Mouse Phosphorylated Adenosine Monophosphate Activated Protein Kinase, pAMPK ELISA Kit

产品详情

Intra-assay Precision (Precision within an assay): CV%<8%

Three samples of known concentration were tested twenty times on one plate to assess.

Inter-assay Precision (Precision between assays): CV%<10%

Three samples of known concentration were tested in twenty assays to assess.

产品描述：

小鼠磷酸化腺苷酸活化蛋白激酶(pAMPK)酶联免疫试剂盒（CSB-E14394m）为双抗夹心法ELISA试剂盒，定量检测血清、血浆、组织匀浆、组织培养上清液样本中的pAMPK 含量。pAMPK即磷酸化的腺苷酸活化蛋白激酶，是细胞能量代谢的关键调节因子。其活性变化能感应细胞内能量状态。研究机制主要围绕它通过磷酸化下游底物，调节糖、脂代谢等过程，在代谢性疾病等研究中是重要靶点。试剂盒检测范围为0.156 ng/mL-10 ng/mL，适用于能量代谢机制研究、糖尿病/肥胖相关模型分析以及药物开发中 AMPK 活化程度的评估本品仅用于科研，不用于临床诊断，产品具体参数及操作步骤详见产品说明书。
缩写：

pAMPK
种属：

Mus musculus (Mouse)
样本类型：

serum, plasma, tissue homogenates, cell culture supernates
检测范围：

0.156 ng/mL-10 ng/mL
灵敏度：

0.039 ng/mL
反应时间：

1-5h
样本体积：

50-100ul
检测波长：

450 nm
研究领域：

Others
测定原理：

quantitative
测定方法：

Sandwich

精密度：

线性度：

To assess the linearity of the assay, samples were spiked with high concentrations of mouse pAMPK in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
	Sample	Serum(n=4)
1:1	Average %	96
	Range %	92-101
1:2	Average %	102
	Range %	99-104
1:4	Average %	89
	Range %	87-92
1:8	Average %	94
	Range %	90-99

回收率：

The recovery of mouse pAMPK spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.

Sample Type	Average % Recovery	Range
Serum (n=5)	103	99-106
EDTA plasma (n=4)	95	90-101

标准曲线：

These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.