预等）细胞或组织内源性NOTUM的分泌水平变化，为探索Wnt信号通路调控机制提供可靠工具。本品仅用于科研，不用于临床诊断，产品具体参数及操作步骤详见产品说明书。
别名：

NotumPalmitoleoyl-protein carboxylesterase NOTUM ELISA Kit; EC 3.1.1.98 ELISA Kit
缩写：

NOTUM
Uniprot No.：

Q8R116
种属：

Mus musculus (Mouse)
样本类型：

serum, plasma, tissue homogenates, cell culture supernates
检测范围：

0.156 ng/mL-10 ng/mL
灵敏度：

0.039 ng/mL
反应时间：

1-5h
样本体积：

50-100ul
检测波长：

450 nm
研究领域：

Neuroscience
测定原理：

quantitative
测定方法：

Sandwich

精密度：

线性度：

To assess the linearity of the assay, samples were spiked with high concentrations of mouse NOTUM in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
	Sample	Serum(n=4)
1:1	Average %	109
	Range %	104-113
1:2	Average %	93
	Range %	89-99
1:4	Average %	100
	Range %	96-104
1:8	Average %	88
	Range %	84-92

回收率：

The recovery of mouse NOTUM spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.

Sample Type	Average % Recovery	Range
Serum (n=5)	85	81-89
EDTA plasma (n=4)	98	94-102

标准曲线：

These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

功能：

Carboxylesterase that acts as a key negative regulator of the Wnt signaling pathway by specifically mediating depalmitoleoylation of WNT proteins. Serine palmitoleoylation of WNT proteins is required for efficient binding to frizzled receptors.
基因功能参考文献：
1. Notum influences mesenchymal cell differentiation by generating a barrier for Wnt ligands produced and secreted by airway epithelial cells to attenuate Wnt signaling. PMID: 29428562
2. The findings support a significant role for NOTUM in modulating WNT signaling pathways that have pleiotropic effects on tooth and kidney development. PMID: 26926082
3. Data show that notum and glypican-1 and glypican-3 gene expression during colorectal cancer (CRC) development and present evidence to suggest them as potential new biomarkers of CRC pathogenesis. PMID: 26517809
4. Male mice deficient in Notum specifically in the liver develop metabolic dysfunctions implicating Notum in the development of Type 2 diabetes. PMID: 26974334
5. Describes the human ortholog, and analyzes both the human and mouse promoters. PMID: 18429952
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亚细胞定位：

Secreted.
蛋白家族：

Pectinacetylesterase family, Notum subfamily
组织特异性：

Widely expressed. Expressed in lung, ovary, kidney, liver and brain. Not detected in thymus, heart, spleen, stomach, skeletal muscle and bone marrow.
数据库链接：

KEGG: mmu:77583

STRING: 10090.ENSMUSP00000101783

UniGene: Mm.32839