Mouse Platelet-derived growth factor DD(PDGF-DD) ELISA kit

产品详情

产品描述：

小鼠血小板衍生生长因子DD(PDGF-DD) 酶联免疫试剂盒（CSB-EL017711MO）为双抗夹心法ELISA试剂盒，定量检测血清、血浆、组织匀浆样本中的PDGF-DD含量。PDGF - DD是一种重要的靶点。它属血小板衍生生长因子家族，在细胞增殖、迁移与血管生成等过程起关键作用。研究机制方面，主要聚焦其与受体结合后的信号传导通路，以及在肿瘤、纤维化等疾病中的异常激活，为相关疾病治疗提供新方向。试剂盒检测范围为0.156 ng/mL-10 ng/mL，适用于小鼠模型研究中PDGF-DD的动态监测，如探究其在组织修复、纤维化进程或肿瘤微环境中的调控作用，支持心血管疾病、肿瘤生物学及再生医学等领域的科研实验。本品仅用于科研，不用于临床诊断，产品具体参数及操作步骤详见产品说明书。
缩写：

PDGF-DD
种属：

Mus musculus (Mouse)
样本类型：

serum, plasma, tissue homogenates
检测范围：

0.156 ng/mL-10 ng/mL
灵敏度：

0.039 ng/mL
反应时间：

1-5h
样本体积：

50-100ul
检测波长：

450 nm
研究领域：

Others
测定原理：

quantitative
测定方法：

Sandwich

精密度：

Intra-assay Precision (Precision within an assay): CV%<8%
Three samples of known concentration were tested twenty times on one plate to assess.
Inter-assay Precision (Precision between assays): CV%<10%
Three samples of known concentration were tested in twenty assays to assess.

线性度：

To assess the linearity of the assay, samples were spiked with high concentrations of mouse PDGF-DD in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
	Sample	Serum(n=4)
1:1	Average %	93
	Range %	89-96
1:2	Average %	102
	Range %	98-106
1:4	Average %	88
	Range %	84-93
1:8	Average %	100
	Range %	95-104

回收率：

The recovery of mouse PDGF-DD spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.

Sample Type	Average % Recovery	Range
Serum (n=5)	86	83-89
EDTA plasma (n=4)	97	93-100

标准曲线：

These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.