Mouse Plasma protease C1 inhibitor(SERPING1) ELISA kit

Mouse Plasma protease C1 inhibitor(SERPING1) ELISA kit

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中文名称：

小鼠血浆蛋白酶C1抑制剂(SERPING1)酶联免疫试剂盒
货号：

CSB-EL021086MO
规格：

96T/48T
价格：

￥3600/￥2500
其他：

CUSABIO Wins 2024 Bioz Rapid Star™ Award in the ELISA category

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产品描述：

本试剂盒（货号CSB-EL021086MO）采用夹心法酶联免疫吸附定量检测技术，专用于检测小鼠血清、血浆或组织匀浆样本中的血浆蛋白酶C1抑制剂（SERPING1）含量。SERPING1作为补体系统和凝血级联反应的关键调节因子，在维持免疫稳态和调控炎症反应中发挥重要作用。试剂盒检测灵敏度达5.6 ng/mL，线性检测范围为5.6-360 ng/mL，可精准量化生理或病理条件下生物样本中SERPING1的表达水平。实验采用预包被特异性抗体的96孔板，通过双抗体夹心法捕获目标蛋白，配合标准化的显色反应实现定量分析，具有操作简便、重复性好的特点。适用于基础医学研究中探索免疫调节机制、炎症性疾病模型建立、凝血功能相关实验以及药物干预效果评估等科研场景，为深入解析SERPING1在补体激活、血管通透性调节等生物学过程中的作用提供可靠工具。
别名：

Serping1 ELISA Kit; C1nh ELISA Kit; Plasma protease C1 inhibitor ELISA Kit; C1 Inh ELISA Kit; C1Inh ELISA Kit; C1 esterase inhibitor ELISA Kit; C1-inhibiting factor ELISA Kit; Serpin G1 ELISA Kit
缩写：

SERPING1
Uniprot No.：

P97290
种属：

Mus musculus (Mouse)
样本类型：

serum, plasma, tissue homogenates
检测范围：

5.6 ng/mL-360 ng/mL
灵敏度：

1.4 ng/mL
反应时间：

1-5h
样本体积：

50-100ul
检测波长：

450 nm
研究领域：

Blood Coagulation
测定原理：

quantitative
测定方法：

Sandwich

精密度：

线性度：

To assess the linearity of the assay, samples were spiked with high concentrations of mouse SERPING1 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
	Sample	Serum(n=4)
1:1000	Average %	100
	Range %	95-105
1:2000	Average %	104
	Range %	101-107
1:4000	Average %	83
	Range %	80-86
1:8000	Average %	98
	Range %	95-103

回收率：

The recovery of mouse SERPING1 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.

Sample Type	Average % Recovery	Range
Serum (n=5)	88	85-92
EDTA plasma (n=4)	96	93-99

标准曲线：

These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

ng/ml	OD1	OD2	Average	Corrected
360	2.559	2.569	2.564	2.461
180	2.253	2.298	2.276	2.173
90	1.822	1.843	1.833	1.730
45	1.079	1.098	1.089	0.986
22.5	0.602	0.634	0.618	0.515
11.25	0.331	0.351	0.341	0.238
5.6	0.205	0.220	0.213	0.110
0	0.102	0.104	0.103

数据处理：

ELISA操作视频及标曲绘制软件下载
货期：

3-5 working days

功能：

Activation of the C1 complex is under control of the C1-inhibitor. It forms a proteolytically inactive stoichiometric complex with the C1r or C1s proteases. May play a potentially crucial role in regulating important physiological pathways including complement activation, blood coagulation, fibrinolysis and the generation of kinins. Very efficient inhibitor of FXIIa. May inhibit chymotrypsin and kallikrein.
基因功能参考文献：
1. C1INH prevented myocardial ischemia reperfusion injury in mice. PMID: 22705194
2. Transgenic mice have been generated that express human C1 inhibitor mRNA and protein under the control of the human promoter and regulatory elements; C1nh production is demonstrated in mouse brain, spleen, liver, heart, kidney, and lung. PMID: 12421980
3. In vivo, C1 inhibitor (C1INH) reduces the number of viable bacteria in the blood and peritoneal fluid and accelerates killing of bacteria by blood neutrophils and peritoneal macrophages. PMID: 17785834
4. C1-inhibitor limits neointimal plaque formation and inflammation. This may involve blockade of complement activation, inhibition of leukocyte recruitment, and reduced triglyceride levels PMID: 18071075
5. In addition to the protective activities mediated via inhibition of the complement system, these studies indicate that C1INH also plays a direct role in suppression of leukocyte transmigration into reperfused tissue. PMID: 18787060
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亚细胞定位：

Secreted.
蛋白家族：

Serpin family
数据库链接：

KEGG: mmu:12258

STRING: 10090.ENSMUSP00000023994

UniGene: Mm.38888

Intra-assay Precision (Precision within an assay): CV%<8%

Three samples of known concentration were tested twenty times on one plate to assess.

Inter-assay Precision (Precision between assays): CV%<10%

Three samples of known concentration were tested in twenty assays to assess.