Your Good Partner in Biology Research

中文简体

CSF2｜ CTLA4｜ DLL3｜ EGFR｜ IL4｜ IL4R｜ LAG3｜ MSTN｜ NECTIN4｜ NT5E｜ SEZ6｜ TM4SF1｜ TNFSF13｜ TPO

  1. 首页
  2. 试剂盒
  3. 酶联免疫试剂盒
  4. Mouse Protein disulfide-isomerase(P4HB) ELISA kit

Mouse Protein disulfide-isomerase(P4HB) ELISA kit

说明书下载
  • 中文名称：
    小鼠蛋白质二硫键异构酶(P4HB)酶联免疫试剂盒
  • 货号：
    CSB-EL017342MO
  • 规格：
    96T/48T
  • 价格：
    ￥3600/￥2500
  • 其他：
CUSABIO Wins 2024 Bioz Rapid Star™ Award in the ELISA category

产品详情

  • 产品描述：
    小鼠蛋白质二硫键异构酶(P4HB)酶联免疫试剂盒（CSB-EL017342MO）为双抗夹心法ELISA试剂盒，定量检测血清、血浆、组织匀浆样本中的P4HB含量。P4HB 即脯氨酰 4-羟化酶β亚基，是内质网内的多功能蛋白。其参与胶原蛋白合成，对维持细胞外基质稳定至关重要。在研究机制上，它作为伴侣蛋白促进蛋白质折叠，还参与氧化还原反应。P4HB 与多种疾病相关，是潜在的治疗靶点。试剂盒检测范围为0.312 ng/mL-20 ng/mL，为研究蛋白质折叠调控机制、氧化应激相关疾病模型（如肝脏疾病或代谢异常）以及内质网功能相关分子通路提供可靠工具；适用于体外实验中探索P4HB在细胞生理病理过程中的表达变化及其分子互作研究，满足基础科研中对目标蛋白定量分析的需求。本品仅用于科研，不用于临床诊断，产品具体参数及操作步骤详见产品说明书。
  • 别名：
    P4hb ELISA Kit; Pdia1Protein disulfide-isomerase ELISA Kit; PDI ELISA Kit; EC 5.3.4.1 ELISA Kit; Cellular thyroid hormone-binding protein ELISA Kit; Endoplasmic reticulum resident protein 59 ELISA Kit; ER protein 59 ELISA Kit; ERp59 ELISA Kit; Prolyl 4-hydroxylase subunit beta ELISA Kit; p55 ELISA Kit
  • 缩写：
    P4HB
  • Uniprot No.：
  • 种属：
    Mus musculus (Mouse)
  • 样本类型：
    serum, plasma, tissue homogenates
  • 检测范围：
    0.312 ng/mL-20 ng/mL
  • 灵敏度：
    0.078 ng/mL
  • 反应时间：
    1-5h
  • 样本体积：
    50-100ul
  • 检测波长：
    450 nm
  • 研究领域：
    Cancer
  • 测定原理：
    quantitative
  • 测定方法：
    Sandwich
  • 精密度：
    Intra-assay Precision (Precision within an assay): CV%<8%
    Three samples of known concentration were tested twenty times on one plate to assess.
    Inter-assay Precision (Precision between assays): CV%<10%
    Three samples of known concentration were tested in twenty assays to assess.
  • 线性度：
    To assess the linearity of the assay, samples were spiked with high concentrations of mouse P4HB in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
    　 Sample Serum(n=4)
    1:1 Average % 94
    Range % 87-98
    1:2 Average % 103
    Range % 96-108
    1:4 Average % 92
    Range % 85-97
    1:8 Average % 101
    Range % 95-107
  • 回收率：
    The recovery of mouse P4HB spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.
    Sample Type Average % Recovery Range
    Serum (n=5) 89 84-95
    EDTA plasma (n=4) 99 91-105
  • 标准曲线：
    These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.
    ng/ml OD1 OD2 Average Corrected
    20 2.248 2.175 2.212 2.044
    10 1.615 1.584 1.600 1.432
    5 1.016 0.967 0.992 0.824
    2.5 0.545 0.536 0.541 0.373
    1.25 0.342 0.358 0.350 0.182
    0.625 0.251 0.243 0.247 0.079
    0.312 0.215 0.210 0.213 0.045
    0 0.171 0.165 0.168 　
  • 数据处理：
    ELISA操作视频及标曲绘制软件下载
  • 货期：
    3-5 working days

产品评价

相关产品

P4hb antibodies

P4hb Proteins

靶点详情

  • 功能：
    This multifunctional protein catalyzes the formation, breakage and rearrangement of disulfide bonds. At the cell surface, seems to act as a reductase that cleaves disulfide bonds of proteins attached to the cell. May therefore cause structural modifications of exofacial proteins. Inside the cell, seems to form/rearrange disulfide bonds of nascent proteins. At high concentrations, functions as a chaperone that inhibits aggregation of misfolded proteins. At low concentrations, facilitates aggregation (anti-chaperone activity). May be involved with other chaperones in the structural modification of the TG precursor in hormone biogenesis. Also acts a structural subunit of various enzymes such as prolyl 4-hydroxylase and microsomal triacylglycerol transfer protein MTTP. Receptor for LGALS9; the interaction retains P4HB at the cell surface of Th2 T helper cells, increasing disulfide reductase activity at the plasma membrane, altering the plasma membrane redox state and enhancing cell migration.
  • 基因功能参考文献：
    1. Unfolded protein response caused by protein misfolding, may lead to PDI-dependent NOX activation and contribute to neurotoxicity in neurodegenerative diseases including ALS. PMID: 23118353
    2. PDI from endothelial cells is required for fibrin generation in vivo. PMID: 20668226
    3. Protein disulfide isomerase (PDI) on the platelet surface is recognized by anti-dengue virus NS1 antibodies. PMID: 19822367
  • 亚细胞定位：
    Endoplasmic reticulum. Endoplasmic reticulum lumen. Melanosome. Cell membrane; Peripheral membrane protein.
  • 蛋白家族：
    Protein disulfide isomerase family
  • 数据库链接：

    KEGG: mmu:18453

    STRING: 10090.ENSMUSP00000026122

    UniGene: Mm.16660

Most popular with customers

产品中心
特别关注


X
九游平台-九游(中国) | 米兰体育-米兰体育（中国） | 开云手机在线登陆入口 | 江南官方体育网页版 | 开云网页版 | 开云官方在线入口 | 开云电子体育 | 开云官方链接 | 江南网页版页面登录 |
九游平台-九游(中国) | 米兰体育-米兰体育（中国） | 开云手机在线登陆入口 | 江南官方体育网页版 | 开云网页版 | 开云官方在线入口 | 开云电子体育 | 开云官方链接 | 江南网页版页面登录 |