Mouse Protein disulfide-isomerase A4(PDIA4) ELISA kit

Mouse Protein disulfide-isomerase A4(PDIA4) ELISA kit

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中文名称：

小鼠蛋白质二硫键异构酶A4(PDIA4)酶联免疫试剂盒
货号：

CSB-EL017722MO
规格：

96T/48T
价格：

￥3600/￥2500
其他：

CUSABIO Wins 2024 Bioz Rapid Star™ Award in the ELISA category

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产品描述：

小鼠蛋白质二硫键异构酶A4(PDIA4)酶联免疫试剂盒（CSB-EL017722MO）为双抗夹心法ELISA试剂盒，定量检测血清、血浆、组织匀浆、细胞裂解物样本中的PDIA4含量。PDIA4作为靶点有重要研究意义。其参与蛋白质折叠、内质网应激反应等过程。研究围绕其在细胞内的分子作用机制，探索它与疾病发生发展的关系，如在癌症等疾病中可能有异常表达，为药物研发、疾病治疗靶点的确定提供了方向。试剂盒检测范围为0.312 ng/mL-20 ng/mL，适用于基础研究中探索PDIA4在细胞应激反应、内质网功能调控中的分子机制，或用于评估疾病模型动物体内PDIA4表达水平与病理进程的关联性，为蛋白质质量控制网络研究及靶向药物开发提供可靠工具。本品仅用于科研，不用于临床诊断，产品具体参数及操作步骤详见产品说明书。
别名：

Pdia4 ELISA Kit; Cai ELISA Kit; Erp72Protein disulfide-isomerase A4 ELISA Kit; EC 5.3.4.1 ELISA Kit; Endoplasmic reticulum resident protein 72 ELISA Kit; ER protein 72 ELISA Kit; ERp-72 ELISA Kit; ERp72 ELISA Kit
缩写：

PDIA4
Uniprot No.：

P08003
种属：

Mus musculus (Mouse)
样本类型：

serum, plasma, tissue homogenates, cell lysates
检测范围：

0.312 ng/mL-20 ng/mL
灵敏度：

0.078 ng/mL
反应时间：

1-5h
样本体积：

50-100ul
检测波长：

450 nm
研究领域：

Others
测定原理：

quantitative
测定方法：

Sandwich

精密度：

线性度：

To assess the linearity of the assay, samples were spiked with high concentrations of mouse PDIA4 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
	Sample	Serum(n=4)
1:1	Average %	88
	Range %	81-92
1:2	Average %	100
	Range %	93-104
1:4	Average %	90
	Range %	85-95
1:8	Average %	99
	Range %	91-103

回收率：

The recovery of mouse PDIA4 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.

Sample Type	Average % Recovery	Range
Serum (n=5)	92	85-96
EDTA plasma (n=4)	95	89-100

标准曲线：

These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

ng/ml	OD1	OD2	Average	Corrected
20	2.654	2.543	2.599	2.417
10	2.135	2.077	2.106	1.924
5	1.345	1.332	1.339	1.157
2.5	0.824	0.843	0.834	0.652
1.25	0.526	0.545	0.536	0.354
0.625	0.359	0.369	0.364	0.182
0.312	0.278	0.266	0.272	0.090
0	0.184	0.180	0.182

数据处理：

ELISA操作视频及标曲绘制软件下载
货期：

3-5 working days

基因功能参考文献：
1. activated by IL11 in placenta PMID: 28487027
2. PDIA4 as a negative regulator of cancer cell apoptosis. PMID: 28534513
3. ERp72 functions separately from protein disulfide isomerase and ERp57 in supporting platelet aggregation. Defective aggregation of ERp72-null platelets was recovered by ERp72, but not other thiol isomerases. PMID: 28576878
4. A network of endoplasmic reticulum ERp57, ERp72, and ERp29 facilitate polyomavirus infection. PMID: 21159867
5. There were increased numbers of ERp72-immunoreactive cells in the dorsal and lateral cortex during sleep deprivation. PMID: 12535952
6. These results suggest that domains 1 and 2 of ERp72 contribute to catalytic efficacy, and domain 3 to binding affinity. PMID: 16333982
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亚细胞定位：

Endoplasmic reticulum lumen. Melanosome.
蛋白家族：

Protein disulfide isomerase family
数据库链接：

KEGG: mmu:12304

STRING: 10090.ENSMUSP00000076521

UniGene: Mm.2442

Intra-assay Precision (Precision within an assay): CV%<8%

Three samples of known concentration were tested twenty times on one plate to assess.

Inter-assay Precision (Precision between assays): CV%<10%

Three samples of known concentration were tested in twenty assays to assess.