Mouse Plastin-2(LCP1) ELISA kit

Mouse Plastin-2(LCP1) ELISA kit

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中文名称：

小鼠网素-2(LCP1)酶联免疫试剂盒
货号：

CSB-EL012824MO
规格：

96T/48T
价格：

￥3600/￥2500
其他：

CUSABIO Wins 2024 Bioz Rapid Star™ Award in the ELISA category

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产品描述：

小鼠网素-2(LCP1)酶联免疫试剂盒（CSB-EL012824MO）为双抗夹心法ELISA试剂盒，定量检测血清、血浆、组织匀浆、细胞裂解物样本中的LCP1含量。LCP1即淋巴细胞特异性蛋白1，在免疫系统中扮演重要角色。其表达异常和多种疾病相关，如肿瘤、炎症等。研究发现它参与细胞的迁移、黏附等过程，通过与肌动蛋白相互作用影响细胞骨架的重组，为相关疾病诊治提供新思路。试剂盒检测范围为25 pg/mL-1600 pg/mL，特别适用于科研领域中对肿瘤转移模型、免疫细胞功能研究以及相关分子机制探索的实验需求，例如通过检测药物干预或基因编辑后LCP1的浓度变化，评估其对细胞迁移能力的调控作用。本品仅用于科研，不用于临床诊断，产品具体参数及操作步骤详见产品说明书。
别名：

Lcp1 ELISA Kit; Pls2 ELISA Kit; Plastin-2 ELISA Kit; 65 kDa macrophage protein ELISA Kit; L-plastin ELISA Kit; Lymphocyte cytosolic protein 1 ELISA Kit; LCP-1 ELISA Kit; pp65 ELISA Kit
缩写：

LCP1
Uniprot No.：

Q61233
种属：

Mus musculus (Mouse)
样本类型：

serum, plasma, tissue homogenates, cell lysates
检测范围：

25 pg/mL-1600 pg/mL
灵敏度：

6.25 pg/mL
反应时间：

1-5h
样本体积：

50-100ul
检测波长：

450 nm
研究领域：

Signal Transduction
测定原理：

quantitative
测定方法：

Sandwich

精密度：

线性度：

To assess the linearity of the assay, samples were spiked with high concentrations of mouse LCP1 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
	Sample	Serum(n=4)
1:1	Average %	94
	Range %	90-98
1:2	Average %	99
	Range %	94-103
1:4	Average %	84
	Range %	80-88
1:8	Average %	90
	Range %	86-94

回收率：

The recovery of mouse LCP1 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.

Sample Type	Average % Recovery	Range
Serum (n=5)	89	84-93
EDTA plasma (n=4)	106	101-113

标准曲线：

These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

pg/ml	OD1	OD2	Average	Corrected
1600	2.612	2.537	2.575	2.434
800	2.142	2.056	2.099	1.958
400	1.471	1.567	1.519	1.378
200	0.874	0.905	0.890	0.749
100	0.534	0.579	0.557	0.416
50	0.290	0.279	0.285	0.144
25	0.229	0.249	0.239	0.098
0	0.144	0.137	0.141

数据处理：

ELISA操作视频及标曲绘制软件下载
货期：

3-5 working days

功能：

Actin-binding protein. Plays a role in the activation of T-cells in response to costimulation through TCR/CD3 and CD2 or CD28. Modulates the cell surface expression of IL2RA/CD25 and CD69.
基因功能参考文献：
1. the actin-bundling protein L-plastin (LPL) is required for the perinatal development of alveolar macrophages. PMID: 27758872
2. Results identify L-plastin as a key effector of Mst1 and establish a novel mechanism linking a signaling intermediate to an actin-binding protein critical to T cell migration. PMID: 27465533
3. L-plastin modulates both T- and B-cell function during the germinal center reaction and the production of T-cell-dependent antibody responses. PMID: 23589339
4. L-plastin may participate in signaling that enables lymphocyte transmigration PMID: 21832165
5. LPL-dependent actin bundling facilitates the formation of lamellipodia and normal immunological synapses and thereby enables T cell activation. PMID: 21076065
6. Regulation of sealing ring formation by L-plastin and cortactin in osteoclasts. PMID: 20650888
7. We thus identify L-plastin as a molecule critical for CCR7-mediated t-cell motility but dispensable for early CCR7 signaling. PMID: 20194718
8. plastin plays a role in host defense distinguishes integrin signaling from cell adhesion and spreading. PMID: 12871642
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亚细胞定位：

Cytoplasm, cytoskeleton. Cell projection. Cell junction. Cell projection, ruffle membrane; Peripheral membrane protein; Cytoplasmic side.
数据库链接：

KEGG: mmu:18826

STRING: 10090.ENSMUSP00000116271

UniGene: Mm.153911

Intra-assay Precision (Precision within an assay): CV%<8%

Three samples of known concentration were tested twenty times on one plate to assess.

Inter-assay Precision (Precision between assays): CV%<10%

Three samples of known concentration were tested in twenty assays to assess.